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Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

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Abstract

The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IVembryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.

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Acknowledgements

This study was supported by the Chinese Society of Reproductive Medicine (No. 16020520668).

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Authors and Affiliations

  1. Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu & Lei Jin

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  1. Bo Huang
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  2. Zhou Li
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Correspondence to Lixia Zhu or Lei Jin.

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Huang, B., Li, Z., Ren, X. et al. Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality. Front. Med. 11, 247–252 (2017). https://doi.org/10.1007/s11684-017-0519-6

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  • DOI: https://doi.org/10.1007/s11684-017-0519-6

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