Skip to main content
SpringerLink
Log in

Quantification and monitoring the heat-induced formation of trans fatty acids in edible oils by Raman Spectroscopy

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

Trans fatty acids (TFAs), a type of unsaturated fatty acids, has been linked to increased risks of coronary heart disease and other health problems. Thus, efficient and reliable detection methods for TFAs are of great importance for both industrial applications and research purposes. In this study, a rapid and pretreatment-free quantification approach was developed for TFAs in 13 edible oils (including 2 soybean oils, 2 peanut oils, 2 rapeseed oils, blend oil, oleum camelliae, sunflower oil, rice oil, sesame oil, olive oil and corn oil) with Raman spectroscopy and chemometric methods. The commercial oils contained 0–0.397% (w/w) of trielaidin and each of the oils were further added with 0.1–5% of trielaidin, resulting in a total of 130 samples. Results show that the concentration of TFAs in various oils can be well-predicted based on the Raman spectral features in the region of 1640–1680 cm−1 (characteristic to C=C stretching modes), yielding a residual predictive deviation (RPD) of 4.639. This approach exhibits high sensitivity in quantification, and TFAs added in different kinds of edible oils combined with their initial levels can be successfully quantified with good descriptive statistics (Rc = 0.9657, Rcv = 0.9598, Rp = 0.9634, RMSEC = 0.351, RMSECV = 0.391 and RMSEP = 0.302). Furthermore, in order to understand the heat-induced formation of TFAs during food preparation, the TFA concentrations of the oil heated under different temperatures was investigated based on the above predication model. The results indicate that the concentration of TFAs increased sharply when the oils were heated at 169–250 °C for 10 min.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. T.G. Albuquerque, H.S. Costa, M.C. Castilho, A. Sanches-Silva, Trends Food Sci. Technol. 22, 543 (2011)

    Article  CAS  Google Scholar 

  2. T.L.A. Prado, M.A.L. Oliveira, Anal. Methods 9, 2483 (2017)

    Article  CAS  Google Scholar 

  3. R. L. Wolff, D. Precht, and J. Molkentin, Trans Fat. Acids Hum. Nutr. 1 (1998).

  4. L. Brühl, Eur. J. Lipid Sci. Technol. 116, 707 (2014)

    Article  Google Scholar 

  5. M.Á. Carmona, F. Lafont, C. Jiménez-Sanchidrián, J.R. Ruiz, Eur. J. Lipid Sci. Technol. 116, 1451 (2014)

    Article  CAS  Google Scholar 

  6. A. Grandgirard, J.L. Sebedio, J. Fleury, J. Am. Oil Chem. Soc. 61, 1563 (1984)

    Article  CAS  Google Scholar 

  7. W. Tsuzuki, A. Matsuoka, K. Ushida, Food Chem. 123, 976 (2010)

    Article  CAS  Google Scholar 

  8. R.K. Johnson, A.E. Black, T.J. Cole, N. Engl, J. Med. 338, 918 (1998)

    CAS  Google Scholar 

  9. J. Salmerón, F.B. Hu, J.E. Manson, M.J. Stampfer, G.A. Colditz, E.B. Rimm, W.C. Willett, Am. J. Clin. Nutr. 73, 1019 (2001)

    Article  Google Scholar 

  10. S.E. Carlson, M.T. Clandinin, H.W. Cook, E.A. Emken, L.J. Filer, Am. J. Clin. Nutr. 66, 717S (1997)

    Article  Google Scholar 

  11. FDA, (2010).

  12. A. Astrup, 7, 43 (2006).

  13. M. Zhang, X. Yang, H.T. Zhao, A.J. Dong, J. Wang, G.Y. Liu, P. Wang, C.L. Cheng, H. Zhang, Food Control 57, 293 (2015)

    Article  CAS  Google Scholar 

  14. W. Tsuzuki, K. Ushida, Lipids 44, 373 (2009)

    Article  CAS  Google Scholar 

  15. J. Kraft, J.K.G. Kramer, M. Hernandez, J. Letarte, N. Aldai, V. Sandercole, R. Mohammed, F. Mayer, M.M. Mossoba, P. Delmonte, Lipid Technol. 26, 39 (2014)

    Article  CAS  Google Scholar 

  16. G.F. Bailey, R.J. Horvat, J. Am. Oil Chem. Soc. 49, 494 (1972)

    Article  CAS  Google Scholar 

  17. C. Jiménez-Sanchidrián, J.R. Ruiz, Appl. Spectrosc. Rev. 51, 417 (2016)

    Article  Google Scholar 

  18. M. Meurens, V. Baeten, S.H. Yan, E. Mignolet, Y. Larondelle, J. Agric. Food Chem. 53, 5831 (2005)

    Article  CAS  Google Scholar 

  19. I. Stefanov, V. Baeten, O. Abbas, E. Colman, B. Vlaeminck, B. De Baets, V. Fievez, J. Agric. Food Chem. 58, 10804 (2010)

    Article  CAS  Google Scholar 

  20. Y. Li, T. Fang, S. Zhu, F. Huang, Z. Chen, Y. Wang, Spectrochim. Acta. 189, 37 (2018).

  21. H. Ali, H. Nawaz, M. Saleem, F. Nurjis, M. Ahmed, J. Raman Spectrosc. 47, 706 (2016)

    Article  CAS  Google Scholar 

  22. J. Kim, J.H. Lee, D.-K. Ko, J. Raman Spectrosc. 45, 591 (2014)

    Article  CAS  Google Scholar 

  23. B. Muika, B. Lendlb, A. Molina-Díaza, M.J. Ayora-Cañada, Chem. Phys. Lipids 134, 173 (2005)

    Article  Google Scholar 

  24. D.P. Killeen, S.N. Marshall, E.J. Burgess, K.C. Gordon, N.B. Perry, J. Agric. Food Chem. 65, 3551 (2017)

    Article  CAS  Google Scholar 

  25. S.D. Velioglua, H.T. Temizb, E. Ercioğlub, H.M. Veliogluc, A. Topcub, I.H. Boyacib, Food Chem. 221, 87 (2017)

    Article  Google Scholar 

  26. S.D. Velioglu, E. Ercioglu, H.T. Temiz, H.M. Velioglu, A. Topcu, I.H. Boyaci, JAOCS. J. Am. Oil Chem. Soc. 93, 627 (2016)

    Article  CAS  Google Scholar 

  27. B. Bernuy, M. Meurens, E. Mignolet, C. Turu, Y. Larondelle, J. Agric. Food Chem. 57, 6524 (2009)

    Article  CAS  Google Scholar 

  28. I. Stefanov, V. Baeten, O. Abbas, E. Colman, B. Vlaeminck, B. De Baets, V. Fievez, J. Agric. Food Chem. 59, 12771 (2011)

    Article  CAS  Google Scholar 

  29. M. Melchiorre, C. Ferreri, A. Tinti, C. Chatgilialoglu, A. Torreggiani, Appl. Spectrosc. 69, 613 (2015)

    Article  CAS  Google Scholar 

  30. H. Li, F.R. Van de Voort, J. Sedman, A.A. Ismail, J. Am. Oil Chem. Soc. 76, 491 (1999)

    Article  CAS  Google Scholar 

  31. E. Birkel, L. Rodriguez-Saona, JAOCS. J. Am. Oil Chem. Soc. 88, 1477 (2011)

    Article  CAS  Google Scholar 

  32. Z.-Y. Deng, B. Zhang, W. Dong, X.-P. Wang, Guang Pu Xue Yu Guang Pu Fen Xi. Spectrosc. Spectr. Anal. 33, 2997 (2013)

    CAS  Google Scholar 

  33. L. Xie, Y. Ying, T. Ying, J. Agric. Food Chem. 55, 4645 (2007)

    Article  CAS  Google Scholar 

  34. L. Massart and B. Vandeginste, Handbook of Chemometrics, Part A (Elsevier Science Inc., Amsterdam, 1997).

  35. M. Zhao, R.J. Beattie, A.M. Fearon, C.P. O’Donnell, G. Downey, Int. Dairy J. 51, 41 (2015)

    Article  Google Scholar 

  36. W. Xu, L. Xie, Z. Ye, W. Gao, Y. Yao, M. Chen, J. Qin, Y. Ying, Sci. Rep. 5, 1 (2015)

    Google Scholar 

  37. M. Rahimi, E. Karimi, M. Nekoei, M. Mohammadhosseini, J. Essent. Oil-Bearing Plants 19, 307 (2016)

    Article  CAS  Google Scholar 

  38. S.R. Karunathilaka, M.M. Mossoba, J.K. Chung, E.A. Haile, C.T. Srigley, J. Agric. Food Chem. 65, 224 (2017)

    Article  CAS  Google Scholar 

  39. C. Liu, W. Liu, W. Chen, J. Yang, L. Zheng, Food Chem. 173, 482 (2015)

    Article  CAS  Google Scholar 

  40. V. Baeten, P. Hourant, M.T. Morales, R. Aparicio, J. Agric. Food Chem. 46, 2638 (1998)

    Article  CAS  Google Scholar 

  41. A. M. Miranda, E. W. Castilho-Almeida, E. H. Martins Ferreira, G. F. Moreira, C. A. Achete, R. A. S. Z. Armond, H. F. Dos Santos, A. Jorio, Fuel 115, 118 (2014).

    Article  CAS  Google Scholar 

  42. Y.M. Weng, R.H. Weng, C.Y. Tzeng, W. Chen, Appl. Spectrosc. 57, 413 (2003)

    Article  CAS  Google Scholar 

  43. Y. Chen, Y. Yang, S. Nie, X. Yang, Y. Wang, M. Yang, C. Li, M. Xie, Food Control 44, 191 (2014)

    Article  CAS  Google Scholar 

  44. M. Yang, Y. Yang, S. Nie, M. Xie, F. Chen, JAOCS. J. Am. Oil Chem. Soc. 89, 859 (2012)

    Article  CAS  Google Scholar 

  45. C. Li, Y. Zhang, S. Li, G. Wang, C. Xu, Y. Deng, S. Wang, J. Agric. Food Chem. 61, 10392 (2013)

    Article  CAS  Google Scholar 

  46. S. Bhardwaj, S.J. Passi, A. Misra, K.K. Pant, K. Anwar, R.M. Pandey, V. Kardam, Food Chem. 212, 663 (2016)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the financial support of National key R&D plan of China (2017YFC1601700) and Natural Sciences Fund of Zhejiang Province (No. LY17C130005). We all appreciate the experimental equipment support provided by Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture, P. R. China.

Author information

Authors and Affiliations

  1. College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China

    Weichao Gong, Ruyi Shi, Min Chen, Jianyuan Qin & Xiangjiang Liu

Authors
  1. Weichao Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruyi Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Min Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianyuan Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiangjiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiangjiang Liu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human or animal subjects.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gong, W., Shi, R., Chen, M. et al. Quantification and monitoring the heat-induced formation of trans fatty acids in edible oils by Raman Spectroscopy. Food Measure 13, 2203–2210 (2019). https://doi.org/10.1007/s11694-019-00140-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-019-00140-5

Keywords

Search

Navigation