Study on the differences between moxibustion at different points in the effects on the anti-fatigue ability of rats undergoing one-time exhaustive swimming

Abstract

Objective

To observe the effects of moxibustion at Shenshu (BL 23), Zusanli (ST 36) and Shenque (CV 8) on the energy metabolism and endocrine metabolism indicators of rats undergoing one-time exhaustive swimming, and to explore the differences between moxibustion at different points in the effects on anti-exercise fatigue.

Methods

Forty-eight male SPF rats were randomly divided into a blank group, a model group, a non-meridian and non-acupoint group, a Shenshu (BL 23) group, a Zusanli (ST 36) group, and a Shenque (CV 8) group using random number table method, with eight rats in each group. Except for the blank group, rats in the other groups were subjected to replicating the one-time exhaustive model using the weight-bearing swimming experiment. Except for the model group, the other model rats received mild moxibustion immediately after swimming. Rats in the non-meridian and non-acupoint group received mild moxibustion at bilateral subcostal non-meridian and non-acupoint points, those in the Shenshu (BL 23) group received mild moxibustion at bilateral Shenshu (BL 23), those in the Zusanli (ST 36) group received mild moxibustion at bilateral Zusanli (ST 36), and those in the Shenque (CV 8) group received mild moxibustion at Shenque (CV 8) for 15 min. Four hours after the exhaustive swimming, femoral artery blood was collected to detect blood lactate (BLA), lactate dehydrogenase (LDH), creatine kinase (CK), creatinine (CRE), blood urea nitrogen (BUN), cortisol (C) and testosterone (T) levels, and calculate the T/C ratio.

Results

Compared with the blank group, rat’s serum levels of BLA, LDH, CK, BUN and C in the model group and the non-meridian and non-acupoint group were increased, and serum levels of CRE and T, and T/C ratios were decreased (P<0.01 or P<0.05); compared with the model group and the non-meridian and non-acupoint group, the serum levels of BLA, LDH, CK, BUN and C in the Shenshu (BL 23) group, Zusanli (ST 36) group and Shenque (CV 8) group were decreased, and the serum CRE and T levels, and the T/C ratios were increased (all P<0.01); compared with the Shenshu (BL 23) group, the serum CK level was decreased in the Shenque (CV 8) group (P<0.01), the serum levels of T and C were decreased in the Zusanli (ST 36) group and Shenque (CV 8) group (P<0.01 or P<0.05), and the T/C ratio was increased in the Shenque (CV 8) group (P<0.01); compared with the Zusanli (ST 36) group, the serum CK and BUN levels were decreased (P<0.01, P<0.05), and the T/C ratio was increased in the Shenque (CV 8) group (P<0.05).

Conclusion

Moxibustion at Shenshu (BL 23), Zusanli (ST 36) and Shenque (CV 8) shows different anti-fatigue effects by regulating the energy metabolism and endocrine metabolism in rats undergoing one-time exhaustive swimming. Moxibustion at Shenshu (BL 23) is better in promoting energy synthesis. Moxibustion at Shenque (CV 8) is more effective in regulating synthesis and decomposition of the skeletal muscle proteins.

摘要

目的

观察艾灸肾俞、足三里和神阙对一次性力竭大鼠能量代谢和内分泌代谢指标的影响, 探讨艾灸不 同腧穴抗运动疲劳作用的差异性.

方法

将48只雄性SPF级大鼠采用随机数字表法随机分为空白组、模型组、非 经非穴组、肾俞组、足三里组和神阙组, 每组8只。除空白组外, 其余各组大鼠采用负重游泳实验复制一次性力 竭模型。除模型组外, 其余造模大鼠游泳结束后即刻采用温和灸疗法进行干预。非经非穴组温和灸双侧肋下非经 非穴点, 肾俞组温和灸双侧肾俞, 足三里组温和灸双侧足三里, 神阙组温和灸神阙。各组均治疗15 min。力竭游 泳后4 h进行股动脉采血, 检测血清血乳酸(BLA)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酐(CRE)、血尿素氮(BUN)、 皮质醇(C)及睾酮(T)含量, 并计算T/C值.

结果

与空白组相比, 模型组和非经非穴组大鼠血清BLA、LDH、CK、BUN 和C含量升高, 血清CRE和T含量及T/C值降低(P<0.01或P<0.05); 与模型组和非经非穴组相比, 肾俞组、足三里组 和神阙组血清BLA、LDH、CK、BUN和C含量降低, 血清CRE和T含量及T/C值升高(均P<0.01); 与肾俞组相比, 神阙 组血清CK降低(P<0.01), 足三里组和神阙组血清T和C降低(P<0.01或P<0.05), 神阙组血清T/C值升高(P<0.01); 与足三里组相比, 神阙组血清CK降低(P<0.01), 血清BUN含量下降(P<0.05), T/C值升高(P<0.05).

结论

艾灸肾俞、 足三里和神阙均可通过调节一次性力竭大鼠的能量代谢和内分泌代谢发挥抗疲劳作用, 但存在一定差异性, 艾 灸肾俞在促进能量合成方面效果较好, 艾灸神阙在调节骨骼肌蛋白质合成与分解方面效果较好.