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Extraction of Protease Produced by Sea Mud Bacteria and Evaluation of Antifouling Performance

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Abstract

Enzyme-based antifouling coatings are potential alternative to traditional tributyltin-based coatings in the marine bio-fouling control depended on its environmental friendliness. Proteases are usually the key antifouling active components in enzyme based antifouling coatings. In this work, based on biological antagonism effect, a marine proteolytic bacterial strain of Bacillus velezensis was isolated from the sea mud, and denoted as SM-1. The scanning electron microscope (SEM) revealed that the bacteria are rod-shaped with length 1-1.3 μm. The protease-producing conditions of SM-1 were investigated, and it was found that the culture solution displays higher proteolytic activity under the culture conditions of 35°C, 10‰-20‰ salinity, pH 6–9 and more than 7d culture time. Moreover, the 25kDa protein was confirmed to be the main active component in the crude protease, which was revealed via the experiment of SDS-PAGE. The antifouling assay indicated that the protease SM-1 has remarkable effect on the decomposition of barnacle cement and diatom secretion, and also can effectively inhibit the attachment of barnacle cyprids, diatom and mussel byssus. Therefore, this protease potentially can be used as environmental-friendly antifoulant of enzyme-based marine antifouling coatings, and this work also provides a new approach to obtain antifouling protease via isolating proteolytic bacteria from the sea mud surrounding representative fouling organisms.

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Acknowledgments

This work was supported by the National Basic Research Program of China (No. 2014CB643305) and the National Natural Science Foundation of China (No. 213 01161).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China

    Li Wang & Liangmin Yu

  2. State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute, Qingdao, 266101, China

    Li Wang & Cunguo Lin

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  1. Li Wang
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  2. Liangmin Yu
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  3. Cunguo Lin
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Correspondence to Cunguo Lin.

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Wang, L., Yu, L. & Lin, C. Extraction of Protease Produced by Sea Mud Bacteria and Evaluation of Antifouling Performance. J. Ocean Univ. China 18, 1139–1146 (2019). https://doi.org/10.1007/s11802-019-3843-4

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  • DOI: https://doi.org/10.1007/s11802-019-3843-4

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