Abstract
Anaerobic digestion of microalgal biomass for biogas production may be limited due to the cell wall resulting in an inefficient bioconversion. Enzymatic pretreatments are applied for inducing cell damage/lysis and organic matter solubilisation and this way increasing biogas production. We evaluated enzymatic pretreatments in different conditions for comparing in relation to cell wall rupture, increase of soluble material and increase in biogas production through anaerobic digestion performance in BMP assay. Chlorella sorokiniana cultures were subjected to three different enzymatic pretreatments, each under four different conditions of enzyme/substrate ratio, pH and application time. The results showed increases over 21% in biogas productions for all enzymatic pretreatments. Enzymatic pretreatment was effective at damaging microalgae cell wall, releasing organic compounds and increasing the rate and final methane yield in BMP tests. We observed a synergistic activity between the mixtures enzymes, which would depend on operational conditions used for each pretreatment.
Similar content being viewed by others
References
Bohutskyi, P., & Bouwer, E. (2013). Biogas production from algae and cyanobacteria through anaerobic digestion: a review, analysis, and research needs. In W. J. Lee (Ed.), Adv. Biofuels Bioprod (pp. 873–975). New York, NY: Springer New York. https://doi.org/10.1007/978-1-4614-3348-4_36.
Zamalloa, C., Vulsteke, E., Albrecht, J., & Verstraete, W. (2011). The techno-economic potential of renewable energy through the anaerobic digestion of microalgae. Bioresource Technology, 102(2), 1149–1158. https://doi.org/10.1016/j.biortech.2010.09.017.
Ward, A. J., Lewis, D. M., & Green, F. B. (2014). Anaerobic digestion of algae biomass: a review. Algal Research, 5, 204–214. https://doi.org/10.1016/j.algal.2014.02.001.
González-Fernández, C., Sialve, B., Bernet, N., & Steyer, J. P. (2012). Impact of microalgae characteristics on their conversion to biofuel. Part I: focus on cultivation and biofuel production. Biofuels, Bioprod. Biorefining., 6, 246–256. https://doi.org/10.1002/bbb.
González-Fernández, C., Sialve, B., Bernet, N., & Steyer, J. P. (2012). Impact of microalgae characteristics on their conversion to biofuel. Part II: focus on biomethane production. Biofuels, Bioprod. Biorefining, 6, 205–218. https://doi.org/10.1002/bbb.
Angelidaki, I., & Batstone, D. J. (2010). Anaerobic digestion. In Solid Waste Technology and Management, 1(2), 583–600.
Mendez, L., Mahdy, A., Timmers, R. A., Ballesteros, M., & González-Fernández, C. (2013). Enhancing methane production of Chlorella vulgaris via thermochemical pretreatments. Bioresource Technology, 149, 136–141. https://doi.org/10.1016/j.biortech.2013.08.136.
Passos, F., Uggetti, E., Carrère, H., & Ferrer, I. (2014). Pretreatment of microalgae to improve biogas production: a review. Bioresource Technology, 172, 403–412. https://doi.org/10.1016/j.biortech.2014.08.114.
Córdova, O., Santis, J., Ruiz-Fillipi, G., Zuñiga, M. E., Fermoso, F. G., & Chamy, R. (2018). Microalgae digestive pretreatment for increasing biogas production. Renewable and Sustainable Energy Reviews, 82, 2806–2813. https://doi.org/10.1016/j.rser.2017.10.005.
Yin, L. J., Jiang, S. T., Pon, S. H., & Lin, H. H. (2010). Hydrolysis of chlorella by Cellulomonas sp. YJ5 cellulases and its biofunctional properties. Journal of Food Science, 75(9), H317–H323. https://doi.org/10.1111/j.1750-3841.2010.01867.x.
Chen, C. Y., Der Bai, M., & Chang, J. S. (2013). Improving microalgal oil collecting efficiency by pretreating the microalgal cell wall with destructive bacteria. Biochemical Engineering Journal, 81, 170–176. https://doi.org/10.1016/j.bej.2013.10.014.
Muñoz, C., Hidalgo, C., Zapata, M., Jeison, D., Riquelme, C., & Rivas, M. (2014). Use of cellulolytic marine bacteria for enzymatic pretreatment in microalgal biogas production. Applied and Environmental Microbiology, 80(14), 4199–4206. https://doi.org/10.1128/AEM.00827-14.
Yun, Y. M., Kim, D. H., Oh, Y. K., Shin, H. S., & Jung, K. W. (2014). Application of a novel enzymatic pretreatment using crude hydrolytic extracellular enzyme solution to microalgal biomass for dark fermentative hydrogen production. Bioresource Technology, 159, 365–372. https://doi.org/10.1016/j.biortech.2014.02.129.
Zhang, Y. H. P., & Lynd, L. R. (2004). Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems. Biotechnology and Bioengineering, 88(7), 797–824. https://doi.org/10.1002/bit.20282.
Yang, B., Dai, Z., Ding, S.-Y., & Wyman, C. E. (2014). Enzymatic hydrolysis of cellulosic biomass. Biofuels., 2(4), 421–449. https://doi.org/10.4155/bfs.11.116.
APHA-AWWA-WPCF, Standard methods for the examination of water and wastewater, (20th Ed.)Washingt. (1999).
Percival Zhang, Y. H., Himmel, M. E., & Mielenz, J. R. (2006). Outlook for cellulase improvement: screening and selection strategies. Biotechnology Advances, 24(5), 452–481. https://doi.org/10.1016/j.biotechadv.2006.03.003.
Thomas, L., Joseph, A., & Gottumukkala, L. D. (2014). Xylanase and cellulase systems of Clostridium sp.: an insight on molecular approaches for strain improvement. Bioresource Technology, 158, 343–350. https://doi.org/10.1016/j.biortech.2014.01.140.
Passos, F., Hom-Diaz, A., Blanquez, P., Vicent, T., & Ferrer, I. (2016). Improving biogas production from microalgae by enzymatic pretreatment. Bioresource Technology, 199, 347–351. https://doi.org/10.1016/j.biortech.2015.08.084.
Mahdy, A., Ballesteros, M., & González-Fernández, C. (2016). Enzymatic pretreatment of Chlorella vulgaris for biogas production: influence of urban wastewater as a sole nutrient source on macromolecular profile and biocatalyst efficiency. Bioresource Technology, 199, 319–325. https://doi.org/10.1016/j.biortech.2015.08.080.
He, S., Fan, X., Katukuri, N. R., Yuan, X., Wang, F., & Guo, R. B. (2016). Enhanced methane production from microalgal biomass by anaerobic bio-pretreatment. Bioresource Technology, 204, 145–151. https://doi.org/10.1016/j.biortech.2015.12.073.
Córdova, O., Passos, F., & Chamy, R. (2018). Physical pretreatment methods for improving microalgae anaerobic biodegradability. Applied Biochemistry and Biotechnology, 185(1), 114–126. https://doi.org/10.1007/s12010-017-2646-6.
Sato, M., Murata, Y., Mizusawa, M., Iwahashi, H., & Oka, S. (2004). A simple and rapid dual-fluorescence viability assay for microalgae. Microbiology and Culture Collections, 20, 53–59 http://www.jscc-home.jp/journal/No20_2/No20_2_53.pdf.
Angelidaki, I., Alves, M., Bolzonella, D., Borzacconi, L., Campos, J. L., Guwy, A. J., Kalyuzhnyi, S., Jenicek, P., & van Lier, J. B. (2009). Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Science and Technology, 59(5), 927–934. https://doi.org/10.2166/wst.2009.040.
Donoso-Bravo, A., Pérez-Elvira, S. I., & Fdz-Polanco, F. (2010). Application of simplified models for anaerobic biodegradability tests. Evaluation of pre-treatment processes. Chemical Engineering Journal, 160(2), 607–614. https://doi.org/10.1016/j.cej.2010.03.082.
Doncaster, C. P., & Davey, A. J. H. (2007). Analysis of variance and covariance: how to choose and construct models for the life sciences. https://doi.org/10.1017/CBO9780511611377.
Kumar, R., Singh, S., & Singh, O. V. (2008). Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. Journal of Industrial Microbiology & Biotechnology, 35(5), 377–391. https://doi.org/10.1007/s10295-008-0327-8.
Moraïs, S., Barak, Y., Caspi, J., & Hadar, Y. (2010). Cellulase-xylanase synergy in designer cellulosomes for enhanced degradation of a complex cellulosic substrate. MBio., 1, 3–10. https://doi.org/10.1128/mBio.00285-10.Editor.
Gruber-Brunhumer, M. R., Jerney, J., Zohar, E., Nussbaumer, M., Hieger, C., Bochmann, G., Schagerl, M., Obbard, J. P., Fuchs, W., & Drosg, B. (2015). Acutodesmus obliquus as a benchmark strain for evaluating methane production from microalgae: influence of different storage and pretreatment methods on biogas yield. Algal Research, 12, 230–238. https://doi.org/10.1016/j.algal.2015.08.022.
Ometto, F., Quiroga, G., Psenicka, P., Whitton, R., Jefferson, B., & Villa, R. (2014). Impacts of microalgae pre-treatments for improved anaerobic digestion: thermal treatment, thermal hydrolysis, ultrasound and enzymatic hydrolysis. Water Research, 65, 350–361. https://doi.org/10.1016/j.watres.2014.07.040.
Merino, S. T., & Cherry, J. (2007). Progress and challenges in enzyme development for biomass utilization. Advances in Biochemical Engineering/Biotechnology, 108, 95–120. https://doi.org/10.1007/10_2007_066.
Funding
The authors want to thank Pontificia Universidad Católica de Valparaiso for the financial support. Olivia Córdova appreciates her scholarship funded by the CONICYT, Beca Nacional Doctorado, 21121012.
Author information
Authors and Affiliations
Contributions
OC conceived the study, designed, and performed the experiments, evaluated the data and drafted the manuscript. FP evaluated the data and drafted the manuscript. RC supervised the work and assisted in drafting the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Córdova, O., Passos, F. & Chamy, R. Enzymatic Pretreatment of Microalgae: Cell Wall Disruption, Biomass Solubilisation and Methane Yield Increase. Appl Biochem Biotechnol 189, 787–797 (2019). https://doi.org/10.1007/s12010-019-03044-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-019-03044-8