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Biological Impacts of Metal(II) Complex–Based DNA Probes Derived from Bidentate N,O Donor Schiff Base Ligand

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Abstract

In this article, we have reported the preparation and structural characterization of a new Schiff base ligand (E)-2-(((2,6-difluorophenyl)imino)methyl)phenol (HSBL) and its derived metal(II) complexes [Cu(SBL)2] (1), [Ni(SBL)2] (2) and [Pd(SBL)2] (3). Using various analytical and spectroscopic techniques, their structural properties have been appraised. The proposed chemical structure of HSBL has been confirmed by Single crystal XRD studies. Bidentate characteristic of HSBL and its coordination with metal(II) ions through the oxygen atom of the phenolic group and nitrogen atom of the azomethine group have been evaluated from the FT-IR spectral analysis. Pd(II) complex of HSBL (complex 3) has found to be efficient in bringing about the interaction with DNA as well as BSA molecules. The in vitro antimicrobial studies have been demonstrated that complex 3 has a superior antimicrobial activity than HSBL, complexes 1 and 2. According to the values of zone of inhibition, the antimicrobial ability has been increased in the order of 3 > 1 > 2 > HSBL. A significant decrease in percent cell viability has been suggested that complex 3 has remarkable cytotoxicity (IC50 = 15.7 ± 0.6 μg/mL) on human breast cancer (MCF-7) cells. Besides, their induced apoptosis pathway of cytotoxicity has been demonstrated by fluorescence staining techniques using AO/EB staining method. We hope this article will be very helpful for future research on the development of new anticancer agents.

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Acknowledgements

The authors honestly acknowledge to the management of Chikkanna Government Arts College and Mohamed Sathak Engineering College for their lab and instrumental facilities.

Funding

This work was supported by the DST-SERB (Grant No.: SB/FT/CS-130/2012).

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Authors and Affiliations

  1. PG and Research Department of Chemistry, Chikkanna Government Arts College, Tiruppur, Tamil Nadu, 641602, India

    Irudayaraj Bernadette Amali & Gurusamy Rajagopal

  2. Department of Chemistry, Hajee Karutha Rowther Howdia College, Uthamapalayam, Tamil Nadu, 625533, India

    Mookkandi Palsamy Kesavan

  3. Centre for Organic and Medicinal Chemistry, VIT University, Vellore, Tamil Nadu, 632014, India

    Vijayaparthasarathi Vijayakumar

  4. Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, 632014, India

    Ramalingam Rajasekaran

  5. Chemistry Research Centre, Mohamed Sathak Engineering College, Ramanathapuram (Dt), Kilakarai, Tamil Nadu, 623806, India

    Jegathalaprathaban Rajesh

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  1. Irudayaraj Bernadette Amali
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Amali, I.B., Kesavan, M.P., Vijayakumar, V. et al. Biological Impacts of Metal(II) Complex–Based DNA Probes Derived from Bidentate N,O Donor Schiff Base Ligand. Appl Biochem Biotechnol 190, 373–390 (2020). https://doi.org/10.1007/s12010-019-03110-1

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