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Effects of single-walled carbon nanotubes on growth and physiological characteristics of Microcystis aeruginosa

单壁碳纳米管对铜绿微囊藻生长及生理特性的影响

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Abstract

In order to explore a novel and potential method using carbon nanotubes (CNTs) for controlling blue-green algal blooms efficiently in future, effects of single-walled carbon nanotubes (SWCNTs) on Microcystis aeruginosa growth control were investigated under lab cultured conditions. Related physiological changes were tested involving several important enzyme of antioxidant defense system (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondiadehyde (MDA), photosynthetic pigments, protein, soluble sugar and extracellular microcystin toxins (MC-LR)). Algal cell density was significantly inhibited by SWCNTs at high concentration (>5.00 mg/L), and the inhibition rate was dose-dependent. For treatment with 100 mg/L SWCNTs, the inhibitory rates even reached above 90%. 96 h IC50 was determined as 22 mg/L. Antioxidant enzyme activities were dramatically dropped with increasing lipid peroxidation at higher SWCNTs concentration, indicating intracellular generation of reactive oxygen species (ROS) and oxidative stress damage in algae. Reduction of photosynthetic pigments, soluble sugar and protein contents suggested that SWCNTs may severely ruin algal photosynthesis system, destroy the metabolism-related structure of cell, and thus lead to negative physiological status in M. aeruginosa. Besides, SWCNTs can effectively decrease the amount of extracellular microcystins in culture medium.

摘要

为了探索一种潜在新型的使用碳纳米管(CNTs)高效控制水华蓝藻的处理方法, 本研究调查 了在实验室条件下单壁碳纳米管(SWCNTs)对铜绿微囊藻(Microcystis aeruginosa)的生长及控制的影 响。相关生理变化检测包括重要的抗氧化酶, 如超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化 氢酶(CAT)、丙二醛(MDA), 有光合色素、蛋白、可溶性糖和胞外藻毒素(MC-LR)。藻细胞密度在高 浓度SWCNTs (>5.00 mg/L)处理时受到明显抑制, 抑制率具有浓度依赖性。当处理100 mg/L SWCNTs 时, 抑制率可达90%以上。96 h IC50 为22 mg/L。在高浓度SWCNTs 处理时, 抗氧化酶活性明显下 降, 脂质过氧化物上升, 表明胞内产生了活性氧自由基(ROS)和氧化胁迫伤害。光合色素、可溶性糖 和蛋白含量下降, 表明SWCNTs 可能摧毁了藻的光和系统, 细胞的相关代谢结构, 导致了Microcystis aeruginosa 糟糕的生理状态。此外, SWCNTs 还可以有效地降低培养基中的微囊藻毒素。

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Authors and Affiliations

  1. Department of Environmental Engineering, College of Environment, Sichuan Agricultural University, Chengdu, 611130, China

    Yang Wu  (武阳), Ying-jun Wang  (王应军), Yuan-wei Li  (李远伟) & Jin-ge Du  (杜金戈)

  2. Department of Environmental Engineering, School of Energy and Environment, Southeast University, Nanjing, 210009, China

    Zhang-hong Wang  (王章鸿)

  3. Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, 611130, China

    Shi-huai Deng  (邓仕槐)

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  1. Yang Wu  (武阳)
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Correspondence to Ying-jun Wang  (王应军).

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Foundation item: Project(035703011) supported by the Scientific Research Double Support Program of SICAU, China

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Wu, Y., Wang, Yj., Li, Yw. et al. Effects of single-walled carbon nanotubes on growth and physiological characteristics of Microcystis aeruginosa. J. Cent. South Univ. 25, 1628–1641 (2018). https://doi.org/10.1007/s11771-018-3855-z

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