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Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen

PEG-2000 液氮保藏混合浸矿菌对黄铜矿生物浸出的影响

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Abstract

A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed.

摘要

首先, 富集到具有浸矿能力的混合培养物, 然后, 以 PEG-2000 作为保护剂, 对混合培养物液氮冷冻保藏 6 个月和 12 个月, 对其保藏前、 后黄铜矿的浸出能力、 活性和多样性进行比较. 结果表明, 当用原始培养物浸出黄铜矿时, 20 天内铜的浸出率为 95.7%. 冷冻保藏 6 个月后, 混合培养物的铜浸出率在 25 天内为 94.9%, 保藏 12 个月后, 在 35 天内为 93.6%. 保藏 6 个月和 12 个月后, 混合菌种的活性分别达到 87%和 41%. 此外, 对原始培养物进行生态学分析, 鉴定得到 Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans 和 Pseudomonas aeruginosa. 冷冻保藏 12 个月后, 混合培养物的群落组成发生了变化, 但优势菌群依然存在.

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China

    Xue-ling Wu  (吴学玲), Xiao-yan Wu  (吴晓燕), Fan-fan Deng  (邓凡凡), Li Shen  (申丽), Jiao-kun Li  (李交昆), Tang-jian Peng  (彭堂见), Run-lan Yu  (余润兰), Yuan-dong Liu  (刘元东), Guan-zhou Qiu  (邱冠周) & Wei-min Zeng  (曾伟民)

  2. Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China

    Xue-ling Wu  (吴学玲), Li Shen  (申丽), Jiao-kun Li  (李交昆), Tang-jian Peng  (彭堂见), Run-lan Yu  (余润兰), Yuan-dong Liu  (刘元东), Guan-zhou Qiu  (邱冠周) & Wei-min Zeng  (曾伟民)

  3. CSIRO Process Science and Engineering Clayton, Victoria, 3169, Australia

    Miao Chen  (陈淼)

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  1. Xue-ling Wu  (吴学玲)
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Correspondence to Wei-min Zeng  (曾伟民).

Additional information

Foundation item: Projects(31470230, 51320105006, 51604308) supported by the National Natural Science Foundation of China; Project(2017RS3003) supported by the Youth Talent Foundation of Hunan Province of China; Project(2018JJ2486) supported by the Natural Science Foundation of Hunan Province of China; Project(2018WK2012) supported by the Key Research and Development Projects in Hunan Province, China; Project(2018zzts767) supported by the Fundamental Research Funds for the Central Universities, China

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Wu, Xl., Wu, Xy., Deng, Ff. et al. Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen. J. Cent. South Univ. 27, 1386–1394 (2020). https://doi.org/10.1007/s11771-020-4374-2

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