Abstract
Anthocyanins occur naturally in many blue-, purple- and red-colored fruits and vegetables, and are commonly found in the human diet as natural colorants with proven health benefits. This work aimed to find the most efficient solvents for the anthocyanins extraction from natural matrices (chokeberries and black carrots), able to preserve their antioxidant activity. Four different acidified solvents (methanol, ethanol, acetone, and water) were tested and the extracts were characterized by UV–Vis spectroscopy and High-Performance Liquid Chromatography coupled with Mass Spectrometry. The anthocyanins profile of each extract has been identified. Five monoglycosylated anthocyanins were found in chokeberries and ten anthocyanins (four acylated and four diglycosylated) in black carrots. The antioxidant activities of all extracts (using ABTS, CUPRAC and FRAP assays) were determined concomitantly and ranked. The most efficient extraction was obtained using ethanol and methanol, such extracts showing the highest antioxidant activity for both matrices (black carrots and chokeberry).
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Data availability
The data used to support the findings of this study are available from the corresponding author upon request.
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This paper was published under the frame of a national grant financed by the Romanian National Authority for Scientific Research (UEFISCDI) Grant Number PN-III-P2-2.1-PED-2016-1002, 186PED, 01/09/2017.
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Conceptualization and data interpretation of the study was done by ZD; methodology and analysis were realized by MN, ADF, IȘ; writing—original draft preparation was done by DR; writing—review and editing by DR and supervision by AP and CS. Authorship was limited to those who have contributed substantially to the work reported.
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Nistor, M., Diaconeasa, Z., Frond, A.D. et al. Comparative efficiency of different solvents for the anthocyanins extraction from chokeberries and black carrots, to preserve their antioxidant activity. Chem. Pap. 75, 813–822 (2021). https://doi.org/10.1007/s11696-020-01344-6
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DOI: https://doi.org/10.1007/s11696-020-01344-6