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Ultrasound-assisted extraction of polysaccharides from brown alga (Sargassum angustifolium): structural characterization, antioxidant, and antitumor activities

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Abstract

In this study, crude polysaccharide of Sargassum angustifolium (PSA) was extracted with an extraction efficiency of 7.14% and a molecular weight of 1831.55 kDa. Then two fractions PSA-1 and PSA-2 with yields of 38.12% and 61.07% and molecular weights of 809.2 and 644.1 kDa were obtained by a DEAE column. Based on the results, fucose (40.41%), glucose (19.30%) and galactose (27.13%) were identified as the dominant monosaccharides in polysaccharides. The IC50 value of DPPH free radical scavenging by PSA, PSA-1 and PSA-2 was 0.32, 0.24 and 0.19 mg/mL, respectively. Also, the IC50 value of ABTS radical scavenging by PSA, PSA-1 and PSA-2 was estimated at about 0.24, 0.17 and 0.14 mg/mL, respectively. According to the results, Fraction PSA-2 had the highest antioxidant activity. In this study, the ability of all fractions to inhibit Hela cancer cells was determined, and the most significant effect was observed by fraction PSA-2. The inhibition rate of HeLa cells by PSA and PSA-1 and PSA-2, at a concentration of 0.4 mg/mL was about 40.56% and 51.70% and 70.81%, respectively. Therefore, S. angustifolium polysaccharide, especially fraction PSA-2, can be introduced as a natural compound with anticancer and antioxidant activity for food and pharmaceutical applications.

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Authors and Affiliations

  1. Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Akbar Norouzi, Mehdi Shamsaie Mehrgan & Houman Rajabi Islami

  2. Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran

    Laleh Roomiani

  3. Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Mehdi Raissy

Authors
  1. Akbar Norouzi
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  2. Mehdi Shamsaie Mehrgan
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Correspondence to Mehdi Shamsaie Mehrgan or Laleh Roomiani.

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Norouzi, A., Mehrgan, M.S., Roomiani, L. et al. Ultrasound-assisted extraction of polysaccharides from brown alga (Sargassum angustifolium): structural characterization, antioxidant, and antitumor activities. Food Measure 17, 6330–6340 (2023). https://doi.org/10.1007/s11694-023-02113-1

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