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Effect of electroacupuncture on expressions of VEGF and CD31 in MCAO model rats

电针对MCAO 模型大鼠VEGF 和CD31 表达的影响

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Abstract

Objective

To investigate the effect of electroacupuncture (EA) at Ganshu (BL 18) and Shenshu (BL 23) on vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecule-1 (PECAM-1)/CD31 around the cerebral infarction focus in middle cerebral artery occlusion (MCAO) rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture.

Methods

A total of 180 healthy male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu (BL 18) and Shenshu (BL 23); rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected.

Results

Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant (P<0.05, P<0.01). The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day (P<0.05, P<0.01). Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant (all P<0.05).

Conclusion

EA at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.

摘要

目的

探讨电针肝俞和肾俞对大脑中动脉梗塞(MCAO)模型大鼠脑梗死灶周围血管新生相关因子血管内皮 生长因子(VEGF)、血小板-内皮细胞粘附分子(PECAM-1)/CD31的影响及可能机制, 为针刺治疗脑缺血中风提供新方 案。

方法

将180只健康雄性Sprague-Dawley (SD)大鼠随机分为假手术组、模型组、穴位组和非穴组, 每组45只。 除假手术组外, 其余各组大鼠均采用改良线栓法制备MCAO模型; 穴位组予电针肝俞和肾俞治疗, 非穴组予电针 非穴点治疗, 其余两组大鼠只捆绑, 不治疗。在MCAO术后针灸刺激的第3 d、14 d及21 d三个时间点各组随机抽10 只大鼠测试大鼠神经缺损症状; 同时检测CD31和VEGF的表达量。

结果

与模型组和非穴组比较, 穴位组各时相的 神经功能评分降低, 组间差异具有统计学意义(P<0.05, P<0.01)。各组大鼠VEGF和CD31的表达在第3 d时最低, 于 14 d达高峰, 第21 d仍维持在较高水平, 各组第14 d与第21 d比较均有统计学意义(P<0.05, P<0.01)。与模型组及 非穴组比较, 穴位组各时相VEGF和CD31的表达升高, 组间差异具有统计学意义(均P<0.05)。

结论

电针肝俞和肾 俞能明显改善MCAO模型大鼠神经功能评分, 对脑缺血有保护作用, 保护机制可能与电针上调MCAO模型大鼠梗 死灶周围CD31和VEGF表达, 诱导血管新生有关。

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References

  1. Yates HL, Mccullough S, Harrison C, Harrison, Gill AB. Hypoxic ischaemic encephalopathy: accuracy of the reported incidence. Arch Dis Child Fetal Neonatal Ed, 2012, 97(1): F77–F78.

    Article  CAS  PubMed  Google Scholar 

  2. Fan YW, Dai DW, Wu SS, Gao J, Mu WW, Zhang LM. Research progress on secondary prevention of ischemic stroke. Xiandai Shengwu Yixue Jinzhan, 2015, 15(12): 2382–2385.

    CAS  Google Scholar 

  3. Yu CQ, Zhang M, Xue M, Gong K. The distribution pattern of cerebral artery atherosclerotic and the profiles of related risk factors among patients with ischemic cerebrovascular disease. Shenjingbingxue Yu Shengjingkangfuxue Zazhi, 2010, 7(3): 142–143.

    Google Scholar 

  4. Yang Y, Sun H. Research progress of acupuncture for cerebral ischemia reperfusion injury in recent 10 years. Zhongguo Zhen Jiu, 2015, 35(7): 749–752.

    PubMed  Google Scholar 

  5. Development Group of Guidelines for Diagnosis and Treatment of Acute Ischemic Stroke, Cerebrovascular Disease Group, Neurology Branch of Chinese Medical Association. Guidelines for diagnosis and treatment of acute ischemic strokein China 2010. Zhonghua Shenjingke Zazhi, 2010, 43(2): 146–152.

    Google Scholar 

  6. Li TL. Comparison on Therapeutic Effects by Electroacupuncturing Different Points on Rats with Acute Cerebral Ischemia and Study of the Related Mechanism. Changsha: Doctor Thesis of Hunan University of Chinese Medicine, 2005.

    Google Scholar 

  7. Xing SH. The Effects of EphB2 on the Neurogenesis and Angiogenesis after Cerebral Cortex Infarction in Hypertensive Rats. Guangzhou: Doctor Thesis of Zhongshan University, 2009.

    Google Scholar 

  8. Solowiej A, Biswas P, Graesser D, Madri JA. Lack of platelet endothelial cell adhesionmolecule-1 attenuates foreign body inflammation because of decreased angiogenesis. Am J Patho, 2003, 162(3): 953–962.

    Article  CAS  Google Scholar 

  9. Li ZR. Experimental Acupuncture Science. Beijing: China Press of Traditional Chinese Medicine, 2003: 327–329.

    Google Scholar 

  10. Zhang XC, Zhao H, Ma JH, Liu N. Experimental study on the model of focal cerebral ischemia in rats with suture-occluded method. Yixue Yanjiu Zazhi, 2013, 42(6): 55–58.

    CAS  Google Scholar 

  11. Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989, 20(1): 84–91.

    Article  CAS  PubMed  Google Scholar 

  12. Li HL, Xiang J, Ouyang LZ, Xue ZH, Long KS, Li TL. Effect of electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat. J Acupunct Tuina Sci, 2017, 15(1): 14–21.

    Article  Google Scholar 

  13. Zhang H, Wang ZZ, Zhang YC, Yang LJ, Deng SF, Ai K, Zhang ZP. Effect of electroacupuncture of Xiaohai (SI 8) and Xiajuxu (ST 39) on serum TNF-α and duodenal high mobility group protein B1 levels in duodenal ulcer rats. Zhen Ci Yan Jiu, 2015, 40(1): 35–39.

    CAS  PubMed  Google Scholar 

  14. Liu ZB, Niu XM. Nenroantomical basis of the location of Back-Shu points. Zhongguo Zhongyi Jichu Yixue Zazhi, 2013, 19(1): 83–85.

    CAS  Google Scholar 

  15. Jin X, Wang RH, Wang H, Long CL, Wang H. Brain protection against ischemic stroke using choline as a new molecular bypass treatment. Acta Pharmacol Sin, 2015, 36(12): 1416–1425.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Chávez JC, Agani F, Pichiule P, Lammanna JC. Expression of hypoxia-inducible factor-1alpha in the brain of rats during chronic hypoxia. J Appl Physiol (1985), 2000, 89(5): 1937–1942.

    Google Scholar 

  17. Marti HJ, Bernaudin M, Bellail A, Schoch H, Euler M, Petit E, Risau W. Hypoxia-induced vascular endothelial growth factor expression precedes neovascularization after cerebral ischemia. Am J Pathol, 2000, 156(3): 965–976.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Xu H, Sun H. Acupuncture therapy for ischemic stroke in model of rats (review). Zhongguo Kangfu Lilun Yu Shijian, 2009, 15(3): 214–215.

    Google Scholar 

  19. Chen CY, Li Q, Wang Y. Research progress of endothelial progenitor cells derived extracellular vesicles. Zhongguo Xiufu Chongjian Waike Zazhi, 2015, 29(9): 1155–1158.

    Google Scholar 

  20. Corre P, Merceron C, Vignes C, Sourice S, Masson M, Durand N, Espitalier F, Pilet P, Cordonnier T, Mercier J, Remy S, Anegon I, Weiss P, Guicheux J. Determining a clinically relevant strategy for bone tissue engineering: an ‘all-in-one’ study in nude mice. PLoS One, 2013, 8(12): e81599.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Zuo C, Chen BG, Ding L, Zhang XM. Influence of electroacupuncture on brain tissues CD31 of rats with focal cerebral ischemia-reperfusion (FCIR). Shaanxi Zhongyi Xueyuan Xuebao, 2012, 35(1): 54–56.

    Google Scholar 

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Acknowledgments

This work was supported by Open Fund for Colleges and Universities Innovation Platform of Hunan Province (湖南 省高校创新平台开放基金, No.14K070); Key Project of Hunan Province Administration of Traditional Chinese Medicine (湖南省中医药管局重点项目, No. 201310).

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Authors and Affiliations

  1. School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208, China

    Guo Chen  (陈果), Juan Xiang  (向娟), Li-zhi Ouyang  (欧阳里知), Jin Song  (宋瑾), Zhi-hui Xue  (薛智慧), Hong-liang Li  (李洪亮) & Tie-lang Li  (李铁浪)

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  1. Guo Chen  (陈果)
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  2. Juan Xiang  (向娟)
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  3. Li-zhi Ouyang  (欧阳里知)
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  6. Hong-liang Li  (李洪亮)
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Correspondence to Tie-lang Li  (李铁浪).

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Chen, G., Xiang, J., Ouyang, Lz. et al. Effect of electroacupuncture on expressions of VEGF and CD31 in MCAO model rats. J. Acupunct. Tuina. Sci. 15, 311–316 (2017). https://doi.org/10.1007/s11726-017-1020-0

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  • DOI: https://doi.org/10.1007/s11726-017-1020-0

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