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Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis

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Abstract

Introduction

Cystic Echinococcosis (CE) is a chronic parasitic disease caused by the metacestodes of Echinococcus granulosus senso lato (E. granulosus s.l.). The larval stages of this parasite, hydatid cyst, are usually diagnosed using imaging modalities and serological testing; however, several studies have recently suggested using the parasite-derived microRNAs (miRNAs) as novel diagnostic biomarkers.

Materials and Methods

The present study included 31 CE patients who were older than 5 years and were admitted to the hospitals of Ahvaz, Iran for hydatid cyst removal surgery during 2019–2021. The egr-miR-125-5p and egr-miR-2a-3p levels were evaluated in the sera of the CE patients before and 6 months after the surgery using Quantitative Real-Time PCR (qRT-PCR), and the results were compared with the serum samples from 15 healthy volunteers. Then, the intergroup comparisons were performed using the t test.

Results

The patients’ age range was 6–72 years, with a mean age of 34.6 years. Moreover, based on the classification by the WHO-IWGE, one patient (3.2%) had CE1, 14 patients (45.2%) had CE2, 5 patients (16.1%) had CE3, 2 patients (6.5%) had CE4, and 9 patients (29%) had CE5. Also, 21 patients (67.74%) had a positive antigen test using the ELISA method, while 10 patients (32.26%) had a negative ELISA. The pre-operative expression level of egr-miR-2a-3p was 10.36 folds higher compared to 6 months after the surgery, with an AUC value of 0.8176. However, the expression levels of egr-miR-125-5p did not change significantly 6 months after the surgery compared to pre-operative levels.

Conclusions

According to the present study results, the serum levels of egr-miR-2a-3p can be a promising non-invasive biomarker for diagnosing CE and monitoring its potential recurrence after cystectomy.

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Data availability statement

All data generated or analyzed during the current study are included in the paper.

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Acknowledgements

The authors would like to thank all the patients participating in the present study and the healthcare providers of the hospitals in Ahvaz for their contributions in the process of sampling. Moreover, the present study was a part of a Ph.D. thesis by M. Fasihi Karami.

Funding

The Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, provided financial support for this project under the grant number CMRC-0022.

Author information

Authors and Affiliations

  1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Maryam Fasihi Karami, Molouk Beiromvand & Dian Dayer

  2. Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Molouk Beiromvand & Mahmoud Rahdar

  3. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Abdollah Rafiei

  4. Department of Surgery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Amin Bahreini & Ali Asghar Dastyar

Authors
  1. Maryam Fasihi Karami
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Contributions

The present study was designed by MB and AR. All examinations performed by MFK. Data analysis was performed by MB, MFK, DD and MR. AB and AD were the surgeons who operated on the patients. MB, AR, and MFK wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Molouk Beiromvand.

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Karami, M.F., Beiromvand, M., Rafiei, A. et al. Serum Level of egr-miR-2a-3p as a Potential Diagnostic Biomarker for Cystic Echinococcosis. Acta Parasit. 68, 114–121 (2023). https://doi.org/10.1007/s11686-022-00641-2

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