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Exogenous hydrogen peroxide enhanced the thermotolerance of Festuca arundinacea and Lolium perenne by increasing the antioxidative capacity

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Abstract

Heat stress is one of the most detrimental environment stresses for plants. Hydrogen peroxide (H2O2) is produced quickly in response to various stresses and likely plays a positive role in transmitting stress signal in organisms. This investigation addressed whether an exogenous H2O2 application would affect the heat response of turfgrasses and induce acclimation. Tall fescue (Festuca arundinacea cv. Barlexas) and perennial ryegrass (Lolium perenne cv. Accent), two important cool-season turfgrasses and forages, were sprayed with 10 mM H2O2 before they were treated with heat stress (38/30 °C, day/night) and compared with plants maintained at control temperatures (26/15 °C, day/night). Prior to the initiation of heat stress, H2O2 pretreatment increased the activities of guaiacol peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR) and glutathione-dependent peroxidase (GPX) and the ascorbate and glutathione pool, and it decreased the GSH/GSSG ratio. During the heat stress process, pretreated plants from both grasses exhibited higher turfgrass quality and relative water content, and they experienced lower oxidative damage and H2O2 levels. Moreover, the activities of APX, GR, GPX and glutathione-S-transferase increased significantly in response to H2O2 pretreatment under heat stress. These results suggested that H2O2 most likely participated in the transduction of redox signaling and induced the antioxidative defense system, including various enzymatic and nonenzymatic H2O2 scavengers. The scavengers played important roles in improving the thermotolerance of tall fescue and perennial ryegrasses.

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Acknowledgments

This research was supported by the West Light Foundation of The Chinese Academy of Sciences (Y3C4011100), and the National High Technology Rzesearch and Development Program of China (863 Program) (2009AA10Z108, 2008AA10Z409).

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Authors and Affiliations

  1. Chengdu Institute of Biology, Chinese Academic of Science, Chengdu, 610041, People’s Republic of China

    Yan Wang & Xin-Rong Ma

  2. Nanjing Forest Police College, Nanjing, 210046, People’s Republic of China

    Jie Zhang

  3. School of Life Sciences, Nanjing University, Nanjing, 210093, People’s Republic of China

    Jian-Long Li

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  1. Yan Wang
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  2. Jie Zhang
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  4. Xin-Rong Ma
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Correspondence to Xin-Rong Ma.

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Communicated by A. Gniazdowska-Piekarska.

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Wang, Y., Zhang, J., Li, JL. et al. Exogenous hydrogen peroxide enhanced the thermotolerance of Festuca arundinacea and Lolium perenne by increasing the antioxidative capacity. Acta Physiol Plant 36, 2915–2924 (2014). https://doi.org/10.1007/s11738-014-1661-2

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  • DOI: https://doi.org/10.1007/s11738-014-1661-2

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