Effect of moxibustion at Shenque (CV 8) on free radical metabolism in rat’s hippocampus after different degrees of exhaustive exercise

Abstract

Objective

To investigate the effect of moxibustion at Shenque (CV 8) on free radical metabolism in rat’s hippocampus after different degrees of exhaustive exercise.

Methods

A total of 72 male Sprague-Dawley (SD) rats were randomly divided into a normal group (n=8), a model group (n=32) and a moxibustion group (n=32). According to the times of modeling or treatment, the model group was further randomly divided into different subgroups of a 1-time model subgroup, a 4-time model subgroup, a 7-time model subgroup and a 10-time model subgroup (n=8); the moxibustion group was also further randomly divided into different subgroups of a 1-time moxibustion subgroup, a 4-time moxibustion subgroup, a 7-time moxibustion subgroup and a 10-time moxibustion subgroup (n=8). Rats in both model and moxibustion subgroups were subjected to establishing the swimming exhaustive model. Rats in each moxibustion subgroup received mild moxibustion at Shenque (CV 8) for 15 min immediately after modeling, once every other day. The concentration of malic dialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in rat’s hippocampus in each group were detected 24 h after the exhaustive exercise.

Results

Compared with the 1-time model subgroup, the exhaustive swimming time of rats was significantly prolonged in the 4-time model subgroup (P<0.01), while it was significantly shortened in the 7-time and 10-time model subgroups (both P<0.01). Compared with the matched model subgroup, the exhaustive swimming time of rats in the 7-time and 10-time moxibustion subgroups was significantly prolonged (both P<0.01). Compared with the normal group, the MDA concentration was increased significantly (P<0.01), and the activities stress response of SOD and T-AOC were increased in the 1-time model subgroup (both P<0.05); the MDA concentration was increased (all P<0.01), and the activities of SOD, GSH-Px and T-AOC were decreased differently (P<0.05 or P<0.01) in the 4-time, 7-time and 10-time model subgroups. Compared with the matched model subgroup, the concentration of MDA was significantly reduced (P<0.05 or P<0.01), and the activities of SOD, GSH-Px and T-AOC were significantly increased in the 4-time, 7-time and 10-time moxibustion subgroups (all P<0.01).

Conclusion

Moxibustion at Shenque (CV 8) can improve the fatigue status of the body after long-term exhaustive exercise by regulating free radical metabolism in rat’s hippocampus. To some extent, this provides an experimental basis for moxibustion at Shenque (CV 8) against exercise-induced fatigue.

摘要

目的

探讨艾灸神阙穴对不同程度力竭运动大鼠海马区自由基代谢的影响。

方法

将72只雄性Sprague-Dawley (SD)大鼠随机分为正常组(n=8)、模型组(n=32)和艾灸组(n=32), 根据造模或治疗的时间, 将模型组与艾灸组再分别随机分为模型1次亚组、模型4次亚组、模型7次亚组、模型10次亚组和艾灸1次亚组、艾灸4次亚组、艾灸7次亚组和艾灸10次亚组, 每亚组8只。模型亚组及艾灸亚组大鼠复制游泳力竭模型。艾灸各亚组大鼠造模后即刻温和灸神阙穴15 min, 隔日1次。各组大鼠完成相应力竭运动24 h后, 检测海马组织丙二醛(MDA)含量, 及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和总抗氧化能力(T-AOC)活性。

结果

与模型1次亚组比较, 模型4次亚组大鼠游泳力竭时间明显延长(P<0.01), 模型7次亚组和模型10次亚组均明显缩短(均P<0.01)。与相应模型亚组比较, 艾灸7次亚组和艾灸10次亚组大鼠游泳时间均明显延长(均P<0.01)。与正常组比较, 模型1次亚组MDA含量明显增加(P<0.01), SOD和T-AOC活性应激性增强(均P<0.05); 模型4次亚组、模型7次亚组和模型10次亚组中MDA含量增加(均P<0.01), SOD、GSH-Px和T-AOC活性出现不同程度下降(P<0.05或P<0.01)。与相应模型亚组比较, 艾灸4次亚组、艾灸7次亚组和艾灸10次亚组MDA含量均明显降低(P<0.05或P<0.01), SOD、GSH-Px和T-AOC活性均明显增强(P<0.01)。

结论

艾灸神阙穴可通过调节长期力竭运动大鼠海马区自由基代谢, 改善机体疲劳状态, 一定程度上为艾灸神阙穴抗运动性疲劳提供了实验依据。