Skip to main content

Advertisement

Log in

Characterization of nutritionally important phytoconstituents in minimally processed ready-to-eat baby-leaf vegetables using HPLC–DAD and GC–MS

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

Ready-to-eat baby-leaf vegetable market has been rapidly growing and offering to consumers convenient and appealing products, rich in health beneficial bioactive compounds. In the present study, the composition of carotenoids, tocopherols, and fatty acids were analyzed in seven baby-leaf vegetables using HPLC–DAD and GC–MS. Among the vegetables, the maximum amount (μg/g FW) of All-E-violaxanthin (42.77), 9′-Z-neoxanthin (22.13), All-E-lutein (69.67), All-E-β-carotene (60.18), total carotenoids (195.21), γ-tocopherol (19.68) and total tocopherol (47.68) were found in Batavian lettuce (Lactuca sativa L. var. Acephala). In all the studied baby-leaf vegetables, α-linolenic acid (ALA, C18:3) was found in highest quantity (44.73–54.39 %) followed by palmitic acid (C16:0) (13.02–19.49 %), and linoleic acid (C18:2) (8.25–21.54 %). Significantly high amount polyunsaturated fatty acids (PUFA) were recorded in Batavian lettuce (74.33 %) and red Romaine (72.72 %), compared to other studied vegetables. In view of health benefits, baby-leaf vegetables contain a low amount of saturated fatty acids and high-mono and PUFA, which can enhance the health benefits of these vegetables. Carotenoids in most of these studied baby-leaf vegetables can be classified as very high. Knowledge of carotenoid, tocopherols and fatty acids composition in different baby-leaf vegetables will be useful to nutritional experts for selection of nutrient-dense plants for food fortification and proper diet recommendation. To our knowledge, this is the first report on fatty acids composition from baby-leaf vegetables.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. H. Boeing, A. Bechthold, A. Bub, S. Ellinger, D. Haller, A. Kroke, E. Leschik-Bonnet, M.J. Müller, H. Oberritter, M. Schulze, P. Stehle, B. Watzl, Eur. J. Nutr. 51, 637 (2012)

    Article  CAS  Google Scholar 

  2. D. Granato, M.R. Alezandro, F. Nazzaro, Food Res. Int. 77, 73 (2015)

    Article  Google Scholar 

  3. A. Martínez-Sánchez, M.C. Luna, M.V. Selma, J.A. Tudela, J. Abad, M.I. Gil, Postharvest Biol. Technol. 63, 1 (2012)

    Article  Google Scholar 

  4. J. Santos, M. Herrero, J.A. Mendiola, M.T. Oliva-Teles, E. Ibáñez, C. Delerue-Matos, M.B.P.P. Oliveira, Food Res. Int. 58, 105 (2014)

    Article  CAS  Google Scholar 

  5. A. López, G.-A. Javier, J. Fenoll, P. Hellín, P. Flores, J. Food Compos. Anal. 33, 39 (2014)

    Article  Google Scholar 

  6. J. Santos, M.T. Oliva-Teles, C. Delerue-Matos, M.B.P.P. Oliveira, Food Chem. 151, 311 (2014)

    Article  CAS  Google Scholar 

  7. J. Ju, S.C. Picinich, Z. Yang, Y. Zhao, N. Suh, A.-N. Kong, C.S. Yang, Carcinogenesis 31, 533 (2010)

    Article  CAS  Google Scholar 

  8. R.K. Saini, S.H. Nile, S.W. Park, Food Res. Int. 76, 735 (2015)

    Article  CAS  Google Scholar 

  9. E. Mocchegiani, L. Costarelli, R. Giacconi, M. Malavolta, A. Basso, F. Piacenza, R. Ostan, E. Cevenini, E.S. Gonos, C. Franceschi et al., Ageing Res. Rev. 14, 81 (2014)

    Article  CAS  Google Scholar 

  10. A. Kamal-Eldin, E. Budilarto, in Handbook of Antioxidants for Food Preservation, ed. by F. Shahidi (Woodhead Publishing Ltd, 2015), p. 141

  11. J.J. Thiele, S.N. Hsieh, S. Ekanayake-Mudiyanselage, Dermatol. Surg. 31, 805 (2005)

    Article  CAS  Google Scholar 

  12. R.K. Saini, N.P. Shetty, P. Giridhar, J. Am. Oil Chem. Soc. 91, 1029 (2014)

    Article  CAS  Google Scholar 

  13. A.P. Simopoulos, Am. J. Clin. Nutr. 70, 560s (1999)

    CAS  Google Scholar 

  14. S.Å. Bergquist, U.E. Gertsson, M.E. Olsson, J. Sci. Food Agric. 86, 346 (2006)

    Article  CAS  Google Scholar 

  15. Z. Xiao, G.E. Lester, Y. Luo, Q. Wang, J. Agric. Food Chem. 60, 7644 (2012)

    Article  CAS  Google Scholar 

  16. J.G. Kim, Acta Hortic. 746, 33–38 (2007). doi:10.17660/ActaHortic.2007.746.2

    Article  Google Scholar 

  17. F. Ferioli, E. Giambanelli, L.F. D’Antuono, H.S. Costa, T.G. Albuquerque, A.S. Silva, O. Hayran, B. Koçaoglu, J. Sci. Food Agric. 93, 3478 (2013)

    Article  CAS  Google Scholar 

  18. D.M. Jiménez-Aguilar, M.A. Grusak, Plant Foods Hum. Nutr. 70(4), 357–364 (2015)

    Article  Google Scholar 

  19. R.K. Saini, N.P. Shetty, P. Giridhar, Eur. Food Res. Technol. 238, 971 (2014)

    Article  CAS  Google Scholar 

  20. I. Sivanesan, R.K. Saini, D.H. Kim, Ind. Crops Prod. 83, 31 (2016)

    Article  CAS  Google Scholar 

  21. R.K. Saini, N.P. Shetty, P. Giridhar, G.A. Ravishankar, 3 Biotech 2, 187 (2012)

    Article  Google Scholar 

  22. Food Drug Adm, Cent. Drug Eval. Res. 1, 124 (2001)

    Google Scholar 

  23. R. Cruz, S. Casal, Food Chem. 141, 1175 (2013)

    Article  CAS  Google Scholar 

  24. D.B. Rodriguez-Amaya, A guide to carotenoid analysis in foods (ILSI PRESS, Washington, D. C., 2001)

    Google Scholar 

  25. C. Reif, E. Arrigoni, H. Schärer, L. Nyström, R.F. Hurrell, J. Food Compos. Anal. 29, 64 (2013)

    Article  CAS  Google Scholar 

  26. J. Santos, J.A. Mendiola, M.B.P.P. Oliveira, E. Ibáñez, M. Herrero, J. Chromatogr. A 1261, 179 (2012)

    Article  CAS  Google Scholar 

  27. J.G. Provesi, C.O. Dias, E.R. Amante, Food Chem. 128, 195 (2011)

    Article  CAS  Google Scholar 

  28. B. Mou, J. Am. Soc. Hortic. Sci. 130, 870 (2005)

    CAS  Google Scholar 

  29. R. Lakshminarayana, M. Raju, T.P. Krishnakantha, V. Baskaran, J. Agric. Food Chem. 53, 2838 (2005)

    Article  CAS  Google Scholar 

  30. G. Samuolienė, A. Brazaitytė, R. Sirtautas, A. Viršilė, J. Sakalauskaitė, S. Sakalauskienė, P. Duchovskis, J. Sci. Food Agric. 93, 3286 (2013)

    Article  Google Scholar 

  31. G. Britton, F. Khachik, in Analysis of carotenoids in nutritional studies, ed. by G. Britton, S. Liaaen-Jensen, H. Pfander (Birkhäuser, Basel, 2009), p. 45

    Google Scholar 

  32. G. Barceló-Coblijn, E.J. Murphy, Prog. Lipid Res. 48, 355 (2009)

    Article  Google Scholar 

  33. J. Aranceta, C. Pérez-Rodrigo, Br. J. Nutr. 107, S8 (2012)

    Article  CAS  Google Scholar 

  34. R. Vidrih, S. Filip, J. Hribar, Czech J. Food Sci. 27, S125 (2009)

    CAS  Google Scholar 

  35. A. Topolewska, K. Czarnowska, Ł.P. Haliński, P. Stepnowski, J. Chromatogr. B 990, 150 (2015)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This paper was supported by KU research professor program of Konkuk University, Seoul, Republic of Korea. Also, financial support from export promotion technology development program, the ministry of agriculture, food and rural affairs, Republic of Korea is highly acknowledged.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Ramesh Kumar Saini or Young-Soo Keum.

Ethics declarations

Conflict of interest

The authors have declared that there is no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 1287 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saini, R.K., Shang, X.M., Ko, E.Y. et al. Characterization of nutritionally important phytoconstituents in minimally processed ready-to-eat baby-leaf vegetables using HPLC–DAD and GC–MS. Food Measure 10, 341–349 (2016). https://doi.org/10.1007/s11694-016-9312-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-016-9312-5

Keywords

Navigation