Abstract
Ultrasound assisted extraction (UAE) technique was employed for the extraction of phytochemicals from Coccinia indica fruit. Twenty-two compounds were identified post extraction process by GCMS analysis. Temperature, time of extraction, solvent concentration and solid-solvent (SS) ratio were considered as important process parameters for UAE. Response surface method was applied for optimizing the extraction process. The study focussed on the yield of extract, antioxidant activity (DPPH and ABTS), total phenolic content and total flavonoid content as the dependent variables. The optimum values of process parameters were obtained to be 30 °C temperature, 42 min of extraction time, 63% solvent (ethanol) concentration and SS ratio of 1:60. At these optimum conditions, the extract yield was 18.58%. The DPPH activity of 83.42%, 27.18% ABTS radical scavenging ability, 12.82 mg Gallic acid equivalent (GAE)/g of phenolic content and 79.81 mg Rutin equivalent (RE)/g of flavonoid content were obtained. Major compounds identified by GCMS were rutin, naringenin, apigenin and kaempferol. Resveratrol was also identified in the extract of C. indica fruits.
Similar content being viewed by others
References
C. Kalpanadevi, V. Singh, R. Subramanian, J. Food Sci. Technol. 55, 2259 (2018)
M. Farahmand, M.T. Golmakani, G. Mesbahi, A. Farahnaky, J. Food Process. Preserv. 41, 1 (2017)
E. Wasantwisut, T. Viriyapanich, World Rev. Nutr. Diet. 91, 60 (2003)
M. Kumar, S. Alok, S.K. Jain, V.K. Dixit, Asian Paci. J. Trop. Dis. 4, S121 (2014)
J.H. Doughari, Phytochemicals: A Global Perspective of Their Role in Nutrition and Health (InTech, Rijeka, 2012)
F. Chemat, N. Rombaut, A.G. Sicaire, A. Meullemiestre, A.S. Fabiano-Tixier, M. Abert-Vian, Ultrason. Sonochem. 34, 540 (2017)
P. Garcia-Salas, A. Morales-Soto, A. Segura-Carretero, A. Fernández-Gutiérrez, Molecules 15, 8813 (2010)
M.M.A. Rashed, Q. Tong, M.H. Abdelhai, M.A.A. Gasmalla, J.B. Ndayishimiye, L. Chen, F. Ren, Ultrason. Sonochem. 29, 39 (2016)
Z. Füzfai, I. Molnár-Perl, J. Chromatogr. A 1149, 88 (2007)
Á. Guinda, J.M. Castellano, J.M. Santos-Lozano, T. Delgado-Hervás, P. Gutiérrez-Adánez, M. Rada, LWT - Food Sci. Technol. 64, 431 (2015)
L. Degani, C. Riedo, M. Gulmini, O. Chiantore, Chromatographia 77, 1683 (2014)
T. Belwal, I.D. Bhatt, R.S. Rawal, V. Pande, Ind. Crops Prod. 95, 393 (2017)
N.A. Al-Dhabi, K. Ponmurugan, P. Maran Jeganathan, Ultrason. Sonochem. 34, 206 (2017)
H. Shirzad, V. Niknam, M. Taheri, H. Ebrahimzadeh, J. Food Sci. Technol. 54, 2361 (2017)
S. Chen, Z. Zeng, N. Hu, B. Bai, H. Wang, Y. Suo, Food Chem. 242, 1 (2018)
R.E. Ghitescu, I. Volf, C. Carausu, A.M. Bühlmann, I.A. Gilca, V.I. Popa, Ultrason. Sonochem. 22, 535 (2015)
S. Rodrigues, F.A.N. Fernandes, E.S. de Brito, A.D. Sousa, N. Narain, Ind. Crops Prod. 69, 400 (2015)
J. Wang, B. Sun, Y. Cao, Y. Tian, X. Li, Food Chem. 106, 804 (2008)
L. Montanari, P. Fantozzi, J.M. Snyder, J.W. King, J. Supercrit. Fluids 14, 87 (1999)
H.D.F.Q. Barros, A.M. Baseggio, C.F.F. Angolini, G.M. Pastore, C.B.B. Cazarin, M.R. Marostica-Junior, Food Res. Int. 124, 16 (2019)
A.B. Das, V.V. Goud, C. Das, Ind. Crops Prod. 95, 332 (2017)
S.A. Baba, S.A. Malik, J. Taibah Univ. Sci. 9, 449 (2015)
O.R. Alara, N.H. Abdurahman, C.I. Ukaegbu, J. Appl. Res. Med. Aromat. Plants 11, 12 (2018)
C. Proestos, D. Sereli, M. Komaitis, Food Chem. 95, 44 (2006)
H. Zhang, J. Birch, C. Xie, H. Yang, A. El-Din Bekhit, Food Chem. 294, 276 (2019)
N. Medina-Torres, T. Ayora-Talavera, H. Espinosa-Andrews, A. Sánchez-Contreras, N. Pacheco, Agronomy 7, 47 (2017)
A. Figueiras Abdala, N. Mendoza, N. Valadez Bustos, E.M. Escamilla Silva, Plant Foods Hum. Nutr. 72, 258 (2017)
A. Ali, X.Y. Lim, C.H. Chong, S.H. Mah, B.L. Chua, LWT - Food Sci. Technol. 89, 681 (2018)
J. Sang, Q. Ma, B. Li, C.Q. Li, J. Food Meas. Charact. 12, 45 (2018)
J. Živković, K. Šavikin, T. Janković, N. Ćujić, N. Menković, Sep. Purif. Technol. 194, 40 (2018)
Z. Zhao, M.H. Moghadasian, Food Chem. 109, 691 (2008)
W. Huang, A. Xue, H. Niu, Z. Jia, J. Wang, Food Chem. 114, 1147 (2009)
C. Proestos, M. Komaitis, Foods 2, 90 (2013)
B. Gullón, T.A. Lú-Chau, M.T. Moreira, J.M. Lema, G. Eibes, Trends Food Sci. Technol. 67, 220 (2017)
J.M. Calderon-Montano, E. Burgos-Moron, C. Perez-Guerrero, M. Lopez-Lazaro, Mini-Rev. Med. Chem. 11, 298 (2011)
F. Ali, Rahul, F. Naz, S. Jyoti, Y.H. Siddique, Int. J. Food Prop. 20, 1197 (2017)
B. Salehi, P.V.T. Fokou, M. Sharifi-Rad, P. Zucca, R. Pezzani, N. Martins, J. Sharifi-Rad, Pharmaceuticals 12, 1 (2019)
E. Rezaeiamiri, R. Bahramsoltani, R. Rahimi, Clin. Nutr. 39, 331 (2020)
W. Wang, C. Sun, L. Mao, P. Ma, F. Liu, J. Yang, Y. Gao, Trends Food Sci. Technol. 56, 21 (2016)
M. Imran, A. Rauf, T. Abu-Izneid, M. Nadeem, M.A. Shariati, I.A. Khan, A. Imran, I.E. Orhan, M. Rizwan, M. Atif, T.A. Gondal, M.S. Mubarak, Biomed. Pharmacother. 112, 108612 (2019)
B. Sultana, F. Anwar, Food Chem. 108, 879 (2008)
M.N. Clifford, A. Scalbert, J. Sci. Food Agric. 80, 1118 (2000)
N. Nićiforović, H. Abramovič, Compr. Rev. Food Sci. Food Saf. 13, 34 (2014)
K. Pei, J. Ou, J. Huang, S. Ou, J. Sci. Food Agric. 96, 2952 (2016)
K. Gul, A. Tak, A.K. Singh, P. Singh, B. Yousuf, A.A. Wani, Cogent Food Agric. 1, 1 (2015)
S. Saeidnia, Eur. J. Med. Plants 4, 590 (2014)
S. Wang, M. Bilal, H. Hu, W. Wang, X. Zhang, Appl. Microbiol. Biotechnol. 102, 3561 (2018)
R. Cai, X. Li, B. Chen, Y. Xie, H. Xie, D. Chen, ChemistrySelect 4, 5155 (2019)
B. Šiler, D. Mišić, in Studies in Natural Products Chemistry, ed. by Atta-ur-Rahman (Elsevier Science Publishers, 2016), p. 363
I.I. Abdallah, W.J. Quax, KnE Life Sci. 3, 81 (2017)
A. Sharma, B. Mazumdar, A. Keshav, Int. J. Chem. Mol. Eng. 13, 20 (2019)
Funding
This research was not supported by any kind of financial aid.
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Sharma, A., Mazumdar, B. & Keshav, A. Ultrasound assisted extraction of phytochemicals from Coccinia indica fruits and its characterization. Food Measure 15, 466–477 (2021). https://doi.org/10.1007/s11694-020-00633-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11694-020-00633-8