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Preparation, analysis and activity of Malus prunifolia polysaccharide

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Abstract

Malus prunifolia polysaccharides have the important application prospect. Crude Malus prunifolia polysaccharides were extracted. The refined polysaccharides were obtained by protein removal and dialysis, and then purified by DEAE-52 anion exchange column and Sephadex G-100 column in turn to obtain the homogeneous polysaccharide. Results showed that the Malus prunifolia polysaccharide, with Mw of 6.9 × 104 Da and → 4)-α-d-Galp (1 → 4)-α-d-Glcp (1 → glycosidic bond, was a heterosaccharide composed of arabose, galactose, glucose, xylose and galacturonic acid. It showed that the Malus prunifolia polysaccharide and its derivatives had a high potential in scavenging DPPH radical, hydroxyl radical, superoxide anion and reducing ability. The results of activity in vivo showed that the results of antioxidant activity showed that the polysaccharide and its derivatives could significantly increase the activity of SOD in mice and significantly reduce the content of MDA in mouse liver tissue. This study provided the reference for further study and rational development of Malus prunifolia polysaccharides.

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References

  1. M. Motshakeri, H.M. Ghazali, Nutritional, phytochemical and commercial quality of Noni fruit: a multi-beneficial gift from nature. Trends Food Sci. Technol. 45, 118–129 (2015)

    Article  CAS  Google Scholar 

  2. A. Ahmad, M.K. Alkharfy, T.A. Wani, M. Raish, Application of Box-Behnken design for ultrasonic-assisted extraction of polysaccharides from Paeonia emodi. Int. J. Biol. Macromol. 72, 990–997 (2015)

    Article  CAS  PubMed  Google Scholar 

  3. C. Chen, L.J. You, A.M. Abbasi, X. Fu, R.H. Liu, Optimization for ultrasound extraction of polysaccharides from Mulberry fruits with antioxidant and hyperglycemic activity in vitro. Carbohydr. Polym. 130, 122–132 (2015)

    Article  CAS  PubMed  Google Scholar 

  4. B. Ren, C. Chen, C. Li, X. Fu, L. You, R.H. Liu, Optimization of microwave-assisted extraction of, Sargassum thunbergii, polysaccharides and its antioxidant and hypoglycemic activities. Carbohydr. Polym. 173, 192–201 (2017)

    Article  CAS  PubMed  Google Scholar 

  5. Y.F. Zhang, J. Yang, Y.H. Tang, B. Tao, Z. Jiang, Establishment of mouse neuron and microglial cell co-cultured models and its action mechanism. Oncotarget 8, 43061–43067 (2017)

    Article  PubMed  PubMed Central  Google Scholar 

  6. Y.Q. Xu, Y.K. Gao, F. Liu, X.J. Niu, L.B. Wang, X.G. Li, Sulfated modification of the polysaccharides from Blackcurrant and their antioxidant and α-amylase inhibitory activities. Int. J. Biol. Macromol. 109, 1344–1354 (2018)

    Article  CAS  PubMed  Google Scholar 

  7. M. Beigi, K. Jahanbin, A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: extraction, purification and structural eatures. Int. J. Biol. Macromol. 128, 648–654 (2019)

    Article  CAS  PubMed  Google Scholar 

  8. Y. Yang, Z.X. Lei, M.M. Zhao, C.Z. Wu, L.B. Wang, Y.Q. Xu, Microwave assisted extraction of an acidic polysaccharide from Ribes nigrum L.: structural characteristics and biological activities. Ind. Crop. Prod. 147, 112249 (2020)

    Article  CAS  Google Scholar 

  9. W. Tu, J. Zhu, S. Bi, D. Chen, L. Song, L. Wang, J. Zi, R. Yu, Isolation, characterization and bioactivities of a new polysaccharide from Annona squamosa and its sulfated derivative. Carbohydr. Polym. 152, 287–296 (2016)

    Article  CAS  PubMed  Google Scholar 

  10. W. Liu, Y. Liu, R. Zhu, J. Yu, W. Lu, C. Pan, W. Yao, X. Gao, Structure characterization, chemical and enzymatic degradation, and chain conformation of an acidic polysaccharide from Lycium barbarum L. Carbohydr. Polym. 147, 114–124 (2016)

    Article  CAS  PubMed  Google Scholar 

  11. Z. Zhang, F. Kong, H. Ni, Z. Mo, J.B. Wan, D. Hua, C. Yan, Structural characterization, α-glucosidase inhibitory and DPPH scavenging activities of polysaccharides from Guava. Carbohydr. Polym. 144, 106–114 (2016)

    Article  CAS  PubMed  Google Scholar 

  12. Z. Tang, Y. Wang, G. Huang, H. Huang, Ultrasound-assisted extraction, analysis and antioxidant activity of polysaccharide from the rinds of Garcinia mangostana L. Ultrason. Sonochem. 97, 106474 (2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. M.R. Ajami, A. Ganjloo, M. Bimakr, Continuous fast microwave-assisted extraction of radish leaves polysaccharides: optimization, preliminary characterization, biological, and techno-functional properties. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03522-w

    Article  Google Scholar 

  14. Y. Fan, G. Huang, Preparation, structural analysis and antioxidant activity of polysaccharides and their derivatives from Pueraria lobata. Chem. Biodivers. 20(2023)

    Article  CAS  PubMed  Google Scholar 

  15. B. Li, G. Huang, Preparation, structure-function relationship and application of Grifola umbellate polysaccharides. Ind. Crops Prod. 186(2022)

    Article  CAS  Google Scholar 

  16. S. Zhou, G. Huang, Extraction, structural analysis and antioxidant activity of aloe polysaccharide. J. Mol. Struct. 1273(2023)

    Article  CAS  Google Scholar 

  17. S. Zhou, G. Huang, G. Chen, Extraction, structural analysis, derivatization and antioxidant activity of polysaccharide from Chinese yam. Food Chem. 361(2021)

    Article  CAS  PubMed  Google Scholar 

  18. J. Li, H. Shi, J. Yu, Y. Lei, G. Huang, H. Huang, Extraction and properties of Ginkgo biloba leaf polysaccharide and its phosphorylated derivative. Ind. Crops Prod. 189(2022)

    Article  CAS  Google Scholar 

  19. Y. Wang, X. Xiong, G.L. Huang, Ultrasound-assisted extraction and analysis of maidenhairtree polysaccharides. Ultrason. Sonochem. 95(2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Z. Song, X. Xiong, G. Huang, Ultrasound-assisted extraction and characteristics of maize polysaccharides from different sites. Ultrason. Sonochem. 95(2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. J. Li, Y. Fan, G. Huang, H. Huang, Extraction, structural characteristics and activities of Zizylphus vulgaris polysaccharides. Ind. Crops Prod. 178(2022)

    Article  Google Scholar 

  22. S. Zhou, G. Huang, H. Huang, Extraction, derivatization and antioxidant activities of onion polysaccharide. Food Chem. 388(2022)

    Article  CAS  PubMed  Google Scholar 

  23. J. Li, Z. Chen, H. Shi, J. Yu, G. Huang, H. Huang, Ultrasound-assisted extraction and properties of polysaccharide from Ginkgo biloba leaves. Ultrason. Sonochem. 93(2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. X. Xiong, W. Yang, G. Huang, H. Huang, Ultrasonic-assisted extraction, characteristics and activity of Ipomoea batatas polysaccharide. Ultrason. Sonochem. 96(2023)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. W. Yang, G.L. Huang, Chemical modification and structural analysis of polysaccharide from Solanum tuberdsm. J. Mol. Struct. 1285(2023)

    Article  CAS  Google Scholar 

  26. W. Yang, G. Huang, F. Chen, H. Huang, Extraction/synthesis and biological activities of selenopolysaccharide. Trends Food Sci. Technol. 109, 211–218 (2021)

    Article  CAS  Google Scholar 

  27. W. Yang, G. Huang, Extraction methods and activities of natural glucans. Trends Food Sci. Technol. 112, 50–57 (2021)

    Article  CAS  Google Scholar 

  28. F. Chen, G. Huang, H. Huang, Preparation, analysis, antioxidant activities in vivo of phosphorylated polysaccharide from Momordica charantia. Carbohydr. Polym. 252(2021)

    Article  CAS  PubMed  Google Scholar 

  29. X. Mei, Q. Tang, G. Huang, R. Long, H. Huang, Preparation, structural analysis and antioxidant activities of phosphorylated (1→3)-β-d-glucan. Food Chem. 309(2020)

    Article  CAS  PubMed  Google Scholar 

  30. B. Lin, G. Huang, Extraction, isolation, purification, derivatization, bioactivity, structure-activity relationship, and application of polysaccharides from White jellyfungus. Biotechnol. Bioeng. 119(6), 1359–1379 (2022)

    Article  CAS  PubMed  Google Scholar 

  31. B. Lin, G. Huang, An important polysaccharide from fermentum. Food Chem. 15(2022)

    CAS  Google Scholar 

  32. Z. Tang, G. Huang, Extraction, structure, and activity of polysaccharide from Radix astragali. Biomed. Pharmacother. 150(2022)

    Article  CAS  PubMed  Google Scholar 

  33. Q. Tang, G. Huang, Improving method, properties and application of polysaccharide as emulsifier. Food Chem. 376(2022)

    Article  CAS  Google Scholar 

  34. S. Zhou, G. Huang, The inhibitory activity of natural products to xanthine oxidase. Chem. Biodivers 20(2023)

    Article  CAS  PubMed  Google Scholar 

  35. W. Zhang, G. Huang, Preparation, structural characteristics, and application of taro polysaccharides in food. J. Sci. Food Agric. 102, 6193–6201 (2022)

    Article  CAS  PubMed  Google Scholar 

  36. Q. Tang, G. Huang, F. Zhao, L. Zhou, S. Huang, H. Li, The antioxidant activities of six (1→3)-β-d-glucan derivatives prepared from yeast cell wall. Int. J. Biol. Macromol. 98, 216–221 (2017)

    Article  CAS  PubMed  Google Scholar 

  37. G. Huang, F. Chen, W. Yang, H. Huang, Preparation, deproteinization and comparison of bioactive polysaccharides. Trends Food Sci. Technol. 109, 564–568 (2021)

    Article  CAS  Google Scholar 

  38. G. Benchamas, G. Huang, S. Huang, H. Huang, Preparation and biological activities of chitosan oligosaccharides. Trends Food Sci. Technol. 107, 38–44 (2021)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Carbohydrate Science and Engineering, College of Chemistry, Chongqing Normal University, Chongqing, 401331, China

    Shiyang Zhou & Gangliang Huang

  2. Chongqing Chemical Industry Vocational College, Chongqing, 401228, China

    Shiyang Zhou

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  1. Shiyang Zhou
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Zhou, S., Huang, G. Preparation, analysis and activity of Malus prunifolia polysaccharide. Food Measure 17, 4963–4979 (2023). https://doi.org/10.1007/s11694-023-01982-w

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