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Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM)

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Abstract

In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.

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Acknowledgments

The authors acknowledge the University of Navarra for the funding that supported this project and the Department of Microbiology of The University of Navarra. We also acknowledge the Secretary of State of Investigation, Development and Innovation of the Ministry of Economy and Competitivity of Spain for funding this research within the framework of the SIMcell Project DPI 2012-38090-C03-D3.

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Author notes

  1. A. Zuzuarregui

    Present address: CIC NanoGUNE Consolider, Tolosa Hiribidea 76, 20018, Donostia-San Sebastian, Gipuzkoa, Spain

Authors and Affiliations

  1. CEIT-IK4 and Tecnun, University of Navarra, Paseo de Manuel Lardizábal 15, 20.018, Donostia-San Sebastián, Spain

    E. Pérez-Lorenzo, A. Zuzuarregui, S. Arana & M. Mujika

  2. CIC Microgune, Goiru kalea 9 Polo de Innovación Garaia, 20500, Arrasate-Mondragón, Spain

    E. Pérez-Lorenzo, A. Zuzuarregui, S. Arana & M. Mujika

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  1. E. Pérez-Lorenzo
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  2. A. Zuzuarregui
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  3. S. Arana
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  4. M. Mujika
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Correspondence to E. Pérez-Lorenzo.

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Pérez-Lorenzo, E., Zuzuarregui, A., Arana, S. et al. Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM). Appl Biochem Biotechnol 174, 2492–2503 (2014). https://doi.org/10.1007/s12010-014-1198-2

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  • DOI: https://doi.org/10.1007/s12010-014-1198-2

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