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Lipophilic extractives of the inner and outer barks from six different Pinus species grown in Indonesia

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Abstract

The chemical compositions of the dichloromethane extracts of inner and outer barks from six Pinus species (P. elliotii, P. oocarpa, P. caribeae, P. merkusii, P. montezumae, and P. insularis) grown in Indonesia were investigated by GC and GC–MS. Generally, the amounts of extractive contents were higher in the inner bark than in the outer bark except for P. merksuii. Fatty acids, monoterpenes, sesquiterpenes, resin acids, triterpenoids, and steroids were detected and quantified. Inner and outer barks differed not only in content of these compounds but also in their composition. Fatty acids and alcohols were the major classes of lipophilic compounds in the outer bark of P. caribeae, P. insularis, and P. montezumae. Steroids and triterpenoids were the dominant compounds identified in the inner bark of P. elliotii, P. insularis, and P. merkusii. Resin acids were the most abundant group in the inner bark of P. oocarpa whereas monoterpenes and sesquiterpenes were recorded in minor quantities in both bark layers of all species.

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References

  • Aspé E, Fernández K (2011) Comparison of phenolic extracts obtained of Pinus radiata bark from pulp and paper industry and sawmill industry. Maderas Cienc Tecnol 13(3):243–252

    Article  CAS  Google Scholar 

  • Back EL, Allen LH (eds) (2000) Pitch control, wood resin and deresination. Tappi Press, Atlanta

    Google Scholar 

  • Baeza J, Juanita F (2001) Chemical characterization of wood. In: Hon DNS, Shiraishi N (eds) Wood and cellulosic chemistry, 2nd edn. Marcel Dekker, New York, pp 275–384

    Google Scholar 

  • Berrocal A, Baeza J, Rodriguez J, Espinosa M, Freer J (2004) Effect of tree age on variation of Pinus radiate D. on chemical composition. J Chil Chem Soc 49:252–256

    Article  Google Scholar 

  • Bikovens O, Roze L, Pranovich A, Reunanen M, Telysheva G (2013) Chemical composition of lipophilic extractives from grey alder (Alnus incana). Bioreources 8(1):350–357

    Google Scholar 

  • Chowdhury JU, Bhuiyan MDNI, Nandi NC (2008) Essential oil constituents of needles, dry needles, inflorescences, and resins of P.caribaea Morelet growing in Bangladesh. Bangladesh J Bot 37(2):211–212

    Google Scholar 

  • Coppen JJW, James DJ, Robinson JM, Subansenee W (1998) Variability in xylem resin composition amongst natural population of Thai and Filipino Pinus merkusii de Vriese. Flavour Fragr J 13:33–39

    Article  CAS  Google Scholar 

  • Evdokimova OV, Neneleva EV, Tarrab I, Glazvoka IY (2013) Comparison of lipophilic substances of the bark of Chinese (Cinnamomun cassia (L.) C. Presl.) and Ceylon Cinnamon (Cinnamomum zeylanicum Blume). World Appl Sci J 27(1):70–73

    Google Scholar 

  • Fang JM, Cheng YS (1992) Chemical constituents of some endemic conifers in Taiwan. J Chin Chem Soc 39:647–654

    Article  CAS  Google Scholar 

  • Fang JM, Tsai WY, Cheng YS (1991) Serratene triterpene from Pinus armandii bark. Phytochemistry 30(4):1333–1336

    Article  CAS  Google Scholar 

  • Fengel D, Wegener G (1989) Wood: chemistry, ultrastructure, reactions. Walter de Gruyter, Berlin

    Google Scholar 

  • Freire CSR, Silvestre AJD, Neto CP, Cavaleiro JAS (2002) Lipophilic extractives of the inner and outer barks of Eucalyptus globulus. Holzforschung 56:372–379

    CAS  Google Scholar 

  • Gershenzon J (1994) Metabolic costs of terpenoid accumulation in higher plants. J Chem Ecol 120:1281–1328

    Article  Google Scholar 

  • Gutiěrrez A, del Rio JC, Fransisco J, Gonźales-Vila Martin F (1999) Chemical composition of lipophilic extractives from Eucalyptus globulus Labill. wood. Holzforschung 53:481–486

    Article  Google Scholar 

  • Gutiěrrez A, Romero J, del Rio JC (2001) Lipophilic extractives from Eucalyptus globulus pulp during kraft cooking followed by TFC and ECF bleaching. Holzforschung 55:260–264

    Article  Google Scholar 

  • Hafizoğlu H, Holmborn B, Reunanen M (2002) Chemical composition of lipophilic and phenolic constituents of bark from Pinus nigra, Abies bornműlleriana, and Castanea sativa. Holzforschung 56:257–260

    Article  Google Scholar 

  • Kilulya KF, Msagati TAM, Mamba BB, Ngila JC, Bush T (2012) Determination of lipophilic extractives in ionic liquid extracts of Eucalyptus pulp by gas chromatography- mass spectrometry. Tanzan J Sci 83(3):14–26

    Google Scholar 

  • Krogell J, Holmbom B, Pranovich A, Hemming J, Willför S (2012) Extraction and chemical characterization of Norway spruce inner and outer bark. Nord Pulp Paper Res J 27:6–17

    Article  CAS  Google Scholar 

  • Ku CS, Jang JP, Mun SP (2007) Exploitation of polyphenol-rich pine barks for antioxidant activity. J Wood Sci 53:524–528

    Article  CAS  Google Scholar 

  • Martínez-Iñigo MJ, Gutiérrez A, del Río JC, Martínez MJ, Martínez AT (2001) Time course of fungal removal of lipophilic extractives from Eucalyptus globulus wood. J Biotechnol 84(2):119–126

    Article  PubMed  Google Scholar 

  • Masendra, Ashitani T, Takahashi K, Lukmandaru G (2017) Triterpenoids and steroids from the bark of Pinus merkusii (Pinaceae) (in submission)

  • Norin T, Winell B (1972) Extractives from the bark of scots pine, Pinus silvestris L. Acta Scand 26:2297–2304

    Article  CAS  Google Scholar 

  • Nunes E, Quilhό T, Pereira H (1999) Anatomy and chemical composition of P. pinea L. bark. Ann For Sci 56:479–484

    Article  Google Scholar 

  • Oliveira L, Freire CSR, Silvestre AJD, Cordeiro N, Torres IC, Evtugin D (2006) Lipophili extractives from different morphological parts of banana plant “Dwarf Cavendish”. Ind Crops Prod 23:201–211

    Article  CAS  Google Scholar 

  • Rahman MM, Mohammad S (2011) Study on fatty acid composition and physic-chemical properties of Nyctanthes arbortristis root. J Bangladesh Chem Soc 24(2):202–208

    CAS  Google Scholar 

  • Rowe JW (1964) Triterpenes of pine barks: identity of pinusenediol and serratenediol. Tetrahedron Lett 5:2347–2353

    Article  Google Scholar 

  • Sato M, Seki K, Kita K (2009) Comparative analysis of diterpene composition in the bark of the hybrid larch F1, Larix gmelinii var. japonica × L. kaempferi and their parent trees. J Wood Sci 55:32–40

    Article  CAS  Google Scholar 

  • Seki K, Orihashi K, Sato M (2012) Accumulation of constitutive diterpenoids in the rhytidome and secondary phloem of the branch bark of Larix gmelinii var. japonica. J Wood Sci 58:437–445

    Article  CAS  Google Scholar 

  • Silliman K, Parry J, Kirk LL, Prior RL (2003) Pycnogenol does not impact the antioxidant or vitamin C status of healthy young adults. J Am Diet Assoc 103:67–72

    Article  PubMed  Google Scholar 

  • Valentín L, Kluczek-Turpeinen B, Willför S, Hemming J, Hatakka A, Steffen K, Toumela M (2010) Scots pine (Pinus sylvestris) bark composition and degradation by fungi: potential substrate for bioremediation. Bioresour Technol 101:2203–2209

    Article  PubMed  CAS  Google Scholar 

  • Wijayanto A, Dumacay S, Gerardin-Charbonnier C, Sari RK, Syafii W, Gerardin P (2015) Phenolic and lipophilic extractives in Pinus merkusii Jungh. Et de Vries Knots and stemwood. Ind Crops Prod 69:466–471

    Article  CAS  Google Scholar 

  • Willför S, Hemming J, Reunanen M, Holmbom B (2003) Phenolic and lipophilic extractives in scots pine knots and stemwood. Holzforschung 57:359–372

    Google Scholar 

  • Willför S, Ali M, Karonen M, Reunanen M, Arfan M, Harlamow R (2009) Extractives in bark of different conifer species growing in Pakistan. Holzforschung 63:551–558

    Article  CAS  Google Scholar 

  • Wittstock U, Gershenzon J (2002) Constitutive plant toxins and their role in defense against herbivores and pathogens. Curr Opin Plant Biol 5:300–307

    Article  PubMed  CAS  Google Scholar 

  • Wiyono B, Tachibana S, Tinambunan D (2006) Chemical composition of Indonesian Pinus merkusii turpentine oils, gum oleoresins and rosins from Sumatra and Java. Pak J Biol Sci 9(1):7–14

    Article  CAS  Google Scholar 

  • Yamamoto H, Ookuba Y, Ikeda A, Kusano A, Tanaka K, Matsukawa S (2011) Terpenes isolated from the bark and wood of Pinus luchuensis. Bull Fac Educ Ibaraki Univ (Nat Sci) 60:91–100

    Google Scholar 

  • Yesil-Celiktas O, Ganzera M, Akgun I, Sevimli C, Kormaz KS, Bedir E (2009) Determination of polyphenolic constituents and biological activities of bark extracts from different Pinus species. J Sci Food Agric 89:1339–1345

    Article  CAS  Google Scholar 

  • Zule J, Čufar K, Tišler V (2015) Lipophilic extractives in heartwood of European Larch (Larix decidua Mill). Drvna Ind 66(4):305–315

    Article  Google Scholar 

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Acknowledgements

We thank the Centre for Forest Biotechnology and Tree Improvement Research for providing research materials.

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Correspondence to Ganis Lukmandaru.

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Project Funding: This work was supported by JASSO (Japan Student Services Organization) and DPP Grant 2016 (Faculty of Forestry, UGM).

The online version is available at http://www.springerlink.com

Corresponding editor: Yu Lei.

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Masendra, Ashitani, T., Takahashi, K. et al. Lipophilic extractives of the inner and outer barks from six different Pinus species grown in Indonesia. J. For. Res. 29, 1329–1336 (2018). https://doi.org/10.1007/s11676-017-0545-x

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  • DOI: https://doi.org/10.1007/s11676-017-0545-x

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