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Purification and identification of dual-enzyme hydrolysates obtained from defatted walnut and its antioxidant effects on d-galactose-induced aging mice

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Abstract

The purpose of this study was to explore the antioxidant activity in vivo of dual-enzyme hydrolysates obtained from defatted walnut, and to purify and identify it. The walnut protein hydrolysates (WPH) were obtained through two-step enzymatic hydrolysis of alkaline protease and trypsin. The effects of WPH on the oxidative stress-related biochemical indexes of d-galactose-treated mice were investigated. Next, WPH was passed through a Sephadex G-25 gel chromatography, anion exchange chromatography and Sephadex G-15 gel chromatography. Subsequently, peptides in the high antioxidant activity fraction were identified by RP-nano-HPLC–MS/MS. Finally, 14 potential peptides of length 11 AAs to 21 AAs with antioxidant activity were identified from WPH.

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We declared that materials described in the manuscript, including all relevant raw data, will be freely available to any scientist wishing to use them for non-commercial purposes, without breaching participant confidentiality.

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Funding

This work was supported by the National Natural Science Foundation of China under Grant [No. 31801568]; the Natural Science Foundation of Tianjin city of China under Grant [No. 18JCQNJC79300].

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, No. 29, 13th Road, Tianjin Economic and Technical Development Zone, Tianjin, 300457, People’s Republic of China

    Qi Jia, Jin-Fang Yuan, Hui-Ping Liu, Meng-Yu Li & Ya-Ru Wu

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  1. Qi Jia
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  2. Jin-Fang Yuan
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  3. Hui-Ping Liu
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  4. Meng-Yu Li
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Correspondence to Hui-Ping Liu.

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We declare that we have no conflict of interest exists in the submission of this manuscript, and we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted. Manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that encloses.

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All animal experimental procedures were conducted according to the principles of Laboratory Animal Care as approved by the Local Ethics Committee for Animal Care and Use at Tianjin University of Science and Technology.

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Jia, Q., Yuan, JF., Liu, HP. et al. Purification and identification of dual-enzyme hydrolysates obtained from defatted walnut and its antioxidant effects on d-galactose-induced aging mice. Food Measure 15, 1034–1043 (2021). https://doi.org/10.1007/s11694-020-00702-y

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  • DOI: https://doi.org/10.1007/s11694-020-00702-y

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