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A theoretical and experimental study: the influence of different standards on the determination of total phenol content in the Folin–Ciocalteu assay

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Abstract

With the aim of choosing an appropriate standard for determining total phenol content (TPC) in food extracts, a theoretical study was done to demonstrate the electronic properties of nine phenolic compounds. Besides, TPC of three different tea extracts was determined by the Folin–Ciocalteu (F–C) assay with nine phenolic compounds as the standards. The frontier molecular orbitals (FMO), molecular electrostatic potential (MEP) and ionization potential (IP) of these standards were calculated with density functional theory. Results indicated the active sites of the nine standards by FMO and MEP. Moreover, the IP value of epigallocatechin gallate was about 15% lower than that of epigallocatechin, indicating that the 3-galloy group at C ring rendered a higher reactivity in the F–C assay. TPC of green tea measured by epicatechin was about 19% lower than that of gallic acid, suggesting that epicatechin was not an appropriate standard for tea extracts. It is deduced that gallic acid is a comparably good standard among commercial standards (relative standards). However, it is recommended that researchers should not choose a universal standard for all food extracts considering the heterogeneity and diversity of polyphenols in food extracts.

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References

  1. C. Fernando, A.L. Cristina, T. Sara, J.T. Francisco, I.Q.O. María, J. Nutr. Biochem. 24, 1415–1422 (2013)

    Article  CAS  Google Scholar 

  2. R.D. Del, L.G. Costa, M.E. Lean, A. Crozier, Nutr. Metab. Cardiovasc. Dis. 20, 1–6 (2010)

    Article  CAS  Google Scholar 

  3. Y. Zhou, J. Zheng, Y. Li, D.P. Xu, S. Li, Y.M. Chen, H.B. Li, Nutrients 8, E515 (2016)

    Article  CAS  PubMed  Google Scholar 

  4. J. Dai, R.J. Mumper, Molecules 15, 7313–7352 (2010)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. H. Lee, M.J. Ha, H.M. Shahbaz, J.U. Kim, H. Jang, J. Park, J. Food Eng. 238, 141–147 (2018)

    Article  CAS  Google Scholar 

  6. P. Pajak, R. Socha, J. Broniek, K. Krolikowska, T. Fortuna, Food Chem. 275, 69–76 (2019)

    Article  CAS  PubMed  Google Scholar 

  7. I. Ramirez-Sanchez, L. Maya, G. Ceballos, F. Villarreal, J. Food Compos. Anal. 23, 790–793 (2010)

    Article  CAS  Google Scholar 

  8. G. Daniel, S.S. Janio, G.M. Laercio, S.N. Domingos, TrAC Trends Anal. Chem. 80, 266–279 (2016)

    Article  CAS  Google Scholar 

  9. B. Andressa, C.L. Gisely, C.P.M. Joao, Molecules 18, 6825–6856 (2013)

    Google Scholar 

  10. J.C. Sánchezrangel, J. Benavides, J.B. Heredia, L. Cisneroszevallos, D.A. Jacobovelázquez, Anal. Methods 5, 5990–5999 (2013)

    Article  CAS  Google Scholar 

  11. V. Deepha, R. Praveena, R. Sivakumar, K. Sadasivam, Spectrochim. Acta 121, 737–745 (2014)

    Article  CAS  Google Scholar 

  12. A.E. Hagerman, L.G. Butler, J. Chem. Ecol. 15, 1795–1810 (1989)

    Article  CAS  PubMed  Google Scholar 

  13. C.S. Wisdom, A. Gonzalez-Coloma, P.W. Rundel, Oecologia 72, 395–401 (1987)

    Article  CAS  PubMed  Google Scholar 

  14. A.D. Becke, J. Chem. Phys. 98, 5648 (1993)

    Article  CAS  Google Scholar 

  15. C. Lee, W. Yang, R.G. Parr, Phys. Rev. B 37, 785–789 (1988)

    Article  CAS  Google Scholar 

  16. R. Ditchfield, W.J. Hehre, J.A. Pople, J. Chem. Phys. 54, 724–728 (1971)

    Article  CAS  Google Scholar 

  17. W.J. Hehre, R. Ditchfield, J.A. Pople, J. Chem. Phys. 56, 2257–2261 (1972)

    Article  CAS  Google Scholar 

  18. Y.Z. Rong, Z.W. Wang, B. Zhao, Food Biophys. 8, 90–94 (2013)

    Article  Google Scholar 

  19. M.J. Frisch, G.W. Trucks, H.B. Schlegel, G.E. Scuseria, M.A. Robb, J.R. Cheeseman, J.A. Montgomery, T. Vreven, K.N. Kudin, J.C. Burant, J.M. Millam, S.S. Iyengar, J. Tomasi, V. Barone, B. Mennucci, M. Cossi, G. Scalmani, N. Rega, G.A. Petersson, H. Nakatsuji, M. Hada, M. Ehara, K. Toyota, R. Fukuda, J. Hasegawa, M. Ishida, T. Nakajima, Y. Honda, O. Kitao, H. Nakai, M. Klene, X. Li, J.E. Knox, H.P. Hratchian, J.B. Cross, C. Adamo, J. Jaramillo, R. Gomperts, R.E. Stratmann, O. Yazyev, A.J. Austin, R. Cammi, C. Pomelli, J.W. Ochterski, P.Y. Ayala, K. Morokuma, G.A. Voth, P. Salvador, J.J. Dannenberg, V.G. Zakrzewski, S. Dapprich, A.D. Daniels, M.C. Strain, O. Farkas, D.K. Malick, A.D. Rabuck, K. Raghavachari, J.B. Foresman, J.V. Ortiz, Q. Cui, A.G. Baboul, S. Clifford, J. Cioslowski, B.B. Stefanov, G. Liu, A. Liashenko, P. Piskorz, I. Komaromi, R.L. Martin, D.J. Fox, T. Keith, M.A. Al-Laham, C.Y. Peng, A. Nanayakkara, M. Challacombe, P. M. W. Gill, B. Johnson, W. Chen, M. W. Wong, C. Gonzalez, Gaussian 09. Revision A.1 (Gaussian, Inc, Wallingford, CT, 2009)

    Google Scholar 

  20. W.C. Zeng, W.C. Zhang, W.H. Zhang, Q. He, B. Shi, Food Chem. Toxicol. 58, 311–317 (2013)

    Article  CAS  PubMed  Google Scholar 

  21. R.M. Lamuela-Raventós, V.L. Singleton, R. Orthofer, Methods Enzymol. 299, 152–178 (1999)

    Article  Google Scholar 

  22. M. Paczkowska, K. Lewandowska, W. Bednarski, M. Mizera, A. Podborska, A. Krause, J.C. Piontek, Spectrochim. Acta 140, 132–139 (2015)

    Article  CAS  Google Scholar 

  23. D. Jeevitha, K. Sadasivam, R. Praveena, R. Jayaprakasam, J. Mol. Struct. 1120, 15–24 (2016)

    Article  CAS  Google Scholar 

  24. A.M. Mendoza-Wilson, D. Glossman-Mitnik, J. Mol. Struct. THEOCHEM 761, 97–106 (2006)

    Article  CAS  Google Scholar 

  25. S. Valcic, J.A. Burr, B.N. Timmermann, D.C. Liebler, Chem. Res. Toxicol. 13, 801–810 (2000)

    Article  CAS  PubMed  Google Scholar 

  26. N. Russo, Food Chem. 125, 288–306 (2011)

    Article  CAS  Google Scholar 

  27. M. Leopoldini, N. Russo, M. Toscano, Food Chem. 125, 288–306 (2010)

    Article  CAS  Google Scholar 

  28. Q. Guo, B. Zhao, S. Shen, J. Hou, J. Hu, W. Xin, Biochim. Biophys. Acta 1427, 13 (1999)

    Article  CAS  PubMed  Google Scholar 

  29. A.M. Mendoza-Wilson, S.I. Castro-Arredondo, R. R. Balandrán-Quintana. Food Chem. 161, 155–161 (2014)

    Article  CAS  PubMed  Google Scholar 

  30. A.M. Mendoza-Wilson, M.E. Armenta-Vázquez, S.I. Castro-Arredondo, A. Espinosa-Plascencia, H. González-Ríos, A. González-León, R.R. Balandrán-Quintana, J. Mol. Struct. 1035, 61–68 (2013)

    Article  CAS  Google Scholar 

  31. M. Leopoldini, F. Rondinelli, N. Russo, M. Toscano, J. Agric. Food Chem. 58, 8862 (2010)

    Article  CAS  PubMed  Google Scholar 

  32. B. Zou, X. Dong, Z. Ge, Z. Xu, J. Du, C. Li, Eur. Food Res. Technol. 239, 385–391 (2014)

    Article  CAS  Google Scholar 

  33. K.R. Martin, C.G. Krueger, G. Rodriquez, M. Dreher, J.D. Reed, J. Sci. Food Agric. 89, 157–162 (2009)

    Article  CAS  Google Scholar 

  34. R.P. Feliciano, M.P. Shea, D. Shanmuganayagam, C.G. Krueger, A.B. Howell, J.D. Reed, J. Agric. Food Chem. 60, 4578 (2012)

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to Prof. Bi Shi, College of Light Industry, Textile and Food Engineering, for his helpful comments and suggestions. This work was financially supported by the National Natural Science Foundation of China (Grant No. 31801548) and Sichuan Science and Technology Program (Grant Nos. 2018HH0134, 2018GZ0003).

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Authors and Affiliations

  1. College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Mai-Rui Gao, Qian-Da Xu, Qiang He & Wei-Cai Zeng

  2. College of Life Sciences, Sichuan University, Chengdu, 610064, People’s Republic of China

    Qun Sun

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  1. Mai-Rui Gao
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  2. Qian-Da Xu
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  5. Wei-Cai Zeng
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Correspondence to Wei-Cai Zeng.

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Gao, MR., Xu, QD., He, Q. et al. A theoretical and experimental study: the influence of different standards on the determination of total phenol content in the Folin–Ciocalteu assay. Food Measure 13, 1349–1356 (2019). https://doi.org/10.1007/s11694-019-00050-6

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  • DOI: https://doi.org/10.1007/s11694-019-00050-6

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