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Morphological characterization and assessment of genetic diversity of natural Moroccan populations of Capparis spinosa

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Abstract

Capparis spinosa is one of the most important species in the genus Capparis owing to its ecological, medicinal and economic values. It spread in the North of Africa and grows naturally in Morocco. In this work, we evaluate the inter-variation, in eight natural Moroccan populations of Capparis spp. from the Fez-Meknes region in north-central Morocco, at the morphological and genetic level. Genetic variation of this species was assessed using Inter-Simple Sequence Repeats (ISSR) markers. The morphological traits submitted to canonical discriminant analysis enabled separation of eight populations of C. spinosa. The AMOVA results revealed that the genetic variation among the populations (13%) was revealed to be significantly less pronounced than that observed within the some populations (87%). The STRUCTURE analysis and cluster analysis assumed from the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) highlighted three groups within the ISSR data. Our results suggested the presence of hybrid forms of C. spinosa in north-central Morocco. The raised levels of genetic variation revealed within the Moroccan Capparis population by ISSR markers indicate that an appropriate conservation program will allow capturing the majority of genetic diversity found within this species to help ensure its long-term breeding success.

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References

  • Allaith AAA (2016) Assessment of the antioxidant properties of the caper fruit (Capparis spinosa L.) from Bahrain. J Assoc Arab Univ Basic Appl Sci 19:1–7

    Google Scholar 

  • Andrade G, Esteban E, Velasco L, Lorite MJ, Bedmar EJ (1997) Isolation and identification of N2-fixing microorganisms from the rhizosphere of Capparis spinosa (L.). Plant Soil 197:19–23. https://doi.org/10.1023/A:1004211909641

    Article  CAS  Google Scholar 

  • Chakhchar A, Haworth M, El Modafar C, Lauteri M, Mattioni C, Wahbi S, Centritto M (2017) An assessment of genetic diversity and drought tolerance in argan Tree (Argania spinosa) populations: potential for the development of improved drought tolerance. Front Plant Sci 8:276

    Article  Google Scholar 

  • Chedraoui S, Abi-Rizk A, El-Beyrouthy M, Chalak L, Ouaini N, Rajjou L (2017) Capparis spinosa L. in a systematic review: a Xerophilous species of multi values and promising potentialities for agrosystems under the threat of global warming. Front Plant Sci 8:1845

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:3–15

    Google Scholar 

  • Eddouks M, Lemhadri A, Michel JB (2005) Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. J Ethnopharmacol 98:345–350

    Article  CAS  Google Scholar 

  • Eddouks M, Lemhadri A, Hebi M, Hidani AE, Zeggwagh NA, Bouhali BE, Hajji L, Burcelin R (2017) Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice. Avicenna J Phytomed 7:191–198

    PubMed  PubMed Central  Google Scholar 

  • El Amri N, Errachidi F, Bour A, Chabir R (2019b) Characterization of Moroccan raw and processed caper berries. Mater Today Proc 13:841–849

    Article  Google Scholar 

  • Amri NE, Errachidi F, Bour A, Bouhaddaoui S, Chabir R (2019a) Morphological and nutritional properties of Moroccan Capparis spinosa seeds. Sci World J. https://doi.org/10.1155/2019/8594820

    Article  Google Scholar 

  • Fici S (2001) Intraspecific variation and evolutionary trends in Capparis spinosa L. (Capparaceae). Plant Syst Evol 228:123–141

    Article  Google Scholar 

  • Fici S (2014) A taxonomic revision of the Capparis spinosa group (Capparaceae) from the Mediterranean to Central Asia. Phytotaxa 174:1–24

    Article  Google Scholar 

  • Fici S (2015) A taxonomic revision of the Capparis spinosa group (Capparaceae) from eastern Africa to Oceania. Phytotaxa 203:24–36. https://doi.org/10.11646/phytotaxa.203.1.2

    Article  Google Scholar 

  • Fu XP, Wu T, Abdurahim M, Su Z, Hou LX, Aisa HA, Wu H (2008) New spermidine alkaloids from Capparis spinosa roots. Phytochem Lett 1:59–62. https://doi.org/10.1016/j.phytol.2008.01.001

    Article  CAS  Google Scholar 

  • Gan L, Zhang C, Yin Y, Lin Z, Huang Y, Xiang JY, Fu C, Li M (2013) Anatomical adaptations of the xerophilous medicinal plant, Capparis spinosa, to drought conditions. Hortic Environ Biotechnol 54:156–161. https://doi.org/10.1007/s13580-013-0162-3

    Article  Google Scholar 

  • Gristina AS, Fici S, Siragusa M, Fontana I, Garfi G, Carimi F (2014) Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex. Flora 209:733–741. https://doi.org/10.1016/j.flora.2014.09.002

    Article  Google Scholar 

  • Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504

    Article  CAS  Google Scholar 

  • Higton RN, Akeroryd JR (1991) Variation in Capparis spinosa L. in Europe. Newton, flora Europea: notulea systematicae and floram europeam tantes. Series 2,4. Bot J Linn Soc 106:104–112

    Google Scholar 

  • Inocencio C, Alcaraz F, Calderon F, Obon C, Rivera D (2002) The use of floral characters in Capparis sect. Capparis’ to determine the botanical and geographical origin of capers. Eur Food Res Technol 214:335–339. https://doi.org/10.1007/s00217-001-0465-y

    Article  CAS  Google Scholar 

  • Inocencio C, Rivera D, Obón C, Alcaraz F, Barreña J (2006) A systematic revision of Capparis section Capparis (Capparaceae). Ann Missouri Bot Gard 93:122–149

    Article  Google Scholar 

  • Kala PC, Mathur BV (2002) Patterns of plant species distribution in the Trans-Himalayan region of Ladakh, India. J Veg Sci 13:751–754. https://doi.org/10.1111/j.1654-1103.2002.tb02104.x

    Article  Google Scholar 

  • Keylock CJ (2005) Simpson diversity and the Shannon-Wiener index as special cases of a generalized entropy. Oikos 109:203–207

    Article  Google Scholar 

  • Khanfar MA, Sabri SS, Zarga MH, Zeller KP (2003) The chemical constituents of Capparis spinosa of jordanian origin. Nat Prod Res 17:9–14. https://doi.org/10.1080/10575630290034302

    Article  CAS  PubMed  Google Scholar 

  • Kulisic-Bilusic T, Balzevic I, Dejanovic B, Milos M, Pifat G (2010) Evaluation of the antioxidant activity of essential oils from caper (Capparis spinosa) and sea fennel (Crithmum maritimum) by different methods. J Food Biochem 34:286–302. https://doi.org/10.1111/j.1745-4514.2009.00330.x

    Article  Google Scholar 

  • Lam SK, Ng TB (2009) A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from caper (Capparis spinosa) seeds. J Phytomed 16:444–450. https://doi.org/10.1016/j.phymed.2008.09.006

    Article  Google Scholar 

  • Mousavi SH, Hosseini A, Bakhtiari E, Rakhshandeh H (2015) Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in cardiomyoblast cells. Avicenna J Phytomed 6:489–494

    Google Scholar 

  • Muhaidat R, Al-Qudah AM, Al-Shayeb A, Jacob HJ, Al-Jaber H, Hussein E et al (2013) Chemical profile and antibacterial activity of crude fractions and essential oils of Capparis ovata Desf. and Capparis spinosa L. (Capparaceae). Int J Integr Biol 14:39–47

    Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    CAS  PubMed  PubMed Central  Google Scholar 

  • Nosrati H, Hosseinpour Feizi MA, Mazinani M, Haghighi AR (2012) Effect of population size on genetic variation levels in Capparis spinosa (Capparaceae) detected by RAPDs. Eur Asian J BioSci 6:70–75

    Article  Google Scholar 

  • Ozcan M, Hacıseferogullari H, Demir F (2004) Some physico-mechanic and chemical properties of capers (Capparis ovata Desf. var. canescens (Coss.) Heywood) flower buds. J Food Eng 65:151–155

    Article  Google Scholar 

  • Rhimi A, Mnasri S, Ben Ayed R, Bel Hajj Ali I, Hjaoujia S, Boussaid M (2019) Genetic relationships among subspecies of Capparis spinosa L. from Tunisia by using ISSR markers. Mol Biol Rep 46:2209–2219. https://doi.org/10.1007/s11033-019-04676-z

    Article  CAS  PubMed  Google Scholar 

  • Rhizopoulou S, Ioannidi E, Alexandredes N, Argiropoulos A (2006) A study on functional and structural traits of the nocturnal flowers of Capparis spinosa L. J Arid Environ 66:635–647

    Article  Google Scholar 

  • Rivera D, Inocencio C, Obon C, Alcaraz F (2003) Review of food and medicinal uses of Capparis L. subgenus Capparis (Capparidaceae). Econ Bot 57:515–534

    Article  Google Scholar 

  • Rodrigo M, Lazaro MJ, Alvarruiz A, Giner V (1992) Composition of capers (Capparis spinosa): influence of cultivar, size and harvest date. J Food Sci 57:1152–1154

    Article  CAS  Google Scholar 

  • Saadaoui E, Guetat A, Tlili N, El Gazzah M, Khaldi A (2011) Subspecific variability of Tunisian wild populations of Capparis spinosa L. J Med Plants Res 5:4339–4348

    Google Scholar 

  • Saifi N, Ibijbijen J, Echchghadda G (2011) Genetic diversity of Caper plant (Capparis sp.) from Morocco. J Food Agric Environ 9:299–304

    CAS  Google Scholar 

  • Sharaf M, El-Ansari MA, Saleh NAM (2000) Quercetin triglycoside from Capparis spinosa. Fitoterapia 71:46–49

    Article  CAS  Google Scholar 

  • Sher H, Alyemeni M (2010) Ethnobotanical and pharmaceutical evaluation of Capparis spinosa L, validity of local folk and Unani system of medicine. J Med Plants Res 4:1751–1756

    Google Scholar 

  • Sozzi GO (2001) Caperbush: botany and horticulture. Hortic Rev 27:125–188

    Google Scholar 

  • Zhang H, Ma ZF (2018) Phytochemical and pharmacological properties of Capparis spinosa as a medicinal plant. Nutrients 10:116. https://doi.org/10.3390/nu10020116

    Article  CAS  PubMed Central  Google Scholar 

  • Zuo W, Ma M, Ma Z, Gao R, Guo Y, Jiang W et al (2012) Study of photosynthetic physiological characteristics of desert plant Capparis spinosa L. J Shihezi Univ 3:006

    Google Scholar 

Download references

Acknowledgements

All authors acknowledge Dr. Jamal CHARAFI, from INRA-CRRA, Meknès, Maroc, for his help in laboratory analysis.

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Authors and Affiliations

  1. Laboratoire d’Agrobiotechnologie et Bioingénierie, Faculté des Sciences et Techniques Guéliz, Université Cadi Ayyad, Marrakech, Morocco

    Thouria Bourhim, Abdelaaziz Alaoui, Cherkaoui El Modafar & Driss Hsissou

  2. Laboratoire de Biotechnologie et Physiologie Végétales, Centre de Biotechnologie Végétale et Microbienne Biodiversité et Environnement, Faculté Des Sciences, Université Mohammed V de Rabat, 10000, Rabat, Morocco

    Abdelghani Chakhchar

  3. Institute of Botany, Developmental Biology of Plants, Justus-Liebig-University, Heinrich-Buff-Ring 38, 35392, Gießen, Germany

    Mouna Lamaoui

  4. African Sustainable Agriculture Research Institute (ASARI), Mohammed VI Polytechnic University (UM6P), Laâyoune, Morocco

    Youssef El Kharrassi

  5. Unité de Recherche Amélioration des Plantes et Conservation des Ressources Phytogénétiques, Centre Régional de la Recherche Agronomique, Meknès, Morocco

    Meriem Ibnou Ali El Alaoui

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  1. Thouria Bourhim
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  2. Abdelghani Chakhchar
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  3. Mouna Lamaoui
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  4. Youssef El Kharrassi
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  5. Abdelaaziz Alaoui
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  7. Meriem Ibnou Ali El Alaoui
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  8. Driss Hsissou
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Correspondence to Abdelghani Chakhchar.

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Communicated by M. Prasad.

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Bourhim, T., Chakhchar, A., Lamaoui, M. et al. Morphological characterization and assessment of genetic diversity of natural Moroccan populations of Capparis spinosa. Acta Physiol Plant 43, 37 (2021). https://doi.org/10.1007/s11738-021-03209-1

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  • DOI: https://doi.org/10.1007/s11738-021-03209-1

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