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Cytotoxic and genotoxic activity of protein isolate of ayocote beans and anticancer activity of their protein fractions

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Abstract

We evaluated the cytotoxic and genotoxic effects of protein isolates of ayocote beans on adult male CD1+ mice and the anticancer activity of their protein fractions using SiHa Cells. The mice that received protein isolate showed a Polychromatic Erythrocytes (PCE) increase at 48 h; a significant PCE decrease was observed in mice administered the protein isolate-Daunorubicin (isolate-DAU) mix. A micronucleated erythrocytes (MNE) increase was observed in mice that received the mix in all times tested. All protein fractions of ayocote beans (black and purple) showed inhibition against SiHa cells proliferation at doses of 3000 or 5000 µg/mL. The maximal inhibitory concentration (IC50) of each protein fraction was reached at a concentration of 5000 µg/mL. A synergistic effect of isolate-DAU was observed in the in vivo test; the effect of the protein fractions on SiHa cells proliferation depended of the ayocote bean variety used.

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Acknowledgements

The authors would like to acknowledge the Autonomous University of Hidalgo State for technical services during this study.

Funding

This work was supported by the National Technology of Mexico [grant number 5938.16-P.A-P and grant number 6244.17-P].

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Authors and Affiliations

  1. Doctorado en Ciencias en Ingeniería Bioquímica, Tecnológico Nacional de México-Celaya, Antonio García Cubas Pte #600 esq. Av. Tecnológico, A. P. 57, C. P. 38010, Celaya, Guanajuato, México

    Gerardo Teniente-Martínez

  2. Tecnológico Nacional de México-Celaya, Antonio García Cubas Pte #600 esq. Av. Tecnológico, A. P. 57, C. P. 38010, Celaya, Guanajuato, México

    Gerardo Teniente-Martínez, Aurea Bernardino-Nicanor, Gerardo Acosta-García & Leopoldo González-Cruz

  3. Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Ex-Hacienda de la Concepción, Tilcuautla, 42080, Pachuca de Soto, Hidalgo, Mexico

    Raquel Cariño-Cortés & María del Carmen Valadez-Vega

  4. Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional del Instituto Politécnico Nacional, Unidad Michoacán, Justo Sierra Nº 28, C. P. 59510, Jiquilpan, Michoacan, México

    José Luis Montañez-Soto

Authors
  1. Gerardo Teniente-Martínez
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  2. Aurea Bernardino-Nicanor
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  3. Raquel Cariño-Cortés
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  5. José Luis Montañez-Soto
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  6. Gerardo Acosta-García
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  7. Leopoldo González-Cruz
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Correspondence to Leopoldo González-Cruz.

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Teniente-Martínez, G., Bernardino-Nicanor, A., Cariño-Cortés, R. et al. Cytotoxic and genotoxic activity of protein isolate of ayocote beans and anticancer activity of their protein fractions. Food Measure 13, 1040–1048 (2019). https://doi.org/10.1007/s11694-018-0019-7

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  • DOI: https://doi.org/10.1007/s11694-018-0019-7

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