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Recent trends and novel concepts in cofactor-dependent biotransformations

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Abstract

Cofactor-dependent enzymes catalyze a broad range of synthetically useful transformations. However, the cofactor requirement also poses economic and practical challenges for the application of these biocatalysts. For three decades, considerable research effort has been devoted to the development of reliable in situ regeneration methods for the most commonly employed cofactors, particularly NADH and NADPH. Today, researchers can choose from a plethora of options, and oxidoreductases are routinely employed even on industrial scale. Nevertheless, more efficient cofactor regeneration methods are still being developed, with the aim of achieving better atom economy, simpler reaction setups, and higher productivities. Besides, cofactor dependence has been recognized as an opportunity to confer novel reactivity upon enzymes by engineering their cofactors, and to couple (redox) biotransformations in multi-enzyme cascade systems. These novel concepts will help to further establish cofactor-dependent biotransformations as an attractive option for the synthesis of biologically active compounds, chiral building blocks, and bio-based platform molecules.

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Acknowledgments

JHS thanks the Austrian Science Fund (FWF) for financial support in the form of an “Erwin Schrödinger” fellowship (J3244-N17).

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Authors and Affiliations

  1. Institute of Microbiology, Chair of Molecular Biotechnology, Technische Universität Dresden, 01062, Dresden, Germany

    Selin Kara & Marion B. Ansorge-Schumacher

  2. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628BL, Delft, The Netherlands

    Joerg H. Schrittwieser & Frank Hollmann

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  1. Selin Kara
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  3. Frank Hollmann
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  4. Marion B. Ansorge-Schumacher
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Corresponding authors

Correspondence to Selin Kara or Marion B. Ansorge-Schumacher.

Additional information

S. Kara and J. H. Schrittwieser contributed equally to this work

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Kara, S., Schrittwieser, J.H., Hollmann, F. et al. Recent trends and novel concepts in cofactor-dependent biotransformations. Appl Microbiol Biotechnol 98, 1517–1529 (2014). https://doi.org/10.1007/s00253-013-5441-5

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  • DOI: https://doi.org/10.1007/s00253-013-5441-5

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