Abstract
Salvia hispanica seeds were defatted by compression and this led to an increase in their fiber and protein contents. Consumption of this fiber improves bowel function and reduces blood glucose and cholesterol levels. Given its amino acids composition, S. hispanica deffated flour can have an antioxidant effect, protect the body from free radicals, and prevent inflammatory diseases. For this study, S. hispanica seeds were pressed with 22.07% of fat, 12.62% of protein, and 36.46% of fiber (d.b.). A protein concentrate was obtained from defatted flour by alkaline solubilization and acid precipitation allowing fiber separation. The concentrate had 77.26% of protein, the isolated fiber had 72.54% of protein. The concentrate was hydrolyzed with Alcalase–Flavourzyme for up to 240 min. The obtained hydrolyzates had equal degrees of hydrolysis (p < 0.05) and molecular weight of 21.99 and 34.16 kDa, corresponding to 11S globulin fractions. The antioxidant activity was measured by β-carotene discoloration, iron reducing antioxidant power and chelation (iron and copper) in hydrolyzates. The degree of hydrolysis and the first three antioxidant analyses showed comparable values (83%). Copper chelation decreased with time (values of 54–38%).
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References
R. Ayerza, W. Coates, M. Lauria, Chia seed (Salvia hispanica L.) as an omega-3 fatty acid source for broilers: influence on fatty acid composition, cholesterol and fat content of white and dark meats, growth performance, and sensory characteristics. Poult. Sci. 81(6), 826–837 (2002)
V. Ixtaina, S. Nolasco, M. Tomas, Physical properties of chia (Salvia hispanica L.) seeds. Ind. Crops Prod. 28, 286–293 (2008)
SAGARPA (Servicio de Información Agroalimentaria y Pesquera, 2016), http://www.siap.gob.mx/index.php?option=com_wrapper&view=wrapper&Itemid=350. Accessed 14 March 2016
E. Reyes, A. Tecante, M. Valdivia, Dietary fibre content and antioxidant activity of phenolic compounds present in Mexican chia (Salvia hispanica L.) seeds. Food. Chem. 107, 656–663 (2008)
G. Chabanon, I. Chevalot, X. Framboisier, S. Chenu, I. Marc, Hydrolysis of rapeseed protein isolates: kinetics, characterization and functional properties of hydrolyzates. Process Biochem. 42(10), 1419–1428 (2007)
W. Coates, R. Ayerza, Commercial production of Chia in Northwestern Argentina. J. Am. Oil Chem. Soc. 75, 1417–1420 (1998)
N. Mohd Ali, S. Yeap, W. Ho, B. Beh, S. Tan, S. Tan, The promising future of chia, Salvia hispanica L. BioMed Res. Int. 2012 (2012)
R. Craig, J. Sons, Application for approval of whole Chia (Salvia hispánica. L) seed and ground whole chia as novel food ingredients. Advisory committee for novel foods and process. (Company David Armstrong, Ireland, 2004) pp. 1–29
J. Vázquez, J. Rosado, L. Chel, D. Betancur, Dry processing of chía (Salvia hispanica L.) flour: chemical characterization of fiber and protein. CYTA J. Food 8(2), 117–127 (2010)
K. Saito, D. Hao, T. Ogawa, K. Muramoto, E. Hatakeyama, T. Yasuhara, K. Nokihara, Antioxidative properties of tripeptide libraries prepared by the combinatorial chemistry. J. Agric. Food Chem. 51, 3668–3674 (2003)
W. Pryor, D. Church, Aldehides, hydrogen peroxide, and organic radical as mediators of oxygen toxicity. Free Rad. Biol. Chem. 11, 41–46 (1991)
L. Chel, V. Pérez, D. Betancur, G. Dávila., Functional properties of flours and protein isolates from Phaseolus lunatus and Canavalia ensiformis seeds. J. Agric. Food. Chem. 50, 584–591, (2002)
C. Mazza, H. Boccalandro, C. Giordano, D. Battista, A. Scopel, C. Ballaré, Functional significance and induction by solar radiation of ultraviolet absorbing sunscreen on field-grown soybean crops. Plant Physiol. 122, 117–125 (2000)
M. Alaiz, J. Navarro, J. Girón, E. Vioque, Amino acid analysis by high-performance liquid chromatography after derivatization with diethyl ethoxymethylenemalonate. J. Chromatogr. 591, 181–186 (1992)
J. Hamada, Characterization and functional properties of rice bran proteins modified by commercial exoproteases and endoproteases. J. Food Sci. 65(2), 305–310 (2000)
M. Yust, J. Pedroche, J. Girón, M. Alaiz, F. Millán, J. Vioque, Production of ACE inhibitory peptides by digestion of chickpea legumin with Alcalase. Food. Chem. 81, 363–369 (2003)
P. Nielsen, D. Petersen, C. Damdmann, Improved method for determining food protein degree of hydrolysis. J. Food Sci. 66, 642–646 (2001)
C. Megías, M. Yust, J. Pedroche, H. Lquari, J. Girón, M. Alaiz, F. Millán, J. Vioque, Purification of an ACE inhibitory peptide after hydrolysis of sunflower protein isolates. J. Agric. Food. Chem. 52, 1928–1932 (2004)
E. Pastor, R. Juan, J. Pastor, M. Alaiz, J. Vioque, Antioxidant activity of seed polyphenols in fifteen wild Lathyrus species from South Spain. LWT Food Sci. Technol. 42, 705–709 (2009)
I. Benzie, J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239, 70–76 (1996)
P. Carter, Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Analyt. Biochem. 40(2), 450–458 (1971)
A. Saiga, S. Tanabe, T. Nidhimura, Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. J. Agric. Food. Chem. 51(12), 3361–3667 (2003)
H. Schägger, G. von Jagow, Tricine–sodium dodecil sulfate–poliacrylamide gel electrophoresis for the separation of protein in the range from 1 to 100 KDa. Anal. Biochem. 166(2), 368–379 (1987)
D. Montgomery, Diseño y análisis de experimentos, 2nd edn. (Limusa Wiley, Mexico, 2005), pp. 100–102
P.G. Peiretti, F. Gai, Fatty acid and nutritive quality of chia (Salvia hispanica L.) seeds and plant during growth. Anim. Feed Sci. Technol. 148(2), 267–275 (2009)
R. Ayerza, W. Coates, The omega-3 enriched eggs: the influence of dietary linolenic fatty acid source combination on egg production and composition. Can. J. Anim. Sci. 81, 355–362 (2001)
M. Capitani, V. Spotorno, S. Nolasco, M. Tomás, Physicochemical and functional characterization of by-products from chia (Salvia hispanica L.) seeds of Argentina. LWT Food Sci. Technol. 45(1), 94–102 (2012)
J. Pedroche, M. Yust, J. Girón, M. Alaiz, F. Millán, J. Vioque, Utilization of chickpea protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity. J. Sci. Food Agric. 960–964 (2002)
M. Perez, M. Serra, M. Del Rio, Color change of fresh-cut apples coated with whey protein concentrate-based edible coatings. Postharvest Biol. Technol. 39(1), 84–92 (2006)
H. Korhonen, A. Pihlanto, Bioactive peptides: Production and functionality. Int. Dairy J. 16, 945–960 (2006)
A. Papadopoulou, R. Frazier, Characterization of protein-polyphenol interactions. Trends Food Sci. Technol. 15(3–4), 186–190 (2004)
L. Xu, L. Diosady, Interactions between canola proteins and phenolic compounds in aqueous media. Food Res. Int. 33(9), 725–731 (2000)
FAO, Energy and Protein Requirements. (FAO/WHO/UNU, Geneva, 1985)
G. Schaafsma, The protein digestibility–corrected amino acid score. J. Nutr. 130(7), 1865S–1867S (2000)
C. Megías, J. Pedroche, M. Yust, M. Alaíz, J. Girón, F. Millán, J. Vioque, Affinity purification of copper-chelating peptides fron sunflower protein hydrolyzates. J. Agric. Food Chem. 55(16), 6509–6514 (2007)
J. Carrasco, A. Hernández, C. Jiménez, C. Jacinto, M. Alaiz, J. Girón, G. Dávila, Antioxidant and metal chelating activities of peptide fractions from phaseolin and bean protein hydrolyzates. Food Chem. 135(3), 1789–1795 (2012)
B. Wroblewska, M. Karamac, R. Amarowicz, A. Szymkiewicz, A. Troszynka, E. Kubicka, Inmunoreactive properties of peptide fractions of cow whey milk proteins alter enzymatic hidrolysis. Int. J. Food Sci. Technol. 39, 839–850 (2004)
A. Dávalos, M. Miguel, B. Bartolomé, R. López-Fandiño, Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolisis. J. Food Protect. 67, 1939–1944 (2004)
R. Eisenthal, M. Danson, (eds.), Enzyme Assays, 2nd edn. Practical Approach Series; 257 (Oxford University Press, 2002), pp. 20–22
I. Salazar, C. Segura, L. Chel, D. Betancur, in Scientific, Health and Social Aspects of the Food Industry, ed. by B. Valdez. Antihypertensive and antioxidant effects of functional foods containing chia protein hydrolyzates, (InTech, Rijeka, 2012) ISBN 978-953-307-916-5, pp. 381–398
L. Chel, M. Domínguez, A. Martínez, G. Dávila, D. Betancur, Lima bean (Phaseolus lunatus) protein hydrolyzates with ACE-I inhibitory activity. Food Nutr. Sci. 3(4), 511–521 (2012)
D. Marrufo, M. Segura, L. Chel, D. Betancur, Defatted Jatropha curcas flour and protein isolate as materials for protein hydrolyzates with biological activity. Food. Chem. 138(1), 77–83 (2013)
M. Sandoval, O. Paredes, Isolation and characterization of proteins from chia seeds (Salvia hispanica L.). J. Agric. Food Chem. 61(1), 193–201 (2012)
D. Doucet, D.E. Otter, S.F. Gauthier, E.A. Foegeding, Enzyme-induced gelation of extensively hydrolyzed whey proteins by Alcalase: peptide identification and determination of enzyme specificity. J. Agric. Food. Chem. 51(21), 6300–6308 (2003)
X. Peng, B. Kong, X. Xia, Q. Liu, Reducing and radical-scavenging activities of whey protein hydrolyzates prepared with Alcalase. Int. Diary J. 20(5), 360–365 (2010)
L. Zhu, J. Chen, X. Tang, Y. Xiong, Reducing, radical scavenging, and chelation properties of in vitro digests of Alcalase-treated zein hydrolyzate. J. Agric. Food Chem. 56, 2714–2721 (2008)
T. Chuang-He, W. Xiang-Sheng, Y. Xiao-Quan, Enzymatic hydrolysis of hemp (Cannabis sativa L.) protein isolate by various proteases and antioxidant properties of the resulting hydrolyzates. Food Chem. 114(4), 1484–1490 (2009)
K. Zhu, H. Zhou, H. Quian, Antioxidant and free radical-scavenging activities of wheat germ proteins hydrolyzates (WGPH) prepared with Alcalase. Process Biochem 41(6), 1296–1302 (2006)
B. Kong, Y. Xiong, Antioxidant activity of zein hydrolyzates in a liposome system and the possible mode of action. J. Agric. Food Chem. 54, 6059–6068 (2006)
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Chim-Chi, Y., Gallegos-Tintoré, S., Jiménez-Martínez, C. et al. Antioxidant capacity of Mexican chia (Salvia hispanica L.) protein hydrolyzates. Food Measure 12, 323–331 (2018). https://doi.org/10.1007/s11694-017-9644-9
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DOI: https://doi.org/10.1007/s11694-017-9644-9