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Effects of plant growth regulators and explant types on in vitro direct plant regeneration of Agastache foeniculum, an important medicinal plant

  • Micropropagation
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Abstract

This study presents in vitro direct regeneration for Agastache foeniculum, a medicinal plant of the family Lamiaceae. Different explant types (cotyledons, hypocotyls, shoot tips, and nodal segments) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) in combination with 1 μM indole-3-acetic acid (IAA). Regenerated shoots were transferred to half- or full-strength MS medium with various growth regulators for root regeneration. Shoot regeneration frequency was affected by the explant type and BAP concentration. The highest shoot regeneration frequency was recorded for nodal explants followed by cotyledons, shoot tips, and hypocotyls. For nodal explants, the highest shoot regeneration frequencies (46.8–53.7) were recorded on media containing 4.4 μM BAP + 1 μM IAA or 8.8 μM BAP + 1 μM IAA. The lowest regeneration frequency (6.3), obtained on BAP-containing medium, was observed for hypocotyl explants cultured on medium containing 4.4 μM BAP + 1 μM IAA. Elongated shoots were rooted on all of the applied growth regulator treatments and media. The highest rooting rate (92%) was obtained on half-strength MS medium containing 1.1 μM IBA. The well-rooted plantlets were acclimatized successfully in a greenhouse and transferred to soil with 100% survival.

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Acknowledgments

We thank Dr. Abass Hassani of the Horticulture Department for providing the A. foeniculum seeds. We acknowledge the staff of the plant tissue culture laboratory of the Horticulture Department, especially Ms. Agaei, for their skillful technical assistance.

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Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, Urmia University, Nazloo Campus, P.O.Box 165, Urmia, Iran

    Leila Moharami & Bahman Hosseini

  2. Department of Medicinal and Industrial Plants, Institute of Biotechnology, Urmia University, Urmia, Iran

    Bahman Hosseini

  3. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, P.O. Box 76169133, Kerman, Iran

    Elnaz Ghotbi Ravandi

  4. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Nazloo Campus, P.O.Box 165, Urmia, Iran

    Morad Jafari

Authors
  1. Leila Moharami
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  2. Bahman Hosseini
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  3. Elnaz Ghotbi Ravandi
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  4. Morad Jafari
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Corresponding author

Correspondence to Bahman Hosseini.

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Editor: John Finer

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Moharami, L., Hosseini, B., Ghotbi Ravandi, E. et al. Effects of plant growth regulators and explant types on in vitro direct plant regeneration of Agastache foeniculum, an important medicinal plant. In Vitro Cell.Dev.Biol.-Plant 50, 707–711 (2014). https://doi.org/10.1007/s11627-014-9640-2

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  • DOI: https://doi.org/10.1007/s11627-014-9640-2

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