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Production of Xylitol from d-Xylose by Overexpression of Xylose Reductase in Osmotolerant Yeast Candida glycerinogenes WL2002-5

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Abstract

Efficient bioconversion of d-xylose into various biochemicals is critical for the developing lignocelluloses application. In this study, we compared d-xylose utilization in Candida glycerinogenes WL2002-5 transformants expressing xylose reductase (XYL1) in d-xylose metabolism. C. glycerinogenes WL2002-5 expressing XYL1 from Schefferomyces stipitis can produce xylitol. Xylitol production by the recombinant strains was evaluated using a xylitol fermentation medium with glucose as a co-substrate. As glucose was found to be an insufficient co-substrate, various carbon sources were screened for efficient cofactor regeneration, and glycerol was found to be the best co-substrate. The effects of glycerol on the xylitol production rate by a xylose reductase gene (XYL1)-overexpressed mutant of C. glycerinogenes WL2002-5 were investigated. The XYL1-overexpressed mutant produced xylitol from d-xylose using glycerol as a co-substrate for cell growth and NAD (P) H regeneration: 100 g/L d-xylose was completely converted into xylitol when at least 20 g/L glycerol was used as a co-substrate. XYL1 overexpressed mutant grown on glycerol as co-substrate accumulated 2.1-fold increased xylitol concentration over those cells grown on glucose as co-substrate. XYL1 overexpressed mutant produced xylitol with a volumetric productivity of 0.83 g/L/h, and a xylitol yield of 98 % xylose. Recombinant yeast strains obtained in this study are promising candidates for xylitol production. This is the first report of XYL1 gene overexpression of C. glycerinogenes WL2002-5 for enhancing the efficiency of xylitol production.

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Acknowledgments

This work was funded by China National “863” High-Tech Program (No. 2012AA021201, No. 2011AA02A207) and supported by the National Natural Science Foundation of China (No. 31270080) and Jiangnan University Independent Scientific Research Programs (No. JUSRP1008, No. JUSRP11431), the Natural Science Foundation of Jiangsu province (No. BK20140134, No. BK20140138) and Six talent peaks project in Jiangsu Province (NO. 2014-XCL-017).

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  1. The Key Lab of Industrial Biotechnology of Ministry of Education, Research Centre of Industrial Microorganisms, School of Biotechnology, Jiangnan University, Wuxi Jiangsu Province, 214122, People’s Republic of China

    Cheng Zhang, Hong Zong, Bin Zhuge, Xinyao Lu, Huiying Fang & Jian Zhuge

  2. Zhejiang Condiments Industry Research Center, Zhejiang Zhengwei Food Co., Ltd., Yiwu, 321000, People’s Republic of China

    Bin Zhuge

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  1. Cheng Zhang
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  2. Hong Zong
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Correspondence to Bin Zhuge.

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Zhang, C., Zong, H., Zhuge, B. et al. Production of Xylitol from d-Xylose by Overexpression of Xylose Reductase in Osmotolerant Yeast Candida glycerinogenes WL2002-5. Appl Biochem Biotechnol 176, 1511–1527 (2015). https://doi.org/10.1007/s12010-015-1661-8

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