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Preparation and enzymatic activity of Fe3O4-IDA-Ni/NAD kinase magnetic catalyst

  • Biotechnology
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Abstract

The use of oxidoreductases as biocatalysts for industrial production of valuable compounds has a strong demand for NADP. Herein, we prepared superparamagnetic NAD kinase catalyst to synthesize NADP in vitro. First, Fe3O4 particles were synthesized through a solvothermal method, followed by the chemical modification with epichloro-hydrin, iminodiacetic acid, and Ni2+ to yield functional Fe3O4 sub-microspheres. Subsequently, NAD kinase of Escherichia coli was overexpressed and immobilized on to the surface of magnetic sub-microspheres. The immobilized NAD kinase was used to catalyze the conversion of NAD to NADP in a cell-free system. Under optimal condition, the conversion ratio of NAD reached 91.7% and remained at 86.3% after repeated use for five times. Our study revealed that the novel magnetic NAD kinase catalyst possessed favorable properties for magnetic manipulation and NADP production.

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Acknowledgements

This work was supported by Industry-University-Research Collaborative Innovation and Scientific and Technical Cooperation project of Xiamen Municipal Bureau of Science and Technology (3502Z20172020), Grants from National Natural Science Foundation of China (31870075), and the Fundamental Research Funds for the Central Universities (XK1802-8, PT1904). The authors declare that they have no competing financial interests.

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Changxia Liu, Yadi Yang, Huafeng Gao, Xiaoshuang Bai & Zheng-Jun Li

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  1. Changxia Liu
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  2. Yadi Yang
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  3. Huafeng Gao
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  4. Xiaoshuang Bai
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  5. Zheng-Jun Li
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Correspondence to Changxia Liu or Zheng-Jun Li.

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Liu, C., Yang, Y., Gao, H. et al. Preparation and enzymatic activity of Fe3O4-IDA-Ni/NAD kinase magnetic catalyst. Korean J. Chem. Eng. 37, 475–481 (2020). https://doi.org/10.1007/s11814-019-0472-y

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  • DOI: https://doi.org/10.1007/s11814-019-0472-y

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