Abstract
Cotoneaster melanocarpus Fisch. ex A.Blytt (Rosaceae) is a deciduous shrub that is difficult to propagate through traditional methods. Thus, in the present study, in vitro regeneration utilizing nodal explants was attempted for the first time. Murashige and Skoog (MS) and Quoirin and Lepoivre (QL) medium supplemented with 0.2 or 0.5 mg L−1 6-benzyladenine (BA), 0.01 mg L−1 indole-3-butyric acid (IBA), and 0.5 mg L−1 gibberellic acid (GA3) were utilized for shoot culture establishment. Optimum 8.83 ± 0.66 shoots with 0.87 ± 0.10 cm length and 4.27 ± 0.37 leaves per explant were recorded after 40 d in MS medium fortified with 0.2 mg L−1 BA, 0.01 mg L−1 IBA, and 0.5 mg L−1 GA3. These shoots were then rooted in ½MS medium augmented with 0.5 mg L−1 IBA which formed 4.03 ± 0.29 roots per shoot (100% response) with 3.64 ± 0.16 cm length. The plantlets were then transferred to soil:vermiculite (3:1 w/w) in the light room for 35 d. Later on, they were successfully hardened and acclimatized in open ground with 80.9% survival rate after 3 mo.
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This research is funded by the Ministry of Ecology, Geology and Natural Resources of the Republic of Kazakhstan (No. BR10263776).
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Kirillov, V., Pathak, A., Patel, S.R. et al. Micropropagation of Cotoneaster melanocarpus Fisch. ex A.Blytt: an economically important ornamental plant. In Vitro Cell.Dev.Biol.-Plant 59, 147–153 (2023). https://doi.org/10.1007/s11627-023-10332-w
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DOI: https://doi.org/10.1007/s11627-023-10332-w