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Antimicrobial and Antibiofilm Activity of Biosynthesized Silver Nanoparticles Against Beta-lactamase-Resistant Enterococcus faecalis

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Abstract

Due to the presence of antibiotic-resistant genes, treatment options of clinical isolates are exceedingly limited. This study was aimed to fabricate, optimize, characterize, and evaluate the action of silver nanoparticles (AgNPs) against a clinical isolate of Enterococcus faecalis. A combination of cell-free supernatant (C-FS) of the filamentous fungus Fusarium solani and Gram-negative Comamonas aquatica for AgNP formation was proposed; the antigrowth and antibiofilm of AgNPs against E. faecalis harboring blaTEM and blaCTX-M genes were assessed. The ratio of 1:2 v/v (C-FS:AgNO3) at pH 9.0 for 72 h in 1 mM AgNO3 were the optimal conditions for AgNP formation. UV–vis absorption peak appeared at 425 nm and the crystalline nature of synthesized particles was verified by X-ray diffraction (XRD). Fourier transform infrared spectroscopy (FTIR) analysis confirmed the interaction of protein molecules with the AgNPs. Transmission electron microscopy (TEM) analysis demonstrated that fabricated AgNPs were relatively monodispersed, approximately spherical, and of size 2–7.5 nm. blaTEM and blaCTX-M were detected in E. faecalis; the growth and biofilm of E. faecalis were significantly decreased by the action of 12.5 μg/mL AgNPs. This is the first study proposing alternative sources to form AgNPs via synergistic metabolites of F. solani and C. aquatica. The results here offer a foundation for developing an effective therapy using AgNPs against clinical pathogens.

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All data generated or analyzed during this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Iraq

    Bikhal Fattah, Huner Arif & Haider Hamzah

Authors
  1. Bikhal Fattah
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  2. Huner Arif
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  3. Haider Hamzah
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Contributions

This work is a part of Bikhal Fattah’s master thesis. BF carried out the experiments. Huner Arif is her second mentor and participated in drafting the manuscript. Haider Hamzah designed all the experiments and drafted the paper. All authors read and approved the final manuscript.

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Correspondence to Haider Hamzah.

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Fattah, B., Arif, H. & Hamzah, H. Antimicrobial and Antibiofilm Activity of Biosynthesized Silver Nanoparticles Against Beta-lactamase-Resistant Enterococcus faecalis. Appl Biochem Biotechnol 194, 2036–2046 (2022). https://doi.org/10.1007/s12010-022-03805-y

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