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Natural hybridization between transgenic and wild soybean genotypes

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Abstract

Cultivation of transgenic soybean lines [Glycine max (L.) Merr.] and the international trade of their seeds has led to an increased focus on the potential risk of gene flow from transgenic to wild soybean (Glycine soja Sieb. & Zucc.) in countries such as China, Russia, Korea, and Japan, which are the centers of soybean domestication. The degree of natural hybridization between three wild soybean accessions and three transgenic soybean lines that produce recombinant proteins (EGF, IGF-1, and TRX) for use in the skin care industry was estimated under field conditions over 2 years. The wild soybean accessions were sown earlier than the transgenic soybean lines to synchronize the flowering periods. One single hybrid was detected among 16,343 progenies in 2017 and 12 among 333,243 progenies, in 2018. The rate of gene flow from the three transgenic soybean lines to the three wild soybean accessions ranged between 0% and 0.0519% in the 2 years of study. Further studies are required to determine whether the transfer of transgenes (egf, igf-1, and trx) from transgenic to wild soybean alters the ecological fitness of hybrid progenies and the consequences of transgene flow.

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References

  • Burnett MJB, Burnett AC (2019) Therapeutic recombinant protein production in plants: Challenges and opportunities. Plants People Planet 2:121–132

    Article  Google Scholar 

  • Concibido VC, La Vallee B, Mclaird P, Pineda N, Meyer J, Hummel L, Yang J, Wu K, Delannay X (2003) Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivars. Theor Appl Genet 106:576–582

    Article  Google Scholar 

  • Cruz-Reyes R, Ávila-Sakar G, Sánchez-Montoya G, Quesada M (2015) Experimental assessment of gene flow between transgenic squash and a wild relative in the center of origin of cucurbits. Ecosphere 6:248

    Article  Google Scholar 

  • Cunha NB, Murad AM, Cipriano TM, Araújo ACG, Aragão FJL, Leite A, Vianna GR, McPhee TR, Souza GHMF, Waters MJ, Rech EL (2011) Expression of functional recombinant human growth hormone in transgenic soybean seeds. Transgen Res 20:811–826

    Article  CAS  Google Scholar 

  • Delaplane KS, Mayer DF (2000) Crop Pollination by Bees. CABI Publishing, Wallingford

    Book  Google Scholar 

  • Dorokhov D, Ignatov A, Deineko E, Serjapin A, Ala A, Skryabin K (2004) Potential for gene flow from herbicide-resistant GM soybeans to wild soya in the Russian Far East. In: den Nijs HCM, Bartsch ND, Sweet J (eds) Introgression from Genetically Modified Plants into Wild Relatives. CABI Publishing, Wallingford, pp 151–161

    Chapter  Google Scholar 

  • Ellstrand NC, Rieseberg LH (2016) When gene flow really matters: gene flow in applied evolutionary biology. Evol Appl 9:833–836

    Article  PubMed  PubMed Central  Google Scholar 

  • Ertl DS, Fehr WR (1985) Agronomic performance of soybean genotypes from Glycine max × Glycine soja crosses. Crop Sci 25:589–592

    Article  Google Scholar 

  • Fehr WR (1980) Soybean. In: Fehr WR, Hadley HH (eds) Hybridization of Crop Plants. American Society of Agronomy-Crop Science Society of America, Madison, pp 589–599

    Chapter  Google Scholar 

  • Fukuda Y (1933) Cyto-genetical studies on the wild and cultivated Manchurian soy beans (Glycine L.). Jap J Bot 6:489–506

    Google Scholar 

  • Gepts P, Papa R (2003) Possible effects of (trans)gene flow from crops on the genetic diversity from landraces and wild relatives. Environ Biosaf Res 2:89–103

    Article  Google Scholar 

  • Guan ZJ, Zhang PF, Wei W, Mi XC, Kang DM, Liu B (2015) Performance of hybrid progeny formed between genetically modified herbicide-tolerant soybean and its wild ancestor. AoB PLANTS 7:plv121

    Article  PubMed  PubMed Central  Google Scholar 

  • Hur SN (1978) Studies on the physical and ecological characteristics of wild soybean (Glycine soja). II. Growth characteristics of Glycine soja in the habitat. J Kor Soc Grassl Sci 1:29–33

    Google Scholar 

  • Hwang TY, Gwak BS, Sung J, Kim HS (2020) Genetic diversity patterns and discrimination of 172 Korean soybean (Glycine max (L.) Merrill) varieties based on SSR analysis. Agriculture 10:77

    Article  Google Scholar 

  • Jenczewski E, Ronfort J, Chèvre AM (2003) Crop-to-wild gene flow, introgression and possible fitness effects of transgenes. Environ Biosaf Res 2:9–24

    Article  Google Scholar 

  • Kaga A, Tomooka N, Phuntsho U, Kuroda Y, Kobayashi N, Isemura T, Gilda MJ, Vaughan DA (2005) Exploration and collection for hybrid derivatives between wild and cultivated soybean: Preliminary survey in Akita and Hiroshima Prefectures, Japan. AREIPGR 21:59–71

    Google Scholar 

  • Karasawa K (1936) Crossing experiments with Glycine soja and G. ussuriensis. Jap J Bot 8:113–118

    Google Scholar 

  • Kiang YT, Chiang YC, Kaizuma N (1992) Genetic diversity in natural populations of wild soybean in Iwate Prefecture, Japan. J Hered 83:325–329

    Article  Google Scholar 

  • Kim KU, Kang TD, Lee JH, Lee IJ, Shin DH, Hwang YH, Kim SU, Kim HM (2003) Physio-ecological characteristics of wild soybeans (Glycine soja) collected throughout Korea and their response to glyphosate. Kor J Weed Sci 23:153–159

    Google Scholar 

  • Kim HJ, Kim DY, Moon YS, Pack IS, Park KW, Chung YS, Kim YJ, Nam KH, Kim CG (2019) Gene flow from herbicide resistant transgenic soybean to conventional soybean and wild soybean. Appl Biol Chem 62:54

    Article  Google Scholar 

  • Kim DY, Eom MS, Kim HJ, Ko EM, Pack IS, Park JH, Park KW, Nam KH, Oh SD, Kim JK, Seo JS, Kim CG (2020) Gene flow from transgenic soybean, developed to obtain recombinant proteins for use in the skin care industry, to non-transgenic soybean. Appl Biol Chem 63:65

    Article  CAS  Google Scholar 

  • Kofsky J, Zhang H, Song BH (2018) The untapped genetic reservoir: The past, current, and future applications of the wild soybean (Glycine soja). Front Plant Sci 9:949

    Article  PubMed  PubMed Central  Google Scholar 

  • Komarov VL, Shishkin BK, Bobrov EG (1972) Flora of the U.S.S.R. In: Leguminosae: Oxytropis, Hedysarum, vol 13. Israel Program from Scientific Translations, Jerusalem

  • Kubo A, Aono M, Nakajima N, Nishizawa T, Tamaoki M, Saji H (2013) Characterization of hybrids between wild and genetically modified glyphosate-tolerant soybeans. Plant Biotechnol 30:335–345

    Article  CAS  Google Scholar 

  • Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Akiyama H, Goda Y, Toyoda M, Hino A (2002) Novel reference molecules for quantitation of genetically modified maize and soybean. J AOAC Int 85:1077–1089

    Article  CAS  PubMed  Google Scholar 

  • Kuroda Y, Kaga A, Apa A, Vaughan DA, Tomooka N, Yano H, Matsuoka N (2005) Exploration, collection and monitoring of wild soybean and hybrid derivatives between wild soybean and cultivated soybean: Based on field surveys at Akita, Ibaraki, Aichi, Hiroshima and Saga Prefectures. AREIPGR 21:73–95

    Google Scholar 

  • Kuroda Y, Kaga A, Tomooka N, Vaughan D (2010) The origin and fate of morphological intermediates between wild and cultivated soybeans in their natural habitats in Japan. Mol Ecol 19:2346–2360

    Article  CAS  PubMed  Google Scholar 

  • Kuroda Y, Kaga A, Tomooka N, Yano H, Takada Y, Kato S, Vaughan D (2013) QTL affecting fitness of hybrids between wild and cultivated soybeans in experimental fields. Ecol Evol 3:2150–2168

    Article  PubMed  PubMed Central  Google Scholar 

  • LeRoy AR, Fehr WR, Cianzio SR (1991) Introgression of genes for small seed size from Glycine soja into G. max. Crop Sci 31:693–697

    Article  Google Scholar 

  • Li F, Liu X, Wu S, Luo Q, Yu B (2019) Hybrid identification for Glycine max and Glycine soja with SSR markers and analysis of salt tolerance. PeerJ 7:e6483

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu J, Zhou B, Yang C, Li Y, Jiang L, Zhang M, Tao B, Qiu L (2012) Gene flowing of genetically modified glyphosate-resistant soybean with EPSPS. Soybean Sci 31:517–521

    CAS  Google Scholar 

  • Lu BR (2004) Conserving biodiversity of soybean gene pool in the biotechnology era. Plant Spec Biol 19:115–125

    Article  Google Scholar 

  • Mizuguti A, Yoshimura Y, Matsuo K (2009) Flowering phenologies and natural hybridization of genetically modified and wild soybeans under field conditions. Weed Biol Manag 9:93–96

    Article  Google Scholar 

  • Mizuguti A, Ohigashi K, Yoshimura Y, Kaga A, Kuroda Y, Matsuo K (2010) Hybridization between GM soybean (Glycine max (L.) Merr.) and wild soybean (Glycine soja Sieb. et Zucc.) under field conditions in Japan. Environ Biosaf Res 9:13–23

    Article  Google Scholar 

  • Monasterolo M, Musicante ML, Valladares GR, Salvo A (2015) Soybean crops may benefit from forest pollinators. Agric Ecosyst Environ 202:217–222

    Article  Google Scholar 

  • Moon KB, Park JS, Park YI, Song IJ, Lee HJ, Cho HS, Jeon JH, Kim HS (2020) Development of systems for the production of plant-derived biopharmaceuticals. Plants 9:30

    Article  CAS  Google Scholar 

  • Nakayama Y, Yamaguchi H (2002) Natural hybridization in wild soybean (Glycine max ssp. soja) by pollen flow from cultivated soybean (Glycine max ssp. max) in a designed population. Weed Biol Manage 2:25–30

    Article  Google Scholar 

  • Nedoluzhko AV, Dorokhov DB (2007) Study of the biosafety of genetically modified soybean in the center of its origin and diversity in the Far East of the Russian Federation. Cytol Genet 41:190–198

    Article  Google Scholar 

  • O’Keefe BR, Murad AM, Vianna GR, Ramessar K, Saucedo CJ, Wilson J, Buckheit KW, da Cunha NB, Araújo ACG, Lacorte CC, Madeira L, McMahon JB, Rech EL (2015) Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV. Plant Biotechnol J 13:884–892

    Article  PubMed  PubMed Central  Google Scholar 

  • Oh SD, Jang YJ, Park SY, Suh SJ, Lee B (2020) Characterization of hybrid soybean seeds between β-carotene enhanced transgenic soybean and wild soybean. Weed Turfgrass Sci 9:129–139

    Google Scholar 

  • Ohara M, Shimamoto Y (1994) Some ecological and demographic characteristics of two growth forms of wild soybean (Glycine soja). Can J Bot 72:486–492

    Article  Google Scholar 

  • Ohara M, Shimamoto Y, Sanbuichi T (1989) Distribution and ecological features of wild soybeans (Glycine soja Sieb. et Zucc.) in Hokkaido. J Fac Agric Hokkaido Univ 64:43–50

    Google Scholar 

  • Palmer RG, Heer HE (1984) Agronomic characteristics and genetics of a chromosome interchange in soybean. Euphytica 33:651–663

    Article  Google Scholar 

  • Schillberg S, Raven N, Spiegel H, Rasche S, Buntru M (2019) Critical analysis of the commercial potential of plants for the production of recombinant proteins. Front Plant Sci 10:720

    Article  PubMed  PubMed Central  Google Scholar 

  • Scurrah M, Celis-Gamboa C, Chumbiauca S, Salas A, Visser RGF (2008) Hybridization between wild and cultivated potato species in the Peruvian Andes and biosafety implications for deployment of GM potatoes. Euphytica 164:881–892

    Article  Google Scholar 

  • Singh RJ, Hymowitz T (1999) Soybean genetic resources and crop improvement. Genome 42:605–616

    Article  CAS  Google Scholar 

  • Ting CL (1946) Genetic studies on the wild and cultivated soybeans. J Am Soc Agron 38:381–393

    Article  Google Scholar 

  • Wang KJ, Li XH (2011) Interspecific gene flow and the origin of semi-wild soybean revealed by capturing the natural occurrence of introgression between wild and cultivated soybean populations. Plant Breed 130:117–127

    Article  Google Scholar 

  • Wang KJ, Li XH (2013) Pollen dispersal of cultivated soybean into wild soybean under natural conditions. Crop Sci 53:2497–2505

    Article  Google Scholar 

  • Xu J, Towler M, Weathers PJ (2018) Platforms for plant-based protein production. In: Pavlov A, Bley T (eds) Bioprocessing of Plant In Vitro Systems. Springer International Publishing AG, Cham, Switzerland, pp 509–548

    Chapter  Google Scholar 

  • Yoon MS, Lee J, Baek HJ, Cho GT, Kim CY, Cho YH, Kim TS, Cho EG (2007) SSR profiling and its variation in soybean germplasm. Kor J Crop Sci 52:81–88

    Google Scholar 

  • Yuk MJ, Park HR, Zhang CJ, Lim SH, Jeong SC, Chung YS, Kim DS (2021) Environmental risk assessment of glufosinate-resistant soybean by pollen-mediated gene flow under field conditions in the region of the genetic origin. Sci Total Environ 762:143073

    Article  Google Scholar 

  • Zhang J, Sun H, Zhao L, Zhang C, Yan H, Peng B, Li W (2018) Nectar secretion of RN-type cytoplasmic male sterility three lines in soybean [Glycine max (L.) Merr.]. J Integr Agric 17:1085–1092

    Article  Google Scholar 

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Acknowledgements

This research was funded by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM5362113) and the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01368601), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Republic of Korea

    Do Young Kim, Jin Ho Heo, In Soon Pack, Jung-Ho Park, Min Shik Um, Hye Jin Kim & Chang-Gi Kim

  2. Department of Crop Science, Chungnam National University, Daejeon, 34134, Republic of Korea

    Min Shik Um, Hye Jin Kim & Kee Woong Park

  3. LMO Research Team, National Institute of Ecology, Seocheon, 33657, Republic of Korea

    Kyong-Hee Nam

  4. Biosafety Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, Republic of Korea

    Sung Duk Oh

  5. Division of Life Sciences, Incheon National University, Incheon, 22012, Republic of Korea

    Jae Kwang Kim

  6. CELLTRION, Incheon, 22014, Republic of Korea

    Ju Seok Seo

  7. Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju, 28159, Republic of Korea

    Hye Jin Kim

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  1. Do Young Kim
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Kim, D.Y., Heo, J.H., Pack, I.S. et al. Natural hybridization between transgenic and wild soybean genotypes. Plant Biotechnol Rep 15, 299–308 (2021). https://doi.org/10.1007/s11816-021-00685-2

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