Abstract
Peptides from natural materials have been reported owning potential for regulating oxidative stress and hyperglycemia for human body. In this study, peptides from a favorite food, Porphyra haitanensis, were prepared by consecutive procedures including ultrasound-assisted extraction, in vitro simulated gastrointestinal digestion and fraction. Subsequently, their relevant bioactivities were investigated by several in vitro methods, such as 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and α-glucosidase enzyme inhibition assays. By single factor design experiment, the highest yield of crude protein was 3.42% extracted by alkaline solution for 20 min sonication at 35 °C. After digestion, peptides were mainly distributed in F2–F11 fractions by sephadex G-25, and the peak value was found in F5. The significant activities in three assays were displayed in F3–F7. As calculated by the polynomial curve fitting, a significantly positive correlation was found between the peptide contents and the DPPH radical scavenging rate, the FRAP value, and the α-glucosidase enzyme inhibitory rate (R2 = 0.8490, R2 = 0.7579, R2 = 0.9701, respectively). The results showed that these peptides possessed promising antioxidant activity and inhibitory activity on α-glucosidase. Therefore, peptides derived from P. haitanensis have potential anti-diabetic applications in pharmaceutical and functional food industries.
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Acknowledgements
This work was supported by Medical Fund Project of Guangdong Province (A2018224), Science and Technology Plan Project of Zhanjiang (2018B01001), the Innovative Experimental Project for College Students of Guangdong Medical University (2KY17018), Grants from the Key Laboratories Project of Universities in Guangdong Province (2015KTSCX052), Provincial-Municipal Co-construction Projects of Guangdong Province (4SG18102G, 4SG18004G, 4SG18056G, 4SG17043), and grants from the Dongguan Key Laboratory of Environmental Medicine, Funds for PHD researchers of Guangdong Medical University in 2019 (B2019001).
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Wen, L., Tan, S., Zeng, L. et al. Ultrasound-assisted extraction and in vitro simulated digestion of Porphyra haitanensis proteins exhibiting antioxidative and α-glucosidase inhibitory activity. Food Measure 14, 3291–3298 (2020). https://doi.org/10.1007/s11694-020-00564-4
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DOI: https://doi.org/10.1007/s11694-020-00564-4