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Aspergillus oryzae Lipase-Catalyzed Synthesis of Dioleoyl; Palmitoyl-Rich Triacylglycerols in Two Reactors

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

Dioleoyl; palmitoyl-rich triacylglycerols (OPO-rich TAG) were synthesized through Aspergillus oryzae lipase (AOL)-catalyzed acidolysis of palm stearin with commercial oleic acid by a one-step process in a stirred tank reactor and continuous packed bed reactor to evaluate the feasibility of using immobilized AOL. AOL was found to be valuable for the synthesis of OPO-rich TAG when compared with commercial lipase from Thermomyces lanuginose (Lipozyme® TL IM; Novozymes A/S, Bagsvaerd, Denmark). The C52 (triglycerides with a carbon number of 52, stands for OPO, OPL, LPL and their isomers) content of AOL was higher (45.65 %), and the intensity of treatment (IOT: lipase amount × reaction time/TAG amount) of AOL was just 6.25 % of that of Lipozyme® TL IM under similar reaction conditions in the stirred tank reactor. Response surface methodology were used to optimize the reaction conditions of the AOL-catalyzed acidolysis is reaction in the packed bed reactor. The optimal point for the set of experimental conditions generated were as follows: residence time 3.0 h; temperature 62.09 °C; substrate molar ratio 7.13 mol/mol. The highest C52 content obtained was 48.60 ± 2.36 %, with 57.46 ± 1.72 % total palmitic acid at the sn-2 position and 74.21 ± 2.45 % oleic acid at the sn-1,3 positions. The half-life of AOL was 24 h in the stirred tank reactor and 140 h in the packed bed reactor. The immobilized AOL achieved similar conversion and selectivity to commercial lipases for the catalyzed synthesis of OPO-rich TAG and may offer a cheaper alternative.

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Acknowledgments

This research was financially supported by the Science & Technology Major Project of Zhejiang Province, China (No. 2012C12005-2), and the 863 project (2013AA102207).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, People’s Republic of China

    Siyun Gu & Rongrong Lu

  2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Fengqin Feng, Yang Li & Wei Wei

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  1. Siyun Gu
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  2. Fengqin Feng
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  3. Yang Li
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  4. Wei Wei
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Correspondence to Rongrong Lu.

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Gu, S., Feng, F., Li, Y. et al. Aspergillus oryzae Lipase-Catalyzed Synthesis of Dioleoyl; Palmitoyl-Rich Triacylglycerols in Two Reactors. J Am Oil Chem Soc 93, 1347–1354 (2016). https://doi.org/10.1007/s11746-016-2887-5

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  • DOI: https://doi.org/10.1007/s11746-016-2887-5

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