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Chemo-Enzymatic Epoxidation of Lallemantia IbericaSeed Oil: Process Development and Economic-Ecological Evaluation

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Abstract

The chemo-enzymatic epoxidation of Lallemantia iberica seed oil (LISO), a novel plant oil characterized by its exceptional high content of alpha-linolenic acid (> 60%), was developed using an immobilized lipase from Pseudozyma antarctica and hydrogen peroxide as oxidant. A statistical approach was used to study the effect of enzyme amount, temperature, time, and solvent amount on the oxirane oxygen content obtained during epoxidation. An oxirane oxygen content of 8.6 ± 0.2% corresponding to a yield of 82% was obtained under optimized conditions that were identified to be at an enzyme load of 8.2 g/mol of double bonds, a solvent amount of 56.4 wt.%, a temperature of 33 °C, and an incubation time of 17 h. In addition, the experimental investigation was combined with a techno-economic and ecological assessment gaining detailed information regarding cost structure and environmental impact for the chemo-enzymatic epoxidation of the novel plant oil.

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Acknowledgements

We like to thank Dr. J. Gottfriedsen (DRACOSA AG) for the supply of Lallemantia iberica seed oil and Novozymes A/S Denmark for the gift of Novozym® 435. We gratefully acknowledge the support of this work by BMEL represented by FNR within the joint research project »Integrated BioProduction« (FKZ of KIT IIP 22001909 and Fraunhofer IGB 22027407).

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Author notes

  1. Sophia Radloff

    Present address: University of Ulm, Helmholtzstr. 18, 89081, Ulm, Germany

  2. M. Fröhling

    Present address: TU Bergakademie Freiberg, Schloßplatz 1, 09599, Freiberg, Germany

  3. T. Hirth

    Present address: Karlsruhe Institute of Technology, Kaiserstr. 12, 76131, Karlsruhe, Germany

Authors and Affiliations

  1. Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstr. 12, 70569, Stuttgart, Germany

    Fabian Haitz, S. Rupp, T. Hirth & S. Zibek

  2. Karlsruhe Institute of Technology, Hertzstr. 16, 76187, Karlsruhe, Germany

    Sophia Radloff & M. Fröhling

  3. University of Stuttgart, Nobelstr. 12, 70569, Stuttgart, Germany

    S. Rupp

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  1. Fabian Haitz
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Correspondence to S. Zibek.

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Haitz, F., Radloff, S., Rupp, S. et al. Chemo-Enzymatic Epoxidation of Lallemantia IbericaSeed Oil: Process Development and Economic-Ecological Evaluation. Appl Biochem Biotechnol 185, 13–33 (2018). https://doi.org/10.1007/s12010-017-2630-1

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