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RiHSPRO2, a nematode resistance protein-like homolog from a wild crucifer Rorippa indica (L.) Hiern, is a promising candidate to control mustard aphid Lipaphis erysimi

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Abstract

Nematode resistance protein HSPRO2 of Arabidopsis thaliana has important roles in defence response to the bacterium (Pseudomonas syringae) and shows response against oxidative stress and salicylic acid. We have explored the gene encoding it from Rorippa indica (L.) Hiern, a wild relative of cultivated crucifers experimentally challenged with mustard aphid Lipaphis erysimi. The gene seems to be a strong candidate for aphid tolerance in cultivated mustards. Presently we are reporting the results of a time-course quantitative relative expression analysis of HSPRO2 of R. indica where we have observed ~ tenfold increase in its expression in R. indica at 12 hours post infestation with L. erysimi. We have also isolated the full-length gene (1314 bp) by both 5′ and 3′ RACE (Rapid Amplification of cDNA Ends). Genome walking experiment identified the promoter sequence. Expression, purification and characterisation of RiHSPRO2 showed that it encodes for a 437 amino acid peptide. The 66 kDa recombinant RiHSPRO2 protein was purified for studying its efficacy against L. erysimi in an artificial diet-based insect bioassay that revealed LC50 (Lethal Concentration 50) values of RiHSPRO2 significantly within limits against L. erysimi. The present study might have a significant implication in future towards aphid management program of Brassica juncea through the development of aphid-tolerant transgenic plants.

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Acknowledgements

The present work was supported by Institutional Grant of Bose Institute. The authors acknowledge Prof. Sampa Das for allowing the use of Real-Time PCR Detection System and Dr. Shubho Chaudhuri for providing the pET-32a + vector. We acknowledge the technical support of Mr Jadab K Ghosh. We also acknowledge Ms Susmita Bose for her help in statistical analysis.

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Authors and Affiliations

  1. Division of Plant Biology, Centenary Campus, Bose Institute, Kolkata, West Bengal, India

    Sourav Bose & Samir Ranjan Sikdar

  2. Division of Plant Biology, Main Campus, Bose Institute, Kolkata, West Bengal, India

    Gaurab Gangopadhyay

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  1. Sourav Bose
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Correspondence to Samir Ranjan Sikdar.

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Handling Editor: Joe Louis.

The nucleotide sequence data reported are available in the GenBank database under the accession numbers KY81049, KF431829.

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Bose, S., Gangopadhyay, G. & Sikdar, S.R. RiHSPRO2, a nematode resistance protein-like homolog from a wild crucifer Rorippa indica (L.) Hiern, is a promising candidate to control mustard aphid Lipaphis erysimi. Arthropod-Plant Interactions 12, 701–714 (2018). https://doi.org/10.1007/s11829-018-9615-z

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