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BpMADS12 gene role in lignin biosynthesis of Betula platyphylla Suk by transcriptome analysis

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Abstract

MADS-box transcription factors show highly diverse regulatory functions in a wide variety of organisms. In this study, we characterized a MADS-box gene (BpMADS12) from the white birch (Betula platyphylla Suk). This gene is a member of the suppressor of overexpression of CO 1/tomato MADS 3 class of MADS-box genes. We generated lines overexpressing BpMADS12 and found that these had higher levels of lignin compared to that observed in nontransgenic lines. Transcriptome analysis revealed numerous changes in gene expression patterns. In total, 8794 differentially expressed genes were identified, including 5006 upregulated unigenes and 3788 downregulated unigenes in BpMADS-overexpression lines. Differentially expressed genes involved in the pathways for lignin and brassinosteroid biosynthesis were significantly enriched and may have contributed to phenotypic changes. The results from a quantitative RT-PCR analysis were consistent those obtained with the transcriptome analysis. Our transcriptome analysis, in combination with measurement of lignin level, indicated that BpMADS12 promotes lignin synthesis through regulation of key enzymes in response to brassinosteroid signaling. These results suggest that this MADS-box protein is crucial to all subsequent structural events and provide a good foundation for studies aiming to elucidate the developmental mechanisms underlying formation of wood.

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, China

    Huiyu Li, Yang Yang, Zijia Wang, Feifei Liu, Jing Jiang & Guifeng Liu

  2. College of Agricultural Sciences, Heilongjiang Bayi Agricultural University, Xinyang Road 2, Daqing, 163319, China

    Xiaohong Guo

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  1. Huiyu Li
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  2. Yang Yang
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  3. Zijia Wang
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  4. Xiaohong Guo
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  5. Feifei Liu
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  6. Jing Jiang
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  7. Guifeng Liu
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Correspondence to Guifeng Liu.

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Project funding: This work was financially supported by the National Science and Technology Program of China during the 12th Five-Year Plan Period (No. 2013AA102704) and the National Natural Science Foundation of China (NO: 31200510).

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Corresponding editor: Yu Lei

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Li, H., Yang, Y., Wang, Z. et al. BpMADS12 gene role in lignin biosynthesis of Betula platyphylla Suk by transcriptome analysis. J. For. Res. 27, 1111–1120 (2016). https://doi.org/10.1007/s11676-016-0229-y

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