Abstract
The metabolic profiles of seed extracts from two cultivars of avocado (Hass var. and Criollo var., thereafter Hass and Criollo) were obtained using FTIR and 1H NMR spectroscopies. The extracts can be regarded as a rich source of bioactive compounds with significant antioxidant and antimicrobial properties. The extraction yields was 25.5% for Hass and 17.5% for Criollo. Total phenolic content and antioxidant activity were higher for Hass (287.41 ± 1.36 mg GAE/g dry extract and 1329.48 ± 6.89 μmol Fe + 2/g dry seed) as compared to Criollo (207.01 ± 1.38 mg GAE/g dry extract and 1276.18 ± 6.4 μmol Fe + 2/g dry seed). Also, the composition of the extracts was determined by using UPLC-ESI-QTOF-MS/MS. Eleven compounds were identified in both extracts, which included mainly phenolic acids, epicatechin derivatives and flavonoids. These compounds were correlated to the profiles obtained with FTIR and 1H NMR. A higher inhibition growth of S. aureus was observed for Hass extracts. The results obtained here suggest that avocado seeds from Hass variant are a substantial source of bioactive compounds, which may have significant potential in the food industry.
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References
Acosta-Estrada BA, Gutiérrez-Uribe JA, Serna-Saldı́var SO (2014) Bound phenolics in foods, a review. Food Chem 152:46–55. https://doi.org/10.1016/j.foodchem.2013.11.093
Bahru TB, Tadele ZH, Ajebe EG (2019) A review on avocado seed: functionality, composition, antioxidant and antimicrobial properties. Chem Sci Int J 27(2):1–10. https://doi.org/10.9734/CSJI/2019/v27i230112
Baldassarre F, Vergaro V, De Castro F et al (2022) Enhanced bioactivity of pomegranate peel extract following controlled release from caco 3 nanocrystals. Bioinorg Chem Appl. https://doi.org/10.1155/2022/6341298
Barbera M (2020) Reuse of food waste and wastewater as a source of polyphenolic compounds to use as food additives. J AOAC Int 103(4):906–914. https://doi.org/10.1093/jaocint/qsz025
Bautista-Hernández I, Aranda-Ledesma NE, Rojas R et al (2021) Antioxidant activity of polyphenolic compounds obtained from euphorbia antisyphilitica by-products. Heliyon. https://doi.org/10.1016/j.heliyon.2021.e06734
Benzie IF, Devaki M (2018) The ferric reducing/antioxidant power (FRAP) assay for non-enzymatic antioxidant capacity: concepts, procedures, limitations and applications. Wiley, Chap 5, pp 77–106. https://doi.org/10.1002/9781119135388.ch5
Bettuzzi S, Gabba L, Cataldo S (2021) Efficacy of a polyphenolic, standardized green tea extract for the treatment of covid-19 syndrome: a proof-of-principle study. COVID 1(1):2–12. https://doi.org/10.3390/covid1010002
Cavalieri S, Harbeck R, McCarter Y et al (2005) Prueba de difusi on por disco. Manual de Pruebas de Susceptibilidad Antimicrobiana, pp 39–52
FAOSTAT (2024) Food and Agriculture Organization-Statistical dataset. https://www.fao.org/faostat/en/#data/QCL. Accessed 28 Jan 2024
Gigliobianco MR, Cortese M, Nannini S et al (2022) Chemical, antioxidant, and antimicrobial properties of the peel and male flower by-products of four varieties of punica granatum l. Cultivated in the marche region for their use in cosmetic products. Antioxidants. https://doi.org/10.3390/antiox11040768
Gnaim R, Unis R, Gnayem N et al (2023) Avocado seed waste bioconversion into poly(3-hydroxybutyrate) by using cobetia amphilecti and ethyl levulinate as a green extractant. Int J Biol Macromol 239:124371. https://doi.org/10.1016/j.ijbiomac.2023.124371
Gutiérrez-del Río I, López-Ibáñez S, Magadán-Corpas P et al (2021) Terpenoids and polyphenols as natural antioxidant agents in food preservation. Antioxidants 10(8):234. https://doi.org/10.3390/antiox10081264
Huang L, Yang KP, Zhao Q et al (2022) Corrosion resistance and antibacterial activity of procyanidin b2 as a novel environment-friendly inhibitor for q235 steel in 1 m hcl solution. Bioelectrochemistry 143:107969. https://doi.org/10.1016/j.bioelechem.2021.107969
Jimenez P, Garcia P, Quitral V et al (2021) Pulp, leaf, peel and seed of avocado fruit: a review of bioactive compounds and healthy benefits. Food Rev Int 37(6):619–655. https://doi.org/10.1080/87559129.2020.1717520
Karar ME, Kuhnert N (2015) Uplc-esi-q-tof-ms/ms characterization of phenolics from crataegus monogyna and crataegus laevigata (hawthorn) leaves, fruits and their herbal derived drops (crataegutt tropfen). J Chem Biol Ther 1(102):2572–3406. https://doi.org/10.4172/2572-0406.1000102
Kaur Kala H, Mehta R, Tandey R et al (2016) Ten years of research on phenolics (2005–2015): a status report. Pac Sci Rev A Nat Sci Eng 18(1):1–4. https://doi.org/10.1016/j.psra.2016.07.002
Kosińska A, Karamać M, Estrella I et al (2012) Phenolic compound profiles and antioxidant capacity of persea americana mill. peels and seeds of two varieties. J Agric Food Chem 60(18):4613–4619. https://doi.org/10.1021/jf300090p
Liu Y, Benohoud M, Galani Yamdeu JH et al (2021) Green extraction of polyphenols from citrus peel by-products and their antifungal activity against aspergillus flavus. Food Chem X 12:100144. https://doi.org/10.1016/j.fochx.2021.100144
Lucarini M, Durazzo A, Kiefer J et al (2020) Grape seeds: Chromatographic profile of fatty acids and phenolic compounds and qualitative analysis by ftir-atr spectroscopy. Foods. https://doi.org/10.3390/foods9010010
Magar RT, Sohng JK (2020) A review on structure, modifications and structure-activity relation of quercetin and its derivatives. J Microbiol Biotechnol 30(1):11–20. https://doi.org/10.4014/jmb.1907.07003
Melgar B, Dias MI, Ciric A et al (2018) Bioactive characterization of persea americana mill. by-products: a rich source of inherent antioxidants. Ind Crops Prod 111:212–218. https://doi.org/10.1016/j.indcrop.2017.10.024
Pérez-Saucedo M, Jiménez-Ruiz E, Rodrı́guez-Carpena J, et al (2021) Properties of the avocado oil extracted using centrifugation and ultrasound-assisted methods. Food Sci Biotechnol 30:1051–1061. https://doi.org/10.1007/s10068-021-00940-w
Razmavar S, Abdulla MA, Ismail SB et al (2014) Antibacterial activity of leaf extracts of baeckea frutescens against methicillin-resistant staphylococcus aureus. Biomed Res Int. https://doi.org/10.1155/2014/521287
Rosero JC, Cruz S, Osorio C et al (2019) Analysis of phenolic composition of byproducts (seeds and peels) of avocado (persea americana mill.) cultivated in colombia. Molecules 24(17):234. https://doi.org/10.3390/molecules24173209
Ruiz-Aquino F, Feria-Reyes R, Rutiaga-Quiñones JG et al (2023) Characterization of tannin extracts derived from the bark of four tree species by hplc and ftir. For Sci Technol 19(1):38–46. https://doi.org/10.1080/21580103.2023.2166593
Salazar-López NJ, Domı́nguez-Avila JA, Yahia EM et al (2020) Avocado fruit and by-products as potential sources of bioactive compounds. Food Res Int. https://doi.org/10.1016/j.foodres.2020.109774
Sánchez H, Ponce W, Brito B, et al (2021) Biofilms production from avocado waste. Ingenierı́a y Universidad 25:1–16. https://doi.org/10.11144/Javeriana.iued25.bpaw
Saridewi N, Adinda AR, Nurbayti S (2022) Characterization and antibacterial activity test of green synthetic zno nanoparticles using avocado (persea americana) seed extract. Jurnal Kimia Sains Dan Aplikasi 25(3):116–122. https://doi.org/10.14710/jksa.25.3.116-122
Silva V, Falco V, Dias MI et al (2020) Evaluation of the phenolic profile of castanea sativa mill. by-products and their antioxidant and antimicrobial activity against multiresistant bacteria. Antioxidants 9(1):234. https://doi.org/10.3390/antiox9010087
Soldera-Silva A, Seyfried M, Campestrini LH et al (2018) Assessment of anthelmintic activity and bio-guided chemical analysis of persea americana seed extracts. Vet Parasitol 251:34–43. https://doi.org/10.1016/j.vetpar.2017.12.019
Soong YY, Barlow PJ (2004) Antioxidant activity and phenolic content of selected fruit seeds. Food Chem 88(3):411–417. https://doi.org/10.1016/j.foodchem.2004.02.003
Swarup Roy AK, Ezati P, Rhim JW (2023) New opportunities and advances in quercetin-added functional packaging films for sustainable packaging applications: a mini-review. Crit Rev Food Sci Nutr. https://doi.org/10.1080/10408398.2023.2200553
Tremocoldi MA, Rosalen PL, Franchin M et al (2018) Exploration of avocado byproducts as natural sources of bioactive compounds. PLoS ONE 13(2):1–12. https://doi.org/10.1371/journal.pone.0192577
Ullah H, Hussain Y, Santarcangelo C et al (2022) Natural polyphenols for the preservation of meat and dairy products. Molecules. https://doi.org/10.3390/molecules27061906
Velderrain-Rodriguez GR, Quero J, Osada J et al (2021) Phenolic-rich extracts from avocado fruit residues as functional food ingredients with antioxidant and antiproliferative properties. Biomolecules. https://doi.org/10.3390/biom11070977
Vodnar DC, Călinoiu LF, Dulf FV et al (2017) Identification of the bioactive compounds and antioxidant, antimutagenic and antimicrobial activities of thermally processed agro-industrial waste. Food Chem 231:131–140. https://doi.org/10.1016/j.foodchem.2017.03.131
Wang W, Bostic TR, Gu L (2010) Antioxidant capacities, procyanidins and pigments in avocados of different strains and cultivars. Food Chem 122(4):1193–1198. https://doi.org/10.1016/j.foodchem.2010.03.114
Xu B, Chang S (2007a) A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 72(2):S159–S166. https://doi.org/10.1111/j.1750-3841.2006.00260.x
Xu BJ, Chang SKC (2007b) A Comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 72:S159–S166. https://doi.org/10.1111/j.1750-3841.2006.00260.x
Yang Q, Liang Q, Balakrishnan B et al (2020) Role of dietary nutrients in the modulation of gut microbiota: a narrative review. Nutrients. https://doi.org/10.3390/nu12020381
Zhang D, Li Q, Yan C et al (2022) Determination of intracellular lipid and main fatty acids of nannochloropsis oceanica by atr-ftir spectroscopy. J Appl Phycol. https://doi.org/10.1007/s10811-021-02607-9
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Miramontes-Corona, C., Torres-Santiago, G., Rodriguez, M.M.J. et al. Phenolic profile, antioxidant activity and antimicrobial properties of avocado (Persea americana) seed extracts. Chem. Pap. (2024). https://doi.org/10.1007/s11696-024-03452-z
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DOI: https://doi.org/10.1007/s11696-024-03452-z