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Trichinella spiralis (Owen, 1835) Induces Increased Dystrophin Expression in Invaded Cross-striated Muscle

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Abstract

Purpose

Dystrophin and the dystrophin glycoprotein complex serve as a cytoskeletal integrator, critical for muscle membrane stability. The aim of the present study was to clarify the expression of dystrophin protein and mRNA in the skeletal muscle tissue during the muscle phase of trichinellosis in mice.

Methods

Muscle tissue was collected from mice experimentally infected with Trichinella spiralis at days 0, 14 and 40 after infection. The expression of dystrophin in the muscle tissue was investigated by immunohistochemistry with antibodies against three different domains of the protein, and the expression levels of Dys mRNA by real-time PCR.

Results

The presence of dystrophin protein was increased in the de-differentiating cytoplasm at the early stage of muscle infection and was persisting also in the mature Nurse cell harbouring the parasite. It was accompanied by significantly elevated expression of Dys mRNA at days 14 and 40 after infection.

Conclusion

Our findings indicate that dystrophin plays a role in regeneration of the muscle and in the Nurse cell formation and stability for security of the parasite survival.

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Data availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was financially supported by the Bulgarian National Science Fund under Grant DN01/16.

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Authors and Affiliations

  1. Institute of Experimental Morphology, Pathology and Anthropology with Museum—Bulgarian Academy of Sciences, “Acad. G. Bonchev’’ Str., block 25, 1113, Sofia, Bulgaria

    Rositsa Milcheva, Katerina Todorova, Valeria Dilcheva & Svetlozara Petkova

  2. Unilabs Slovensko, s.r.o., Bratislava, Slovakia

    Iveta Mečiarová

  3. Institute of Pathological Anatomy, Faculty of Medicine, Comenius University in Bratislava, Bratislava, Slovakia

    Pavel Babál

Authors
  1. Rositsa Milcheva
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  2. Iveta Mečiarová
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  3. Katerina Todorova
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  4. Valeria Dilcheva
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  5. Svetlozara Petkova
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  6. Pavel Babál
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Contributions

PB and RM—conceptualization and design of research. RM, IM, KT, VD and SP—performance of research. PB, IM, RM, KT and VD—data analysis. RM—manuscript writing. PB—improvement and final edition of manuscript.

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Correspondence to Rositsa Milcheva.

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Milcheva, R., Mečiarová, I., Todorova, K. et al. Trichinella spiralis (Owen, 1835) Induces Increased Dystrophin Expression in Invaded Cross-striated Muscle. Acta Parasit. 68, 393–399 (2023). https://doi.org/10.1007/s11686-023-00673-2

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