Abstract
In this study, we aimed to explore long non-coding RNA (lncRNA) sustained low-efficiency dialysis (SLED1) correlated with Bcl-2 apoptosis pathway in acute myeloid leukemia (AML). This study further aimed to determine its role in the regulation of AML progression and its action as a potential biomarker for better prognosis. AML microarray profiles GSE97485 and probe annotation from the Gene Expression Omnibus (GEO) database from the National Center for Biotechnology Information (NCBI) were detected using the GEO2R tool (http://www.ncbi.nlm.nih.gov/geo/geo2r/). The expression of AML was downloaded from the TCGA database (http://cancergenome.nih.gov/). The statistical analysis of the database was processed with R software. Bioinformatic analysis found that lncRNA SLED1 is highly expressed in AML patients and is associated with poor prognosis. We found that the increased SLED1 expression levels in AML were significantly correlated with FAB classification, human race, and age. Our study has shown that upregulation of SLED1 promoted AML cell proliferation and inhibited cell apoptosis in vitro; RNA sequencing showed increased expression of BCL-2 and indicated that SLED1 might promote the development of AML by regulating BCL-2. Our results showed that SLED1 could promote the proliferation and inhibit the apoptosis of AML cells. SLED1 might promote the development of AML by regulating BCL-2, but the mechanism involved in the progression of AML is unclear. SLED1 plays an important role in AML progression, may be applied as a rapid and economical AML prognostic indicator to predict the survival of AML patients, and help guide experiments for potential clinical drag targets.
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Data Availability
The datasets supporting the conclusions of this article are available in the Gene Expression Omnibus repository (GSE97485; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc = GSE97485) and the TCGA database (http://cancergenome.nih.gov/). The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- AML:
-
Acute myeloid leukemia
- lncRNAs:
-
Long non-coding RNAs
- SLED1:
-
Sustained low-efficiency dialysis
- RNA:
-
Ribonucleic acid
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The study was supported by grants from the award of the China Scholarship Council Visiting Scholar Grant to Jimo Jian (No. 201906225042) and The Youth Scientific Foundation of Qilu Hospital (Qingdao) of Shandong University (No. QDKY2015QN10).
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Conceptualization, Jimo Jian; formal analysis, Jimo JianNana Wang and Hongyuan Hao; methodology, Jimo Jian, Hongyuan Hao, and Qian Liu; project administration, Chenglu Yuan and Chunyan Ji; supervision, Fei Lu; writing—original draft, Jimo Jian; writing—review and editing, Fei Lu. All of the authors have read and approved the manuscript.
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Jian, J., Wang, N., Hao, H. et al. SLED1 Promoting Cell Proliferation and Inhibiting Apoptosis in Acute Myeloid Leukemia: a Study. Appl Biochem Biotechnol 195, 6633–6652 (2023). https://doi.org/10.1007/s12010-023-04421-0
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DOI: https://doi.org/10.1007/s12010-023-04421-0