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The Surfactant-Induced Conformational and Activity Alterations in Rhizopus niveus Lipase

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Abstract

In this study, we have reported the effect of nonionic, anionic, cationic, and zwitterionic detergents on the enzymatic activity and structural stability of Rhizopus niveus lipase. Secondary structural changes were monitored by Far-UV CD which shows that surfactant induces helicity in the Rhizopus niveus lipase protein which was maximum in case of CTAB followed by SDS, CHAPS, and Brij-35. Similarly, tertiary structural changes were monitored by tryptophan fluorescence. We also carried out enzyme kinetics assays which showed that activity was enhanced by 1.5- and 1.1-fold in the presence of CHAPS and Brij-35, respectively. Furthermore, there was a decline in activity by 20 and 30 % in case of SDS and CTAB, respectively. These studies may be helpful in understanding detergent–lipase interaction in greater detail as lipases are used in many industrial processes.

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Acknowledgments

Authors are thankful for the financial support from Council for Scientific and Industrial Research (PA is recipient of CSIR-JRF and GR is recipient of CSIR-SRF), New Delhi and the Central facility of Interdisciplinary Biotechnology Unit, AMU Aligarh.

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Authors and Affiliations

  1. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India

    Parvez Alam, Gulam Rabbani & Rizwan Hasan Khan

  2. Laboratory of Immunology and Molecular Biology, Zoology Department, Faculty of Science, Assiut University, Assiut, Egypt

    Gamal Badr

  3. Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Cairo, Egypt

    Badr Mohamed Badr

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  1. Parvez Alam
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  2. Gulam Rabbani
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  3. Gamal Badr
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Correspondence to Rizwan Hasan Khan.

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Alam, P., Rabbani, G., Badr, G. et al. The Surfactant-Induced Conformational and Activity Alterations in Rhizopus niveus Lipase. Cell Biochem Biophys 71, 1199–1206 (2015). https://doi.org/10.1007/s12013-014-0329-2

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