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Effect of electroacupuncture at Neiguan (PC 6) and Baihui (GV 20) on CHOP and caspase-12 gene expressions in rats after ischemia-reperfusion injury

电针内关、百会对缺血再灌注损伤大鼠CHOP 和caraspase-12 基因表达的影响

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Abstract

Objective

To investigate the effects of electroacupuncture (EA) at Neiguan (PC 6) and Baihui (GV 20) by observing the changes of CCAAT/enhancer-binding protein(C/EBP) homologous protein (CHOP) and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury (IRI), and explore whether the apoptosis pathway of endoplasmic reticulum stress (ERS) is involved in the protective mechanisms of EA.

Methods

Sixty rats were randomly assigned to five groups (12 in each group): a normal control group (group A), a sham-operation group (group B), an operation group (group C), an Edaravone group (group D) and an EA group (group E). The cerebral IRI rat model was induced by middle cerebral artery occlusion (MCAO) using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride (TTC) staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction (RT-PCR) was used to determine the mRNA expressions of CHOP and caspase-12.

Results

Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances (P<0.05 or P<0.01); compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly (P<0.05 or P<0.01); there were no significant differences between group D and group E in comparing the above items (P>0.05).

Conclusion

EA at Neiguan (PC 6) and Baihui (GV 20) can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan (PC 6) and Baihui (GV 20) is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.

摘要

目的

通过观察CHOP 和Caspase-12 基因表达在脑缺血再灌注大鼠体内的变化探讨电针内关、百会的效 应以及电针保护性机制是否与内质网应激介导的凋亡通路有关。

方法

将60 只大鼠随机分为5 组, 每组12 只, 即正常对照组(A)、假手术组(B)、模型组(C)、依达拉奉组(D)、电针干预组(E)。采用线栓法结扎大脑中动脉制备大 鼠局灶性脑缺血再灌注模型, 2,3,5-氯化三苯基四氮唑(TTC)染色测定脑梗死体积, RT-PCR 法测定缺血侧顶叶大脑皮 层CHOP、caspase-12 mRNA 表达变化。

结果

与正常组(A)和假手术组(B)比较, 模型组(C)、依达拉奉组(D)和电针 组(E)脑梗死体积百分比、CHOP、caspase-12 mRNA 表达均显著增高(P<0.01 或P<0.05); 与模型组(C)比较,依达拉 奉组(D)和电针组(E)脑梗死体积、CHOP、caspase-12 mRNA 表达均显著降低(P<0.01 或P<0.05);与依达拉奉组(D) 比较,电针组(E)脑梗死体积、CHOP、caspase-12 mRNA 表达无显著差异(P>0.05)。

结论

电针内关、百会可以有效 降低大鼠脑梗死体积,其脑保护潜在机制可能与下调凋亡因子CHOP、caspase-12 mRNA 表达, 从而抑制神经细胞 凋亡相关。

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Acknowledgments

This work was supported by National Natural Science Foundation of China (国家自然科学基金, No.81202771)

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Authors and Affiliations

  1. School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208, China

    Wen-hui Li  (李文慧), Xing Tan  (谭杏) & Yang Song  (宋洋)

  2. Postgraduate School of Hunan University of Chinese Medicine, Changsha, 410208, China

    Jie Yu  (郁洁) & Ya-ping Lin  (林亚平)

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  1. Wen-hui Li  (李文慧)
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  2. Jie Yu  (郁洁)
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  3. Ya-ping Lin  (林亚平)
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Correspondence to Ya-ping Lin  (林亚平).

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Li, Wh., Yu, J., Lin, Yp. et al. Effect of electroacupuncture at Neiguan (PC 6) and Baihui (GV 20) on CHOP and caspase-12 gene expressions in rats after ischemia-reperfusion injury. J. Acupunct. Tuina. Sci. 15, 8–13 (2017). https://doi.org/10.1007/s11726-017-0967-1

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  • DOI: https://doi.org/10.1007/s11726-017-0967-1

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