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Optimization of ultrasonic-assisted extraction, refinement and characterization of water-soluble polysaccharide from Dictyosphaerium sp. and evaluation of antioxidant activity in vitro

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Abstract

Microalgae have been reported in many studies with high biomass, high carbohydrate content, fast growth rate and multiple bio-activities, while Dictyosphaerium sp. was rarely studied. Polysaccharide prove to be remarkable in many biological activities. To optimize the effects of the ultrasonic-assisted extraction parameters on the yield of polysaccharide from Dictyosphaerium sp., a single-factor experiment and response surface methodology (RSM) with a central composite rotatable design were employed. Four independent variables were investigated: X1 (ratio of raw microalgal powder to water), X2 (extraction temperature), X3 (extraction time), X4 (extraction power). The optimal conditions obtained via single-factor experiment were: X1 (1:25), X2 (50 °C), X3 (50 min), X4 (500 W), while according to the analysis of RSM, the optimum conditions were: X1 (1:26), X2 (58 °C), X3 (55 min), X4 (525 W). The refined polysaccharide were further prepared and characterized using SEM, FT-IR, XRD and TGA/DSC. Five kinds of antioxidant assays were carried out and the results indicated that the refined polysaccharide had strong scavenging ability to DPPH· and OH·. This study provides theoretical and experimental basis for the development and utilization of microalgal resources.

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Acknowledgements

The present article is supported by Hainan Provincial Natural Science Foundation of China (219QN258), Hainan Provincial Scientific Research Program of Higher Education Institutions for China (Hnky2019-110). Zhenyu Zhao acknowledges the China Scholarship Council for financial support.

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  1. Chen Chen and Zhenyu Zhao have contribute equally to this study and joint co-first authors.

Authors and Affiliations

  1. Hainan Health Management College, Chengmai County, 571900, Hainan, China

    Chen Chen, Shasha Ma, Le Wang & Jie Zhang

  2. Centre for Surface Chemistry and Catalysis, Faculty of Bio-Science Engineering, KU Leuven, Celestijnenlaan 200F, PO Box 2461, 3001, Louvain, Belgium

    Zhenyu Zhao & Muhammad Azam Rasool

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  1. Chen Chen
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Chen, C., Zhao, Z., Ma, S. et al. Optimization of ultrasonic-assisted extraction, refinement and characterization of water-soluble polysaccharide from Dictyosphaerium sp. and evaluation of antioxidant activity in vitro. Food Measure 14, 963–977 (2020). https://doi.org/10.1007/s11694-019-00346-7

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