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Synthesis of Cobalt Hydroxide Nano-flakes Functionalized with Glutamic Acid and Conjugated with Thiosemicarbazide for Anticancer Activities Against Human Breast Cancer Cells

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Abstract

In recent years, researchers were attracted to nanomaterials components for their potential role in cancer treatment. This study aimed to develop a novel and biocompatible cobalt hydroxide (Co(OH)2) nano-flakes that is functionalized by glutamic acid (Glu) and conjugated to thiosemicarbazide (TSC) for anticancer activities against human breast cancer MCF-7 cells. Physico-chemical properties of the Co(OH)2@Glu-TSC nanomaterial are characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X–ray (EDX) analysis, and Fourier-transform infrared (FT-IR) spectroscopy. MTT assay, flow cytometry, and caspase-3 activity analysis used for evaluating anticancer properties of the Co(OH)2@Glu-TSC nanomaterial. The MTT assay result showed cellular uptake of Co(OH)2@Glu-TSC and cell viability loss in a concentration-dependent. Results of flow cytometry and caspase-3 activity analysis indicated the stimulation of apoptosis through an increase in Caspase-3 and nucleus fragmentation. In general, our findings indicate the anticancer activities of Co(OH)2@Glu-TSC nanomaterial and so it can be considered as a new treatment for breast cancer. However, further in vivo studies are required to evaluate the accumulation of Co(OH)2@Glu-TSC nanomaterial in healthy organs, such as the liver, kidneys, brain, and testes, and potential toxic effects.

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Authors and Affiliations

  1. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

    Mona Bejarbaneh & Ali Salehzadeh

  2. Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 41335–1914, Rasht, Iran

    Zeinab Moradi-Shoeili

  3. Department of Biology, Faculty of Sciences, Arak University, Arak, Iran

    Amir Jalali

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  1. Mona Bejarbaneh
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  2. Zeinab Moradi-Shoeili
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  3. Amir Jalali
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Correspondence to Ali Salehzadeh.

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Bejarbaneh, M., Moradi-Shoeili, Z., Jalali, A. et al. Synthesis of Cobalt Hydroxide Nano-flakes Functionalized with Glutamic Acid and Conjugated with Thiosemicarbazide for Anticancer Activities Against Human Breast Cancer Cells. Biol Trace Elem Res 198, 98–108 (2020). https://doi.org/10.1007/s12011-020-02049-3

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