Abstract
The impact of dissolved oxygen (DO) at aerobic phase on the nitrogen removal, extracellular polymeric substances (EPS), microbial activity and microbial community of sequencing batch biofilm reactor (SBBR) have been evaluated in treating mariculture wastewater. The oxygen uptake rate and nitrification rate declined with DO concentration from 3–4 to 1–1.5 mg L−1, whereas the denitrification rate had an increment. The activities of nitrifying enzymes reduced with the decrease of DO concentration at aerobic phase, but those of denitrifying enzymes illustrated opposite results. The nitrification and denitrification rates displayed the similar variation tendency with the relevant enzymatic activities as DO concentration decreased. The protein (PN) and polysaccharide (PS) content in EPS decreased as DO concentration declined, whereas the PN/PS ratio increased. The microbial community showed obvious difference as DO concentration decreased from 3–4 to 1–1.5 mgL−1. The microbial co-occurrence, keystone taxa and significant difference illustrated some variations at different DO concentrations.
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References
APHA, 2005. Standard Methods for the Examination of Water and Wastewater. 21st ed. American Public Health Association, Washington, D. C., USA, 1–22.
Banerjee, S., Kirkby, C. A., Schmutter, D., Bissett, A., Kirkegaard, J. A., and Richardson, A. E., 2016. Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biology and Biochemistry, 97: 188–198.
Berry, D., and Widder, S., 2014. Deciphering microbial interactions and detecting keystone species with co-occurrence networks. Frontiers in Microbiology, 5: 219.
Davidson, J., Helwig, N., and Summerfelt, S. T., 2008. Fluidized sand biofilters used to remove ammonia, biochemical oxygen demand, total coliform bacteria, and suspended solids from an intensive aquaculture effluent. Aquacultural Engineering, 39: 6–15.
Eding, E. H., Kamstra, A., Verreth, J. A. J., Huisman, E. A., and Klapwijk, A., 2006. Design and operation of nitrifying trickling filters in recirculating aquaculture: A review. Aquacultural Engineering, 34: 234–260.
Fahrbach, M., Kuever, J., Meinke, R., Kämpfer, P., and Hollender, J., 2006. Denitratisoma oestradiolicum gen. nov., sp. nov., a 17β-oestradiol-degrading, denitrifying betaproteobacterium. International Journal of Systematic and Evolutionary Microbiology, 56(7): 1547–1552.
FAO, 2020. The State of World Fisheries and Aquaculture. Food and Agriculture Organization of the United Nations.
Faust, K., and Raes, J., 2012. Microbial interactions: from networks to models. Nature Reviews Microbiology, 10(8): 538–550.
Fontenot, Q., Bonvillain, C., Kilgen, M., and Boopathy, R., 2007. Effects of temperature, salinity, and carbon: Nitrogen ratio on sequencing batch reactor treating shrimp aquaculture wastewater. Bioresource Technology, 98: 1700–1703.
Gao, F., Li, Z. W., Chang, Q. B., Gao, M. C., She, Z. L., Wu, J., et al., 2018. Effect of florfenicol on performance and microbial community of a sequencing batch biofilm reactor treating mariculture wastewater. Environmental Technology, 39(3): 363–372.
Gao, Y., Li, Y., Ren, L., Sha, M. Q., Lv, D. Q., Wang, S., et al, 2021. Bio-clogging mitigation in vertical subsur- face flow constructed wetlands using rhamnolipids-citric acid compound. Chemical Engineering Journal, 426: 131278.
Gao, Y. F., Zhang, C. S., Rong, H. W., Zheng, G. L., and Zhao, L. M., 2017. The effect of dissolved oxygen concentration (DO) on oxygen diffusion and bacterial community structure in moving bed sequencing batch reactor (MBSBR). Water Research, 108: 86–94.
Grab, R., Avnimelech, Y., Defoirdt, T., Bossier, P., and Verstraete, W., 2007. Nitrogen removal techniques in aquaculture for a sustainable production. Aquaculture, 270: 1–14.
Guo, H. H., Gu, J., Wang, X. J., Song, Z. L., Qian, X., Sun, W., et al., 2021. Negative effects of oxytetracycline and copper on nitrogen metabolism in an aerobic fermentation system: Characteristics and mechanisms. Journal of Hazardous Materials, 403: 123890.
Hu, T. L., and Kung, K. T., 2000. Study of heterotrophic nitrifying bacteria from wastewater treatment systems treating acrylonitrile, butadiene and styrene resin wastewater. Water Science and Technology, 42(3–4): 315–321.
Huang, F., Pan, L. Q., He, Z. Y., Zhang, M. Y., and Zhang, M. Z., 2021. Heterotrophic nitrification-aerobic denitrification characteristics and antibiotic resistance of two bacterial consortia from Marinomonas and Halomonas with effective nitrogen removal in mariculture wastewater. Journal of Environmental Management, 279: 111786.
Huang, Z. J., Kong, F. L., Li, Y., Xu, G. M., and Wang, S., 2020. Advanced treatment of effluent from municipal wastewater treatment plant by strengthened ecological floating bed. Bioresource Technology, 309: 123358.
Kaye, J. Z., Marquez, M. C., Ventosa, A., and Baross, J. A., 2004. Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: Halophilic bacteria isolated from deep-sea hydrothermal-vent environments. International Journal of Systematic and Evolutionary Microbiology, 54: 499–511.
Liang, Y. X., Zhu, H., Banuelos, G., Yan, B. X., Zhou, Q. W., Yu, X. F., et al., 2017. Constructed wetlands for saline wastewater treatment: A review. Ecological Engineering, 98: 275–285.
Liu, S. L., Daigger, G. T., Liu, B. T., Zhao, W. Y., and Liu, J., 2020. Enhanced performance of simultaneous carbon, nitrogen and phosphorus removal from municipal wastewater in an anaerobic-aerobic-anoxic sequencing batch reactor (AOA-SBR) system by alternating the cycle times. Bioresource Technology, 301: 122750.
Miao, L., Zhang, Q., Wang, S. Y., Li, B. K., Wang, Z., Zhang, S. J., et al., 2018. Characterization of EPS compositions and microbial community in an Anammox SBBR system treating landfill leachate. Bioresource Technology, 249: 108–116.
Miao, Y., Wang, Z., Liao, R. H., Shi, P., and Li, A. M., 2017. Assessment of phenol effect on microbial community structure and function in an anaerobic denitrifying process treating high concentration nitrate wastewater. Chemical Engineering Journal, 330: 757–763.
Pugazhendi, A., Alreeshi, G. G., Jamal, M. T., Karuppiah, T., and Jeyakumar, R. B., 2021. Bioenergy production and treatment of aquaculture wastewater using saline anode microbial fuel cell under saline condition. Environmental Technology and Innovation, 21: 101331.
Sheng, G. P., Yu, H. Q., and Li, X. Y., 2010. Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: A review. Biotechnology Advances, 28: 882–894.
Song, C. G., Zhao, C. K., Wang, Q. Z., Lu, S. L., She, Z. L., Zhao, Y. G., et al., 2021. Impact of carbon/nitrogen ratio on the performance and microbial community of sequencing batch biofilm reactor treating synthetic mariculture wastewater. Journal of Environmental Management, 298: 113528.
Sun, J. Q., Liu, L. F., and Yang, F. L., 2020. Successful bioelectrochemical treatment of nitrogenous mariculture waste-water by enhancing nitrogen removal via synergy of algae and cathodic photo-electro-catalysis. Science of the Total Environment, 743: 140738.
Sun, L., Zhao, X. X., Zhang, H. F., and Zhang, Y. Q., 2015. Biological characteristics of a denitrifying phosphorus-accumulating bacterium. Ecological Engineering, 81: 82–88.
Trubitsyn, I. V., Belousova, E. V., Tutukina, M. N., Merkel, A. Y., Dubinina, G. A., and Grabovich, M. Y., 2014. Expansion of ability of denitrification within the filamentous colorless sulfur bacteria of the genus Thiothrix. FEMS Microbiology Letters, 358(1): 72–80.
Wang, J. Y., Rong, H. W., and Zhang, C. S., 2018. Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor. Journal of Bioscience and Bioengineering, 125(5): 532–542.
Wang, S., Gao, M. C., She, Z. L., Zheng, D., Jin, C. J., Guo, L., et al., 2016. Long-term effects of ZnO nanoparticles on nitrogen and phosphorus removal, microbial activity and microbial community of a sequencing batch reactor. Bioresource Technology, 216: 428–436.
Wang, X. C., Shen, J. M., Kang, J., Zhao, X., and Chen, Z. L., 2019. Mechanism of oxytetracycline removal by aerobic granular sludge in SBR. Water Research, 161: 308–318.
Ya, T., Du, S., Li, Z. Y., Liu, S. D., Zhu, M. H., Liu, X. J., et al, 2021. Successional dynamics of molecular ecological network of anammox microbial communities under elevated salinity. Water Research, 188: 116540.
Yan, L. L., Liu, Y., Wen, Y., Ren, Y., Hao, G. X., and Zhang, Y., 2015. Role and significance of extracellular polymeric substances from granular sludge for simultaneous removal of organic matter and ammonia nitrogen. Bioresource Technology, 179: 460–466.
Yuan, M. M., Guo, X., Wu, L. W., Zhang, Y., Xiao, N. J., Ning, D. L., et al., 2021. Climate warming enhances microbial network complexity and stability. Nature Climate Change, 11: 343–348.
Zhao, X., Yang, Y., Feng, K., Wang, X. H., Liu, B. F., Xie, G. J., et al., 2021. Self-regulating microbiome networks ensure functional resilience of biofilms in sand biofilters during manganese load fluctuations. Water Research, 188: 116473.
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The work was supported by the National Natural Science Foundation of China (No. 52070172), and the Fundamental Research Funds for the Central Universities (No. 201964003).
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Huo, S., Liu, W., Zhao, C. et al. Performance Evaluation and Microbial Shift of Sequencing Batch Biofilm Reactor Treating Synthetic Mariculture Wastewater Under Different Dissolved Oxygen at Aerobic Phase. J. Ocean Univ. China 22, 1692–1701 (2023). https://doi.org/10.1007/s11802-023-5450-7
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DOI: https://doi.org/10.1007/s11802-023-5450-7