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Bioconversion of 5-Hydroxymethylfurfural (HMF) to 2,5-Furandicarboxylic Acid (FDCA) by a Native Obligate Aerobic Bacterium, Acinetobacter calcoaceticus NL14

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Abstract

2,5-Furandicarboxylic acid (FDCA), one of the top biomass-based platform chemical, is highly promising for resins and polymers, and it can be prepared from the bio-oxidation of hydroxymethyl furfural (HMF), which can be obtained mainly from lignocellulosic glucose that has a high production potential from not edible biomass.

A native strain, Acinetobacter calcoaceticus NL14, that could convert HMF into FDCA is used for combining degradation and fermentation by consolidated bioprocessing (CBP). In this study, it was observed that the initial HMF concentration and pH neutralizer played important roles in the bioconversion of HMF, 5 g/L of HMF could be converted by 100% within 48 h with 0.5 g/L sodium carbonate (Na2CO3) with the production of 0.31 g/L FDCA. Extra glucose and hydrogen peroxide (H2O2) addition could further promote the production of FDCA to 0.54 g/L with 100% HMF conversion and a higher conversion rate. This report could provide a potential native bacterium for furan chemicals bioconversion and bioelimination, especially for FDCA bioproduction.

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Funding

The research was supported by the financial support from the National Natural Science Foundation of China (31901270), the Scientific Research Start-up Funds of Nanjing Forestry University, China (163030127), the National Natural Science Funding of China (grant no. 31370573), and the Key Research and Development Program of Jiangsu (BE 2015758). This work is also financially supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing, 210037, People’s Republic of China

    Yequan Sheng, Xin Zhou & Yong Xu

  2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Yequan Sheng, Xin Zhou & Yong Xu

  3. Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing, 210037, People’s Republic of China

    Yequan Sheng, Xin Zhou & Yong Xu

  4. Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, OH, 45221, USA

    Xin Tan

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  1. Yequan Sheng
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Correspondence to Yong Xu.

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Sheng, Y., Tan, X., Zhou, X. et al. Bioconversion of 5-Hydroxymethylfurfural (HMF) to 2,5-Furandicarboxylic Acid (FDCA) by a Native Obligate Aerobic Bacterium, Acinetobacter calcoaceticus NL14. Appl Biochem Biotechnol 192, 455–465 (2020). https://doi.org/10.1007/s12010-020-03325-7

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