Quartz Crystal Microbalance (QCM)-Based Nanosensors for the Detection of Pathogenic Bacteria

Priyadarshi, Nitesh; Singhal, Nitin Kumar

doi:10.1007/978-981-99-1218-6_7

Nitesh Priyadarshi³ &
Nitin Kumar Singhal³

213 Accesses
1 Citations

Abstract

This book chapter gives an overview of the quartz crystal microbalance sensor (QCM) and how it can be used to detect pathogenic bacteria. The QCM sensor can be assumed as a mechanical transducer for a biosensor device that converts target analytes’ mass into an electrical signal. The detection of microorganisms with a QCM sensor has applications in the environment, biomedical field, and food industry. The characteristics of QCM as a biosensor in the detection of bacterial pathogens are the subject of this book chapter. The chapter explains how QCM is used for bacteria testing and describes the procedures used. Nanoparticles have been widely used in combination with QCM for detection purposes. These nanoparticles are functionalized with different receptor molecules to give the target signal in presence of specific pathogenic bacteria. The use of modified nanoparticles is widely discussed along with various receptor molecules used for bacteria detection. Each receptor molecule results in different sensitivity and offers a range of limit of detection. Apart from its advantage, the limitation and future applications are also discussed.

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Abbreviations

Ab:: Antibody
Ag:: Antigen
Ag-Ab:: Antigen-antibody
Au:: Gold
CFU/mL:: Colony-forming units
HZ:: Hertz
LAMP:: Loop-mediated isothermal amplification
MHZ:: Megahertz
NC:: Negative control
NP:: Nanoparticle
PCR:: Polymerase chain reaction
PIT:: Photochemical immobilization technique
QCM:: Quartz crystal microbalance
QCN:: Quartz crystal nano-balance
SELEX:: Systematic evolution of ligands by exponential enrichment
ssDNA:: Single-stranded DNA
SPR:: Surface plasmon resonance

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National Agri-Food Biotechnology Institute (NABI), Sector-81, S.A.S. Nagar, Mohali, Punjab, India
Nitesh Priyadarshi & Nitin Kumar Singhal

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Nanobiology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India
Amitabha Acharya
National Agri-Food Biotechnology Institute, Mohali, Punjab, India
Nitin Kumar Singhal

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Priyadarshi, N., Singhal, N.K. (2023). Quartz Crystal Microbalance (QCM)-Based Nanosensors for the Detection of Pathogenic Bacteria. In: Acharya, A., Singhal, N.K. (eds) Nanosensors for Point-of-Care Diagnostics of Pathogenic Bacteria. Springer, Singapore. https://doi.org/10.1007/978-981-99-1218-6_7

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DOI: https://doi.org/10.1007/978-981-99-1218-6_7
Published: 16 June 2023
Publisher Name: Springer, Singapore
Print ISBN: 978-981-99-1217-9
Online ISBN: 978-981-99-1218-6
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