Abstract
Mild to moderate dopaminergic (DA) neuronal death in substantia nigra pars compacta (SNc) as the main pathological hallmark of Parkinson’s disease (PD) is usually silent and does not produce marked clinical symptoms. In this study, we investigated the association between SNc DA neuronal loss and serum levels of total bilirubin (TB), selenium (Se), and zinc (Zn) in 6-hydroxydopamine (6-OHDA) animal model of PD. The neurotoxin of 6-OHDA was injected into the medial forebrain bundle of right hemisphere by stereotaxic surgery. Two conventional behavioral tests were carried out in several steps after the toxin to confirm the model reproduction and quantify severity and progress of 6-OHDA-induced PD. Blood samples were collected within 1 week before the toxin and in the second, fifth, and eighth weeks thereafter. Immunohistochemistry (IHC) assessments were performed on the rat’s brain to determine the severity of DA neuronal loss in SNc. The severity of behavioral symptoms and TB levels were progressively increased in 6-OHDA-treated rats. On the other hand, Se and Zn levels in them were lower than control. These changes were observed in rats with severe or mild behavioral symptoms. Also, IHC revealed that changes in TB, Se, and Zn associate with SNc DA neuronal loss but do not correlate with its severity. Significant changes in serum levels of TB, Se, and Zn in the mild SNc DA neuronal loss suggest them as valuable parameters for establishment of a serum profile for early detection of PD.
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Data Availability
The datasets used during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
Authors thank Miss Minaei for her assistance in immunohistochemistry studies and also Mrs. Dargahi for her assistance in blood collections.
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This study was supported by a grant-in-aid for scientific research from the Research Council of Qazvin University of Medical Sciences (grant number: IR.QUMS.REC.1397.083).
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Study was designed by Haghdoost-Yazdi H and Sophiabadi M. Blood sampling and measurements of the bilirubin, Se, and Zn were managed by Rastgoo N. All authors involved in modeling of the animals and immunohistochemical assessments. Data was analyzed by Rastgoo N and manuscript wrote by all authors.
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All procedures of the present study were carried out according to the guidelines of animal experiments of the Research Council at Qazvin University of Medical Sciences. All authors confirm all data presented in this manuscript and consent for its submission in Biological Trace Element Research.
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Sophiabadi, M., Rastgoo, N. & Haghdoost-Yazdi, H. Dopaminergic Neuronal Death in Substantia Nigra Associates with Serum Levels of Total Bilirubin, Selenium, and Zinc: Evidences from 6-Hydroxydopamine Animal Model of Parkinson’s Disease. Biol Trace Elem Res 200, 4058–4067 (2022). https://doi.org/10.1007/s12011-021-03012-6
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DOI: https://doi.org/10.1007/s12011-021-03012-6