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Optimization of culture conditions for the production of polysaccharides and kinsenoside from the rhizome cultures of Anoectochilus roxburghii (Wall.) Lindl.

  • Bioactive/Medicinal Compounds
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Abstract

The present study designed two sets of experiments by using the uniform design method and investigated the effects of medium components on the accumulation of bioactive compounds (polysaccharide and kinsenoside) in rhizomes, in order to select a suitable culture medium for the rhizome suspension culture of Anoectochilus roxburghii (Wall.) Lindl. Among the combinations of Murashige and Skoog (MS) medium strengths and plant growth regulator (benzylaminopurine, BA; kinetin, KT; and α-naphthaleneacetic acid, NAA) concentrations, and the combinations of nitrogen, phosphorus, and sucrose concentrations, the maximum yield of polysaccharides and kinsenoside was achieved with 0.75 × MS + 2.0 mg L−1 BA + 0.2 mg L−1 KT + 0.5 mg L−1 NAA and 45 mM nitrogen + 0.93 mM phosphorus + 35 g L−1 sucrose, respectively. Therefore, the optimal rhizome suspension culture medium was 0.75 × MS medium supplemented with 2.0 mg L−1 BA, 0.2 mg L−1 KT, 0.5 mg L−1 NAA, and 35 g L−1 sucrose. Yeast extract (YE) enhanced bioactive compound accumulation in rhizomes. The polysaccharide and kinsenoside production was significantly improved when 75 mg L−1 YE was added to the culture medium after 30 d of rhizome suspension culture; 8.3 g L−1 of polysaccharide and 6.1 g L−1 of kinsenoside were obtained after 4 d of YE treatment. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of YE-treated rhizomes was higher than that of YE-untreated rhizomes, demonstrating enhanced antioxidant activity of the treated bioreactor-cultured rhizomes.

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Funding

Project 31660080 was supported by the National Natural Science Foundation of China and project no. 23 of 2017 was supported by Youth Foundation of Yanbian University.

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Author notes

  1. Mei Yu Jin and Li Qiu Zhang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Natural Resource of Changbai Mountain and Functional Molecules, Yanbian University, Ministry of Education, Park Road 977, Yanji, Jilin, 133002, China

    Mei Yu Jin, Xuan Chun Piao, Ri Gao & Mei Lan Lian

  2. Tonghua Normal University, Tonghua, Jilin, 134002, China

    Li Qiu Zhang

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  1. Mei Yu Jin
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  2. Li Qiu Zhang
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  3. Xuan Chun Piao
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  4. Ri Gao
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Correspondence to Mei Lan Lian.

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Editor: Randall P. Niedz

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Jin, M.Y., Zhang, L.Q., Piao, X.C. et al. Optimization of culture conditions for the production of polysaccharides and kinsenoside from the rhizome cultures of Anoectochilus roxburghii (Wall.) Lindl.. In Vitro Cell.Dev.Biol.-Plant 54, 25–35 (2018). https://doi.org/10.1007/s11627-017-9883-9

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  • DOI: https://doi.org/10.1007/s11627-017-9883-9

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