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Serine Threonine-Protein Kinase-Derived IW13 Improves Lipid Metabolism via C/EBP-α/SREBP1/FAS Signaling Pathways in HFD-Induced Zebrafish In Vivo Larval Model

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Abstract

Obesity is linked to the development of major metabolic disorders such as type 2 diabetes, cardiovascular disease, and cancer. Recent research has focused on the molecular link between obesity and oxidative stress. Obesity impairs antioxidant function, resulting in dramatically increased reactive oxygen levels and apoptosis. In this study, we investigated the effect of IW13 peptide on inhibiting lipid accumulation and regulating the antioxidant mechanism to normalize the lipid metabolism in HFD induced zebrafish larvae. Our results showed that co-treatment with IW13 peptide showed a protective effect in HFD zebra fish larvae by increasing the survival and heart rate. However, IW13 peptide co-treatment reduced triglycerides and cholesterol levels while also restoring the SOD and CAT antioxidant enzymes. In addition, IW13 co-treatment inhibited the formation of lipid peroxidation and superoxide anion by regulating the glutathione level. Also, the results showed that IW13 specifically downregulated the expression of the lipogenic-specific genes (C/EBP-α, SREBP1, and FAS). The findings exhibited that the IW13 peptide with effective antioxidant and anti-obesity activity could act as a futuristic drug to treat obesity and oxidative stress-related diseases.

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Author notes

  1. Dr. Meivelu Moovendhan and Dr. Jesu Arockiaraj contributed equally in this work.

Authors and Affiliations

  1. Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, SIMATS, Chennai, Tamil Nadu, 600077, India

    Ajay Guru

  2. Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India

    Gokul Sudhakaran,  Boopathi Seenivasan &  Jesu Arockiaraj

  3. Department of Research & Innovation, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, 602105, India

    S. Karthick Raja Namasivayam

  4. Division of Molecular Immunology & Microbiology, CSIR-Central Drug Research Institute (CDRI), Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, Uttar Pradesh, 226031, India

    Mukesh Pasupulieti

  5. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Mukesh Pasupulieti

  6. Centre for Ocean Research, Col. Dr. Jeppiar Research Park, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600119, India

    Meivelu Moovendhan

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Contributions

AG: project administration, validation, writing—original draft; GS and SKRN: validation, writing—review and editing; BS and MP: conceptualization, data curation; MM and JA: conceptualization, data curation, supervision, writing—review and editing

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Correspondence to Jesu Arockiaraj or Meivelu Moovendhan.

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Ajay Guru, Gokul Sudhakaran, S. Karthick Raja Namasivayam et al. Serine Threonine-Protein Kinase-Derived IW13 Improves Lipid Metabolism via C/EBP-α/SREBP1/FAS Signaling Pathways in HFD-Induced Zebrafish In Vivo Larval Model. Appl Biochem Biotechnol 195, 4851–4863 (2023). https://doi.org/10.1007/s12010-023-04480-3

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