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Comparative analysis for understanding salinity tolerance mechanism in Indian Mustard (Brassica juncea L.)

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Abstract

This study reports a comparison of differential physiological and biochemical changes in two Indian Mustard (Brassica juncea L.) cultivars viz. CS-52 (salinity tolerant) and Ashirwad (salinity susceptible) after 15 days of gradual increase in NaCl concentration in the nutrient solution. The increase in the NaCl concentration in the nutrient solution was as follows: 25 mM for 2 days, 50 mM for 2 days, 75 mM for 2 days, 100 mM for 2 days, 125 mM for 2 days, and 150 mM for 5 days. After 15 days of salinity stress, we observed a sharp decline in dry matter content and leaf area in Ashirwad. These effects were, however, less pronounced in CS-52. Under high salinity conditions, CS-52 maintained a better physiological status as determined by higher relative water content, higher water use efficiency, and lower leaf temperature and electrolytic leakage ratio, compared to Ashirwad. CS-52 was also observed to be more efficient regarding gas-exchange parameters (stomatal conductance and transpiration) and photosynthetic capacity. Moreover, salt-induced changes in accumulation and distribution patterns, and the ratios of major macro- and microelements were recorded to be more favorable in CS-52 compared to Ashirwad. The study also revealed that salinity-induced relative changes in the concentrations and compositions of biomolecules such as lipids, proteins, and carbohydrates, and structural rearrangements in the side chains of proteins were less prominent in CS-52 indicating better preparedness and thus more adaptability of CS-52 towards salinity.

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Acknowledgements

We sincerely acknowledge Director, ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan (India), for providing financial support and the facilities to carry out this research work.

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Authors and Affiliations

  1. ICAR-Indian Institute of Agricultural Biotechnology, Ranchi, Jharkhand, 834 010, India

    Binay K. Singh, Avinash Pandey, Sudhir Kumar, Devendra K. Singh & Saurav Kumar

  2. ICAR-Indian Institute of Sugarcane Research, Lucknow, Uttar Pradesh, 226 002, India

    Surendra P. Singh

  3. ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    Kapila Shekhawat & Sanjay S. Rathore

  4. ICAR-National Bureau of Plant Genetic Resources, RS, Ranchi, Jharkhand, 834 010, India

    Shashi Bhushan Choudhry

  5. ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan, 321 303, India

    Dhiraj Singh

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  1. Binay K. Singh
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Correspondence to Binay K. Singh.

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Singh, B.K., Singh, S.P., Shekhawat, K. et al. Comparative analysis for understanding salinity tolerance mechanism in Indian Mustard (Brassica juncea L.). Acta Physiol Plant 41, 104 (2019). https://doi.org/10.1007/s11738-019-2894-x

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